Lack of an association between a single nucleotide polymorphism in the bovine myogenic determination 1 (MyoD1) gene and meat quality traits in indigenous Chinese cattle breeds

Abstract

ABSTRACT. The myogenic determination 1 (MyoD1) gene is a member of the MyoD gene family. It encodes for skeletal musclespecific transcription factors containing highly conserved basic helixloop-helix regions that perform important roles in the initiation, maintenance, and regulation of phenotypic traits. We investigated a new single nucleotide polymorphism (SNP) in the MyoD1 gene to evaluate whether this polymorphism affects meat quality traits in five Chinese indigenous cattle breeds, namely Qinchuan (QC), Xia-Nan (XN), Nan-yang (NY), Luxi (LX), and Jia-xian red (JXR). A C→G transversion at position 624 was detected in exon 1 of the MyoD1 gene; it causes an amino acid substitution ( 624 serine/ 624 cysteine). Least squares analysis showed that this SNP is not significantly associated with back 2 test, the genotype distributions of four cattle breeds (LX, NY, QC, and XN) did not agree with Hardy-Weinberg equilibrium (P < 0.05); one breed (JXR) did not deviate significantly from Hardy-Weinberg equilibrium (P > 0.05). The genotypic frequencies among all five cattle breeds showed moderate diversity (0.25 < polymorphism information content < 0.5). We concluded that the C624G SNP of the MyoD1 gene does not influence meat quality traits in indigenous Chinese cattle breeds; however, this SNP could be included in breed composition and population admixture analyses due to the marked differences in allelic frequencies among these five breeds.

Polymorphism of MyoD1 and Myf6 genes and associations with carcass and meat quality traits in beef cattle

“…This result was similar to that of a previous study of association analysis of the TaqI-RFLP locus in intron 2; individuals with genotype AA had higher values of LW, CW, and LMA compared to individuals with the AB genotype (P < 0.05) (Tian et al, 2007). In contrast, Ujan et al (2011) found that the g.624C>G polymorphism locus in exon 1 had no influence on any meat quality traits in 5 indigenous Chinese cattle breeds. Several studies have demonstrated associations between MyoD1 and growth traits.…”

On-Farm and Processing Factors Affecting Rabbit Carcass and Meat Quality Attributes

“…In addition, the potential genetic improvement of rabbit meat characteristics is decided by the occurrence of specific genes in rabbits. Accordingly, Wang et al (2017) proposed myogenic factor 5 (Myf5) single nucleotide polymorphism (SNPs) could be considered as a potential genetic marker for good meat quality selection in breeding programmes of Ira and Tianfu Black breed since it plays significant roles in muscle fiber formation and transcription of muscle-specific genes (Ujan et al, 2011). Apart from Myf5, calpastatin and myopalladin (MYPN) could also be considered as candidate genes for the genetic improvement of rabbit meat traits (Li et al, 2018;Wang et al, 2017).…”

Population Structure and Selection Signal Analysis of Nanyang Cattle Based on Whole-Genome Sequencing Data

“…Recent investigations into economic traits of NY cattle have predominantly focused on the exploration of candidate genes. For instance, research has delved into the impact of genes like SIRT1 [ 6 ] and VEGF [ 7 ] on body weight gain, FBXO32 [ 8 ] and PAX3 [ 9 ] on body length, and MYOD1 [ 10 ] on meat quality. However, there is currently no identification of genes associated with adaptive traits in NY cattle on WGS data.…”