Lack of Association between Polymorphisms of Hepatic Lipase with Lipid Profile in Young Jordanian Adults

Abstract

The human hepatic lipase (LIPC) gene encodes hepatic lipase, an enzyme involved in lipoprotein metabolism and regulation. Therefore, variants in LIPC gene may influence plasma lipoprotein levels. In this study, the association of LIPC C-514T and G-250A polymorphisms with plasma lipid profiles in 348 young Jordanians was investigated. Genotyping of C-514T and G-250A was performed by polymerase chain reaction and subsequent digestion with DraI and NiaIII restriction enzymes, respectively, while Roche analyzer was used to determine plasma total cholesterol, triglycerides, low-and high-density lipoprotein. The G-250 and C-514 alleles were most abundant in Jordanians with 79 and 80% frequencies, respectively. Additionally, no difference was found in the lipid–lipoprotein profile between the different genotype groups of C-514T or G-250A polymorphisms, even when males and females were examined separately (P > 0.05). In young Jordanian adults, the examined LIPC polymorphisms seem to play a limited role in determining the lipid profile.

Association of the ESR1 (rs9340799), OLR1 (rs3736234), LIPC (rs2070895), VDR (rs2228570), and CETP (rs708272) Polymorphisms With Risk of Coronary Artery Disease in Iranian Patients

“…The protein encoded by this gene is made up of 449 amino acids, and residue HL is responsible for controlling the metabolism of low‐density lipoprotein cholesterol (LDL‐c), intermediate‐density lipoprotein (IDL), and HDL‐c particles. Additionally, HL hydrolyzes TG and phospholipids [ 15 ]. A specific mutation, rs2070895 G‐250A in the intronic region of the LIPC gene, has been linked to changes in plasma lipid levels [ 16 ] and can account for about 20%–30% of the individual variance in HL activity [ 17 , 18 ].…”

Repercussões do polimorfismo -250G/A da lipase hepática sobre o metabolismo das lipoproteínas plasmáticas e a aterosclerose carotídea em amostra populacional brasileira normolipidêmica e assintomática

“…como a jordaniana (G = 0.79; A = 0.21)(198), americana (G = 0.53-0.79; A = 0.21-0.47)(199)(200), chinesa (G = 0.73; A = 0.27)(164), japonesa (G = 0.53; A = 0.47)(201), espanhola (G = 0.79; A = 0.21)(202), finlandesa (G = 0.76; A = 0.24)(179,197), austríaca (G = 0.86-0.79; A = 0.14-0.21) (130) e coreana (G = 0.63; A = 0.37)(203). Na população Brasileira, uma das mais heterogêneas no mundo, de Andrade e colaboradores(204) observaram, em um subgrupo de indivíduos do sul do país, que a frequência do alelo A variou entre 0.25 e 0.30.…”