Lack of association of brucellosis resistance with (GT)13 microsatellite allele at 3′UTR of NRAMP1 gene in Indian zebu (Bos indicus) and crossbred (Bos indicus×Bos taurus) cattle

Kumar, Nishant; Mitra, Abhijit; Ganguly, Indrajit; Singh, Rajendra; Deb, S. M.; Srivastava, S. K.; Sharma, Arjava

doi:10.1016/j.vetmic.2005.09.012

Cited by 36 publications

(28 citation statements)

References 19 publications

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“…Paixao et al (32) found that Holstein (Bos taurus taurus) and Indian zebu (Bos taurus indicus) subjects resistant to brucellosis display the 3Ј UTR Nramp1 genotype associated with resistance to the disease. On the contrary, Kumar et al (26) found that, in the Indian zebu and crossbred (Bos taurus indicus ϫ Bos taurus taurus) cattle, the same genotype is not associated with resistance to brucellosis. The Holstein animals screened by Paixao et al (32) (81 animals) and the zebu or crossbred animals tested by Kumar et al (26) (100 animals) were all homozygous for the allele conferring resistance.…”

Section: Discussionmentioning

confidence: 96%

“…On the contrary, Kumar et al (26) found that, in the Indian zebu and crossbred (Bos taurus indicus ϫ Bos taurus taurus) cattle, the same genotype is not associated with resistance to brucellosis. The Holstein animals screened by Paixao et al (32) (81 animals) and the zebu or crossbred animals tested by Kumar et al (26) (100 animals) were all homozygous for the allele conferring resistance. In the absence of data showing that alleles at unrelated loci are in Hardy-Weinberg equilibrium in cases and controls, the excess of homozygosity at the Nramp1 locus points to either mistyping of genotypes or population stratification (33,37).…”

Section: Discussionmentioning

confidence: 96%

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Protective Effect of the Nramp1 BB Genotype againstBrucella abortusin the Water Buffalo (Bubalus bubalis)

et al. 2007

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We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3 untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Protective Effect of the Nramp1 BB Genotype againstBrucella abortusin the Water Buffalo (Bubalus bubalis)

et al. 2007

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“…This is a highly conserved gene across mammals and is associated with resistance and susceptibility to various intracellular pathogens in humans as well as in livestock species (Blackwell et al 2001;Thomas and Joseph 2012). Previous studies have shown that microsatellite alleles localized in the 3 0 UTR of the SLC11A1 are involved in macrophage function and resistance to Brucella abortus infections in both cattle and buffalo (Kumar et al 2005;Borriello et al 2006;Capparelli et al 2007;Ganguly et al 2008;Martinez et al 2008;Kumar et al 2011). Another gene ATP8B2 contains STR in its exon.…”

Section: Strs Overlap With Genesmentioning

confidence: 99%

0309 Systematic profiling of short tandem repeats in the cattle genome

Liu

Haasl

et al. 2016

Journal of Animal Science

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Short tandem repeats (STRs), or microsatellites, are genetic variants with repetitive 2-6 base pair motifs in many mammalian genomes. Using high-throughput sequencing and experimental validations, we systematically profiled STRs in five Holsteins. We identified a total of 60,106 microsatellites and generated the first high-resolution STR map, representing a substantial pool of polymorphism in dairy cattle. We observed significant STRs overlap with functional genes and quantitative trait loci (QTL). We performed evolutionary and population genetic analyses using over 20,000 common dinucleotide STRs. Besides corroborating the well-established positive correlation between allele size and variance in allele size, these analyses also identified dozens of outlier STRs based on two anomalous relationships that counter expected characteristics of neutral evolution. And one STR locus overlaps with a significant region of a summary statistic designed to detect STR-related selection. Additionally, our results showed that only 57.1% of STRs located within SNP-based linkage disequilibrium (LD) blocks whereas the other 42.9% were out of blocks. Therefore, a substantial number of STRs are not tagged by SNPs in the cattle genome, likely due to STR's distinct mutation mechanism and elevated polymorphism. This study provides the foundation for future STR-based studies of cattle genome evolution and selection.

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“…Genes play one of the major roles in disease resistance in bovine species [3][4][5][6][7][8][9][10]. Candidate genes such as BoLA-DRB3 [11,12], FEZ [13], TLR4 [14,15], CARD 15 [16,17], cluster of differentiation 14 (CD14) [18][19][20] and many more genes are associated with mastitis resistance/susceptibility in different cattle breeds.…”

Section: Introductionmentioning

confidence: 99%

Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan fries cattle

Selvan¹,

Gupta²,

Chaudhari³

et al. 2014

Vet World

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Aim: The present study was undertaken with the objectives to characterize, identify DNA polymorphism in cluster of differentiation 14 (CD14) gene in Karan Fries (KF) cattle and to analyze association between genetic variants with incidence of clinical mastitis in National Dairy Research Institute (NDRI) herd, Karnal. Materials and Methods:Genomic DNA was extracted using blood of randomly selected hundred KF lactating cattle by phenol-chloroform method. After checking its quality and quantity, polymerase chain reaction (PCR) was carried out using reported primers to amplify 832 base pair region covering nucleotide base position number 1012 to 1843 (part of promoter, 5'UTR, exon 1, intron 1 and part of exon 2) of bovine CD14 gene. The PCR amplified target product was purified, sequenced and further ClustalW analysis was done to align edited sequence with reported Bos taurus sequence (EU148610.1). The restriction fragment length polymorphism (RFLP) analysis was performed for each KF cow using HinfI restriction enzyme (RE). Cows were assigned genotypes obtained by PCR-RFLP analysis and association study was done using Chi-square (χ 2 ) test.Results: After PCR amplification, DNA sequencing of amplicon confirmed the 832 bases covering 1012 to 1843 nucleotide base position of bovine CD14 gene. ClustalW multiple sequence alignment program for DNA revealed six nucleotide changes in KF cows at positions T1117D, T1239G, T1291C, G1359C, G1361A, and G1811A. Cows were also screened using PCR-RFLP with HinfI RE, which revealed three genotypes CC, CD and DD that differed significantly regarding mastitis incidence. Within CC genotype, 72.73% of cows were in a mastitis non-affected group whereas, those in CD and DD genotypes 69.44% and 60.38% respectively were mastitis affected. Conclusion:KF cows with allele C of CD14 gene were less susceptibility to mastitis compared with D allele.

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Lack of association of brucellosis resistance with (GT)13 microsatellite allele at 3′UTR of NRAMP1 gene in Indian zebu (Bos indicus) and crossbred (Bos indicus×Bos taurus) cattle

Cited by 36 publications

References 19 publications

Protective Effect of the Nramp1 BB Genotype againstBrucella abortusin the Water Buffalo (Bubalus bubalis)

Protective Effect of the Nramp1 BB Genotype againstBrucella abortusin the Water Buffalo (Bubalus bubalis)

0309 Systematic profiling of short tandem repeats in the cattle genome

Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan fries cattle

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