Lack of an HSP70 heat shock response in two Antarctic marine invertebrates

Clark, Melody S.; Fraser, Keiron P. P.

doi:10.1007/s00300-008-0447-7

Cited by 86 publications

(56 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar down-regulation of several genes (dnaja1, hsp60, hsp70_3 and gdh) at 10 compared to 5°C after LTS was detected in C. glacialis using qPCR. The lack of cellular stress response in Antarctic species is due to adaptation and specialization to stably cold Antarctic waters (Clark et al 2008, Bilyk & Cheng 2014. Although environmental conditions in the Arctic Ocean are more variable then in the Antarctic (Clarke & Peck 1991), and the lack of cellular stress response has not been reported in Arctic species, the lack of thermal stress response in C. glacialis may be explained by cold-water specialization.…”

Section: Lack Of Thermal Stress Response In C Glacialismentioning

confidence: 99%

See 1 more Smart Citation

Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Smolina¹,

Kollias²,

Møller³

et al. 2015

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Climate change has already led to the range expansion of warm-water plankton assemblages in the northeast Atlantic and the corresponding range contraction of colder-water species. The temperate copepod Calanus finmarchicus is predicted to shift farther northward into polar waters traditionally dominated by the arctic copepod C. glacialis. To identify temperaturemediated changes in gene expression that may be critical for the thermal acclimation and resilience of the 2 Calanus spp., we conducted a whole transcriptome profiling using RNA-seq on an Ion Torrent platform. Transcriptome responses of C. finmarchicus and C. glacialis from Disko Bay, west Greenland, were investigated under realistic thermal stresses (at + 5, +10 and +15°C) for 4 h and 6 d. C. finmarchicus showed a strong response to temperature and duration of stress, involving up-regulation of genes related to protein folding, transcription, translation and metabolism. In sharp contrast, C. glacialis displayed only low-magnitude changes in gene expression in response to temperature and duration of stress. Differences in the thermal responses of the 2 species, particularly the lack of thermal stress response in C. glacialis, are in line with laboratory and field observations and suggest a vulnerability of C. glacialis to climate change.

show abstract

Section: Lack Of Thermal Stress Response In C Glacialismentioning

confidence: 99%

“…Trematomus bernacchii) (Buckley & Somero 2009) to invertebrates (La Terza et al 2001, Clark et al 2008. In most cases only heat shock response, particularly expression of HSP70, was in - vestigated and absence of HSP up-regulation was detected.…”

Section: Lack Of Thermal Stress Response In C Glacialismentioning

confidence: 99%

Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Smolina¹,

Kollias²,

Møller³

et al. 2015

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…For example, the enhanced expression of GRP78 has been described in response to heat in diverse temperate species such as the Atlantic salmon Salmo salar (Lund et al 2002), the oyster Crassostrea gigas (Yokoyama et al 2006) and the crustacean Fenneropenaeus chinensis (Luan et al 2009). However, this mechanism is absent in mammalian and rainbow trout Oncorhynchus mykiss cell lines (Kozutsumi et al 1989;Ojima et al 2005) and the Antarctic invertebrates Odontaster validus and Paraceradochus gibber (Clark et al 2008d). There is now an increasing number of examples of both HSP70 and HSC70 being induced in response to heat in marine species (reviewed in Place et al 2008).…”

Section: Hsp70 Gene Family Expressionmentioning

confidence: 99%

Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)

