Lack of association of CPT1A polymorphisms or haplotypes on hepatic lipid content or insulin resistance in Japanese individuals with type 2 diabetes mellitus

Hirota, Yushi; Ohara, Takeshi; Zenibayashi, Masako; Kuno, Shin-ichi; Fukuyama, Keiko; Teranishi, Tetsuya; Kouyama, Kunichi; Miyake, Kazuaki; Maeda, Eiichi; Kasuga, Masato

doi:10.1016/j.metabol.2006.12.014

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Genomic DNA was extracted from peripheral blood lymphocytes using standard procedures, and polymerase chain reaction (PCR) amplifi cation was performed. Genotyping was carried out using allele-specifi c primer and intercalatormediated fl uorescence resonance energy transfer probe methods as described previously (Yamada et al, 2003, Hirota et al, 2007. Details of primer sequences and PCR conditions are listed in Table 1.…”

Section: Genotype Analysismentioning

confidence: 99%

Polymorphisms in Genes Involved in Inflammatory Pathways in Patients with Sudden Sensorineural Hearing Loss

Hiramatsu

Teranishi

Uchida

et al. 2012

Journal of Neurogenetics

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Although the etiology of idiopathic sudden sensorineural hearing loss (SSNHL) remains unclear, the pathologically increased permeability of blood vessels, elucidated by gadolinium-enhanced magnetic resonance imaging (MRI), suggests the involvement of inflammation. Because SSNHL is considered a multifactorial disease, possibly caused by interactions between genetic factors and environmental factors, the authors investigated the associations of polymorphisms of inflammatory mediator genes with susceptibility to SSNHL. The authors compared 72 patients affected by SSNHL and 2010 adults (1010 men and 1000 women; mean age 59.2 years; range 40-79) who participated in the National Institute for Longevity Sciences Longitudinal Study of Aging. Multiple logistic regression was used to obtain odds ratios (ORs) for SSNHL in subjects with polymorphisms in the genes IL-6 C - 572G, IL-4R G1902A, IL-10 A - 592C, TNFα C - 863A, TNFRSF1B G593A, VEGF C936T, VEGF C - 2578A, and VEGF G - 1154A, with adjustment for age, gender, and any history of hypertension, diabetes, or dyslipidemia. The per-allele OR for the risk of SSNHL in subjects bearing IL-6 C - 572G was 1.480 (95% confidence interval [CI], 1.037-2.111) in model 1 (no adjustment), 1.463 (CI, 1.022-2.094) in model 2 (adjusted for age and gender), and 1.460 (CI, 1.016-2.097) in model 3 (adjusted for age, gender, and a history of hypertension, diabetes, or dyslipidemia). Under the dominant model of inheritance, the ORs were 1.734 (CI, 1.080-2.783) in model 1, 1.690 (CI, 1.050-2.721) in model 2, and 1.669 (CI, 1.035-2.692) in model 3. The remaining seven polymorphisms failed to show any associations with the risk of SSNHL. These data need to be confirmed on larger series of patients. In conclusion, the IL-6 C - 572G polymorphism is associated with a risk of SSNHL. Because permeability of blood vessels in the inner ear is frequently increased in patients with SSNHL, inflammation of the inner ear might be involved.

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Section: Genotype Analysismentioning

confidence: 99%

Polymorphisms in Genes Involved in Inflammatory Pathways in Patients with Sudden Sensorineural Hearing Loss

Hiramatsu

Teranishi

Uchida

et al. 2012

Journal of Neurogenetics

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“…Genomic DNA was extracted from peripheral blood lymphocytes using standard procedures, and polymerase chain reaction (PCR) amplification was performed. Genotyping was carried out using an allele-specific primer method and an intercalator-mediated fluorescence resonance energy transfer probe method, as described previously (Yamada et al, 2003;Hirota et al, 2007). Details of primer sequences and PCR conditions are listed in Table 1.…”

Section: Genotype Analysismentioning

confidence: 99%

Polymorphisms in Genes Involved in Oxidative Stress Response in Patients with Sudden Sensorineural Hearing Loss and Ménière's Disease in a Japanese Population

