Lack of Association between Hsa-Mir-499 rs3746444 Polymorphism and Cancer Risk: Meta-analysis Findings

Abstract

Epidemiologic findings concerning the association between the hsa-mir-499 rs3746444 A>G polymorphism and cancer risk have yielded mixed results. We aimed to investigate the association by performing a meta-analysis of all available studies. We searched PubMed and EMBASE for studies published up to November 2014, using odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of any association. The BenjaminiHochberg (BH) method was used to correct the p values for multiple comparisons. We included 39 studies, including 14,136 cases and 16,937 controls. The results of overall meta-analysis suggested a borderline association between hsa-mir-499 rs3746444 polymorphism and cancer susceptibility (AG+GG vs. AA: OR=1.15, 95% CI= 1.04-1.26, corrected p value=0.04). After removing studies not conforming to Hardy-Weinberg equilibrium (HWE), however, this association disappeared (AG+GG vs AA: OR=1.18, 95% CI=1.03-1.34, corrected p value=0.21). When stratified analysis by ethnicity, cancer type or HWE in controls, although some associations between hsa-mir-499 rs3746444 polymorphism and cancer susceptibility were detected, these associations no longer existed after adjustment using BH method. In conclusion, our meta-analysis suggests that hsa-mir-499 rs3746444 A>G polymorphism is not associated with risk of cancer based on current evidence. conclusive. Many meta-analyses have examined the association of the polymorphism with cancer risk and the lasted one suggested that hsa-mir-499 rs3746444 polymorphism was a risk factor for cancer development. Since the latest meta-analysis had been performed, nineteen case-control studies (Ling

The Associations of Single Nucleotide Polymorphisms in miR196a2, miR-499, and miR-608 With Breast Cancer Susceptibility

“…40 Although some meta-analytic results showed that the association was more notable in Asian populations than in Caucasian populations, 32,41 other meta-analyses found no significant association between rs3746444 and BC risk. 34,42 In our study, the results suggested that individuals who carried rs3746444 AG/GG genotypes had an increased BC risk. Moreover, the rs3746444 AG/GG genotypes were revealed to be associated with an increased risk of moderate-severe vascular invasion and positive HER-2 in BC.…”

A Meta-Analysis of miR-499 rs3746444 Polymorphism for Cancer Risk of Different Systems: Evidence From 65 Case-Control Studies

“…Several meta-analyses have explored the association between the miR-499 rs3746444 polymorphism and cancer risk and it is difficult to judge if the analysis without studies departing from HWE would be more valid or not. An overall meta-analysis by Jiang et al ( 2015 ) demonstrated a borderline association (TC+CC vs. TT: OR = 1.15, P = 0.04). However, the association disappeared (TC+CC vs. TT: OR = 1.18, P = 0.21) when studies departing from HWE were excluded.…”

The correlation ofmicroRNA-499rs3746444 T>C locus with the susceptibility of gastric cancer: from a case–control study to a meta-analysis

“…The relationship of mir-499 rs3746444 C-allele carriers with an increased susceptibility of cancer have been identified in some meta-analysis [8][9][10]12,13]. Of late, meta-analyses have tried to determine the correlation between this SNP and the risk of GC [8][9][10][11][12][13]32,33]. And all of them have obtained the null association, which maybe due to the limited literatures.…”

“…Rs3746444 T>C SNP is located in mir-499 and extensively studied the association of this SNP with cancer risk. Meta-analyses have established the correlation between this SNP and the risk of overall cancer [8][9][10][11][12][13]. Most of these findings suggested that rs3746444 C-allele carriers appeared to get an increased susceptibility of cancer [8][9][10]12,13].…”