Clark

Peck

2009

Cell Stress and Chaperones

Self Cite

View full text Add to dashboard Cite

The Antarctic limpet, Nacella concinna, exhibits the classical heat shock response, with up-regulation of duplicated forms of the inducible heat shock protein 70 (HSP70) gene in response to experimental manipulation of seawater temperatures. However, this response only occurs in the laboratory at temperatures well in excess of any experienced in the field. Subsequent environmental sampling of inter-tidal animals also showed up-regulation of these genes, but at temperature thresholds much lower than those required to elicit a response in the laboratory. It was hypothesised that this was a reflection of the complexity of the stresses encountered in the inter-tidal region. Here, we describe a further series of experiments comprising both laboratory manipulation and environmental sampling of N. concinna. We investigate the expression of HSP70 gene family members (HSP70A, HSP70B, GRP78 and HSC70) in response to a further suite of environmental stressors: seasonal and experimental cold, freshwater, desiccation, chronic heat and periodic emersion. Lowered temperatures (−1.9°C and −1.6°C), generally produced a downregulation of all HSP70 family members, with some upregulation of HSC70 when emerging from the winter period and increasing sea temperatures. There was no significant response to freshwater immersion. In response to acute and chronic heat treatments plus simulated tidal cycles, the data showed a clear pattern. HSP70A showed a strong but very short-term response to heat whilst the duplicated HSP70B also showed heat to be a trigger, but had a more sustained response to complex stresses. GRP78 expression indicates that it was acting as a generalised stress response under the experimental conditions described here. HSC70 was the major chaperone invoked in response to long-term stresses of varying types. These results provide intriguing clues not only to the complexity of HSP70 gene expression in response to environmental change but also insights into the stress response of a non-model species.

show abstract

“…Significant differences of Le-SOD expression levels between the groups of small individuals were identified using an unpaired t test with Welch correction for unequal variances or the nonparametric Mann-Whitney test pathways involving NFκB as found in the disk abalone Haliotis discus discus (De Zoysa et al 2009;Ju et al 2007). Whereas some Antarctic marine invertebrates such as the sea star Odontaster validus or the gammarid Paraceradocus gibber lack a classical heat shock response (Clark et al 2008b;Clark and Peck 2009), experimental work with L. elliptica indicated that the HSP70 genes are induced in response to heat stress (Clark et al 2008a;Park et al 2007), hypoxia (Clark and Peck 2009;Clark et al 2013), and also changes in pH (Cummings et al 2011). HSPs are often involved in stress defense and have been shown to be upregulated in hemocytes upon microbial challenge in bay scallops (Argopecten irradians) and zebra mussels (Dreissena polymorpha) (Xu and Faisal 2009;Song et al (2006).…”

mentioning

confidence: 99%

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Husmann

Abele

Rosenstiel

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

Increasing temperatures and glacier melting at the Western Antarctic Peninsula (WAP) are causing rapid changes in shallow coastal and shelf systems. Climate change-related rising water temperatures, enhanced ice scouring, as well as coastal sediment runoff, in combination with changing feeding conditions and microbial community composition, will affect all elements of the nearshore benthic ecosystem, a major component of which is the Antarctic soft-shelled clam Laternula elliptica. A 454-based RNA sequencing was carried out on tissues and hemocytes of L. elliptica, resulting in 42,525 contigs, of which 48 % was assigned putative functions. Changes in the expression of putative stress response genes were then investigated in hemocytes and siphon tissue of young and old animals subjected to starvation and injury experiments in order to investigate their response to sedimentation (food dilution and starvation) and iceberg scouring (injury). Analysis of antioxidant defense (Le-SOD and Lecatalase), wound repair (Le-TIMP and Le-chitinase), and stress and immune response (Le-HSP70, Le-actin, and Letheromacin) genes revealed that most transcripts were more clearly affected by injury rather than starvation. The upregulation of these genes was particularly high in the hemocytes of young, fed individuals after acute injury. Only minor changes in expression were detected in young animals under the selected starvation conditions and in older individuals. The stress response of L. elliptica thus depends on the nature of the environmental cue and on age. This has consequences for future population predictions as the environmental changes at the WAP will differentially impact L. elliptica age classes and is bound to alter population structure.

show abstract

Lack of an HSP70 heat shock response in two Antarctic marine invertebrates

Cited by 86 publications

References 41 publications

Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)

Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation

Contact Info

Product

Resources

About