Teranishi

Uchida²,

Nishio³

et al. 2012

DNA and Cell Biology

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“…Hirota and colleagues ( 54 ) found no association between CPT1A polymorphisms and obesity or fasting lipid phenotypes in Japanese individuals with T2D. In a cross-sectional cohort of French-Canadians, Robitaille et al reported an association between the nonsynonymous A275T (rs17610395) SNP with BMI ( P = 0.05) and waist circumference ( P = 0.008) only after accounting for dietary fat intake ( 53 ). Finally, in Greenland Inuit, Rajakumar et al showed the L479 allele in the nonsynonymous P479L variant was associated with elevated fasting HDL-cholesterol and ApoA1 levels ( 35 ).…”

mentioning

confidence: 98%

“…Three studies have previously investigated the infl uence of CPT1A polymorphisms on obesity and lipid phenotypes in humans ( 35,53,54 ). Hirota and colleagues ( 54 ) found no association between CPT1A polymorphisms and obesity or fasting lipid phenotypes in Japanese individuals with T2D.…”

mentioning

confidence: 99%

Genetic polymorphisms in carnitine palmitoyltransferase 1A gene are associated with variation in body composition and fasting lipid traits in Yup'ik Eskimos

Lemas

Wiener

O’Brien

et al. 2012

Journal of Lipid Research

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Obesity is associated with a series of metabolic conditions clinically referred to as metabolic syndrome, which includes hypertension, dyslipidemia, hyperglycemia, and the development of type 2 diabetes (T2D). Approximately sixty percent of obese individuals have metabolic complications ( 1 ); however, "healthy obese" individuals have been identifi ed with excessive accumulation of body fat that does not translate to dyslipidemia and insulin resistance ( 2, 3 ). For example, some Eskimo/Inuit people indigenous to Alaska are obese, but they have historically demonstrated low prevalence of insulin resistance, metabolic syndrome, and T2D ( 4-7 ). Specifi cally, Yup'ik Eskimo peoples living in Southwest Alaska have obesity prevalence comparable to the general US population, yet the prevalence of metabolic syndrome ( 8 ) and T2D ( 9 ) is signifi cantly less than that observed in the general US population ( 10, 11 ). Although the mechanisms that allow Abstract Variants of carnitine palmitoyltransferase 1A ( CPT1A ), a key hepatic lipid oxidation enzyme, may infl uence how fatty acid oxidation contributes to obesity and metabolic outcomes. CPT1A is regulated by diet, suggesting interactions between gene variants and diet may infl uence outcomes. The objective of this study was to test the association of CPT1A variants with body composition and lipids, mediated by consumption of polyunsaturated fatty acids (PUFA). Obesity phenotypes and fasting lipids were measured in a cross-sectional sample of Yup'ik Eskimo individuals (n = 1141) from the Center of Alaska Native Health Research (CANHR) study. Twenty-eight tagging CPT1A SNPs were evaluated with outcomes of interest in regression models accounting for family structure. Several CPT1A polymorphisms were associated with HDL-cholesterol and obesity phenotypes. The P479L (rs80356779) variant was associated with all obesity-related traits and fasting HDLcholesterol. Interestingly, the association of P479L with HDL-cholesterol was still signifi cant after correcting for body mass index (BMI), percentage body fat (PBF), or waist circumference (WC). Our fi ndings are consistent with the hypothesis that the L479 allele of the CPT1A P479L variant confers a selective advantage that is both cardioprotective (through increased HDL-cholesterol) and associated with reduced adiposity. -Lemas, D.

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Lack of association of CPT1A polymorphisms or haplotypes on hepatic lipid content or insulin resistance in Japanese individuals with type 2 diabetes mellitus

Cited by 18 publications

References 32 publications

Polymorphisms in Genes Involved in Inflammatory Pathways in Patients with Sudden Sensorineural Hearing Loss

Polymorphisms in Genes Involved in Inflammatory Pathways in Patients with Sudden Sensorineural Hearing Loss

Polymorphisms in Genes Involved in Oxidative Stress Response in Patients with Sudden Sensorineural Hearing Loss and Ménière's Disease in a Japanese Population

Genetic polymorphisms in carnitine palmitoyltransferase 1A gene are associated with variation in body composition and fasting lipid traits in Yup'ik Eskimos

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