Laccase–Prussian blue film–graphene doped carbon paste modified electrode for carbamate pesticides quantification

Oliveira, Thiago M.B.F.; Barroso, M. Fátima; Morais, Simone; Araújo, Mariana de; Freire, Cristina; Lima‐Neto, Pedro de; Correia, Adriana N.; Oliveira, M.B.P.P.; Delerue–Matos, Cristina

doi:10.1016/j.bios.2013.03.026

Cited by 56 publications

(22 citation statements)

References 28 publications

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“…The results achieved with the proposed biosensor are in agreement or compare favorably with those reported previously (scarce and mainly based on acetylcholinesterase) for CBR [54][55][56][57], PPX [56], FMT [10,13] and ZRM [13,58].…”

Section: Electroanalytical Characteristicssupporting

confidence: 79%

“…Subsequently, this paste was doped with 20% (w/w) of graphene. This proportion was selected based on prior studies developed by our team [13]. The resultant composite material showed excellent characteristics (high conductivity and electron transfer rate) as transducer.…”

Section: Bi-enzymatic Biosensor Constructionmentioning

confidence: 99%

“…In order to improve the current signal, the enrichment of CS matrix with metallic nanoparticles has shown interesting results [21,24,29,33]. Graphene shows great promise for the development of electrochemical biosensors due to its excellent mechanical flexibility, fast electron transfer, and good biocompatibility [13,[34][35][36]. In addition, its electrocatalytic action diminishes the overpotential associated to electroactive compounds, minimizing the interferences that occur in real samples [37].…”

Section: Introductionmentioning

confidence: 99%

“…As far as the authors know, there is no publication related to the application of bi-enzymatic biosensors for the quantification of pesticides in food commodities or in other real samples. Moreover, there is a general lack of validated biosensor-based procedures for analysis of food samples [1,12,13,26].…”

Section: Introductionmentioning

confidence: 99%

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Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Oliveira

Barroso

Araújo

et al. 2014

Bioelectrochemistry

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A bi-enzymatic biosensor (LACC-TYR-AuNPs-CS/GPE) for carbamates was prepared in a single step by electro-deposition of a hybrid film onto a graphene doped carbon paste electrode (GPE). Graphene and the gold nanopar-ticles (AuNPs) were morphologically characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, dynamic light scattering and laser Doppler velocimetry. The electrodeposited hybrid film was composed of laccase (LACC), tyrosinase (TYR) and AuNPs entrapped in a chitosan (CS) polymeric matrix. Exper-imental parameters, namely graphene redox state, AuNPs:CS ratio, enzymes concentration, pH and inhibition time were evaluated. LACC-TYR-AuNPs-CS/GPE exhibited an improved Michaelis-Menten kinetic constant (26.9 ± 0.5 M) when compared with LACC-AuNPs-CS/GPE (37.8 ± 0.2 M) and TYR-AuNPs-CS/GPE (52.3 ± 0.4 M). Using 4-aminophenol as substrate at pH 5.5, the device presented wide linear ranges, low detection limits (1.68 × 10 −9 ± 1.18× 10 −10 -2.15 × 10 −7 ± 3.41 × 10 −9 M), high accuracy, sensitivity (1.13 × 10 6 ± 8.11 × 10 4 -2.19 × 10 8 ± 2.51× 10 7 %inhibition M −1 ), repeatability (1.2-5.8% RSD), reproducibility (3.2-6.5% RSD) and stability (ca. twenty days) to determine carbaryl, formetanate hydrochloride, propoxur and ziram in citrus fruits based on their inhibitory capacity on the polyphenoloxidases activity. Recoveries at two fortified levels ranged from 93.8 ± 0.3% (lemon) to 97.8 ± 0.3% (orange). Glucose, citric acid and ascorbic acid do not interfere signifi-cantly in the electroanalysis. The proposed electroanalytical procedure can be a promising tool for food safety control.

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Section: Electroanalytical Characteristicssupporting

confidence: 79%

Section: Bi-enzymatic Biosensor Constructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Oliveira

Barroso

Araújo

et al. 2014

Bioelectrochemistry

Self Cite

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“…118 In spite of these remaining challenges, graphene has been used in many sensing tasks, including for detection of mercury ions, 119 specific genes, 120 and DNA, 121,122 in which the influence of graphene layers onto oxidation of DNA bases 123 has also been explored. Graphene has been found to affect the response of the oxidoreductase enzymes glucose oxidase (GOx), 124 horseradish peroxidase (HRP), 125 cytochrome c, 126 laccase, 127 and bilirubin oxidase. 128 It has also been reported as components in mimetic devices 129 and in FETs and bioFETs where changes in conductivity occur during detection.…”

Section: Graphenementioning

confidence: 99%

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Oliveira

Iost

Siqueira

et al. 2014

ACS Appl. Mater. Interfaces

156

137

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Clinical diagnosis has always been dependent on the efficient immobilization of biomolecules in solid matrices with preserved activity, but significant developments have taken place in recent years with the increasing control of molecular architecture in organized films. Of particular importance is the synergy achieved with distinct materials such as nanoparticles, antibodies, enzymes, and other nanostructures, forming structures organized on the nanoscale. In this review, emphasis will be placed on nanomaterials for biosensing based on molecular recognition, where the recognition element may be an enzyme, DNA, RNA, catalytic antibody, aptamer, and labeled biomolecule. All of these elements may be assembled in nanostructured films, whose layer-by-layer nature is essential for combining different properties in the same device. Sensing can be done with a number of optical, electrical, and electrochemical methods, which may also rely on nanostructures for enhanced performance, as is the case of reporting nanoparticles in bioelectronics devices. The successful design of such devices requires investigation of interface properties of functionalized surfaces, for which a variety of experimental and theoretical methods have been used. Because diagnosis involves the acquisition of large amounts of data, statistical and computational methods are now in widespread use, and one may envisage an integrated expert system where information from different sources may be mined to generate the diagnostics.

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Electrochemical Biosensors Based on Green Synthesized Graphene and Graphene Nanocomposites

Tabrizi

Marsal

2019

Handbook of Graphene

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Laccase–Prussian blue film–graphene doped carbon paste modified electrode for carbamate pesticides quantification

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Cited by 56 publications

References 28 publications

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

Nanomaterials for Diagnosis: Challenges and Applications in Smart Devices Based on Molecular Recognition

Electrochemical Biosensors Based on Green Synthesized Graphene and Graphene Nanocomposites

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