Laccase Production by Trameteshirsuta, Characterization, and Its Capability of Decoloring Chlorophyll

Liu, Jiayang; Liu, Wenhua; Cai, Yujie; Liao, Xiangru; Huang, Qingguo; Liang, Xiaohui

doi:10.33073/pjm-2014-043

Cited by 10 publications

(3 citation statements)

References 35 publications

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“…Previous investigations have shown that T. hirsuta produces very active laccase ( 45 , 46 ), which was also noted in this research. However, the F13 did not show LiP activity.…”

Section: Resultssupporting

confidence: 88%

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28

Jović

Buntić

Radovanović

et al. 2018

Food Technol. Biotechnol.

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SUMMARYThe aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T. hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84% with T. hirsuta F13 and 28.8% with S. gausapatum F28.

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“…Previous investigations have shown that T. hirsuta produces very active laccase ( 45 , 46 ), which was also noted in this research. However, the F13 did not show LiP activity.…”

Section: Resultssupporting

confidence: 88%

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28

Jović

Buntić

Radovanović

et al. 2018

Food Technol. Biotechnol.

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show abstract

“…The degradation efficiency, however, varies greatly depending on the single strains and species, as demonstrated in GC/MS tests. The causes may be different environmental growing conditions and substrate types, but especially adaptation to the presence of toxic agents such as pesticides, PAHs, and chlorinated agents [62][63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Trichoderma: Evaluation of Its Degrading Abilities for the Bioremediation of Hydrocarbon Complex Mixtures

et al. 2020

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Hydrocarbons can have very harmful effects on organisms and the environment, and conventional techniques for their removal are expensive and require the use of chemicals and long-term actions. Trichoderma is an ascomycete genus known to be active on different recalcitrant substrates, since it can produce a set of nonspecific extracellular enzymes generally involved in the degradation of lignin. However, the literature concerning the use of Trichoderma to degrade hydrocarbons is still limited. In this work we aimed to investigate the ability of Trichoderma to exploit used engine oil as its sole carbon source for prospective bioremediation of contaminated substrates. Four different strains belonging to Trichoderma asperellum and Trichoderma harzianum species were tested. The fungi were inoculated in direct contact with used engine oil, and after 45 days the samples were analyzed by gas chromatography/mass spectrometry (GC/MS). The results showed that all strains (except Trichoderma asperellum F1020) significantly changed the oil composition, decreasing the aromatic fraction in favor of the aliphatic one. T. harzianum F26, especially, showed a significant reduction of the BTEX (benzene, toluene, ethylbenzene, and the three xylene isomers) and alkylbenzenes fraction and an increase in short-chain aliphatics C1–C20. Enzymatic tests for laccase and peroxidase were also carried out, demonstrating that every strain seems to express a different mode of action.

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“…Laccases are known for their ability to function optimally under neutral to slightly acidic conditions [32]. The increased activity at 60°C might be attributed to the enzyme's ability to maintain its stability and catalytic e ciency at elevated temperatures, although further investigations into its thermal stability are warranted [33].…”

Section: Ph and Temperature Optimamentioning

confidence: 99%

A comprehensive Extraction-Precipitation Method and Characterization Study to Optimize Biogas Slurry Enzyme Recovery

Rana,

Naudiyal,

Bal

et al. 2024

Preprint

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Biogas slurry (BGS), or digestate, is a nutrient-rich byproduct of anaerobic digestion, containing essential macronutrients and micronutrients alongside organic matter. This slurry acts as a potent organic fertilizer, improving soil structure, water retention, and microbial health. Enzymes like amylase, alkaline protease, and laccase, along with lignocellulosic enzymes such as β-glucosidase, cellulase, and xylanase, play crucial roles in enhancing biogas production through the breakdown of complex organic compounds. The current study focuses on the isolation and precipitation of these enzymes present in biogas slurry and analyzes their activity and stability under different conditions. All enzymes were observed to exhibit higher activity in their intracellular forms compared to their extracellular counterparts with respect to enzymatic activity. Enzymatic activity was significantly increased, ranging from 4-fold to up to 14-fold, using ammonium sulphate precipitation. The optimal pH for amylase and laccase was found to be 6.0 and 7.0 respectively, while alkaline protease exhibited optimal activity at pH 8.0. All three enzymes displayed optimal activity at temperatures ranging between 40-60°C. These findings suggested that BGS is a potential source of these enzymes and that ammonium sulphate precipitation (0-80%) can be a successful method for concentrating these enzymes as well as understanding the optimal pH and temperature conditions for these enzymes is crucial for maximizing their effectiveness in various industrial applications.

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Laccase Production by Trameteshirsuta, Characterization, and Its Capability of Decoloring Chlorophyll

Cited by 10 publications

References 35 publications

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28

Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28

Trichoderma: Evaluation of Its Degrading Abilities for the Bioremediation of Hydrocarbon Complex Mixtures

A comprehensive Extraction-Precipitation Method and Characterization Study to Optimize Biogas Slurry Enzyme Recovery

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