Lack of Conserved miRNA Deregulation in HPV-Induced Squamous Cell Carcinomas

Nunvář, Jaroslav; Pagáčová, Lucie; Vojtechova, Zuzana; Azevedo, Nayara; Smahelova, Jana; Saláková, Martina; Tachezy, Ruth

doi:10.3390/biom11050764

Cited by 6 publications

(5 citation statements)

References 119 publications

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“…To date, only a few studies have analyzed miRNA expression in HPV-related and HPV-unrelated oropharyngeal cancers [15,33,34], where no commonly shared miRNA dysregulations have been identified across studies [15,33,34]. While previous investigations have examined the presence of TERTp mutations in various subsites of oral and oropharyngeal cancers [35,36], specific miRNAs linked to TERTp mutations in tonsil cancers and their correlation with HPV have been sparingly explored.…”

Section: Discussionmentioning

confidence: 99%

Dysregulated microRNA Expression Relevant to TERT Promoter Mutations in Tonsil Cancer—A Pilot Study

Kwon,

Park,

Lee

et al. 2023

Life

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Tonsillar squamous cell carcinomas (TSCCs) exhibit high rates of human papillomavirus (HPV) positivity. The expression profiles of microRNA (miRNA), which are small RNA molecules that play pivotal roles in biological processes, in TSCC in relation to the HPV status and cancer-related genetic mutations are not well investigated. Herein, we expanded our previous research, which was focused on established clinicopathological and genetic mutational data, to profile miRNA expression in TSCC, aiming to identify clinically relevant targets for early diagnosis and therapeutic intervention. The miRNA profiles were analyzed using the nCounter Nanostring miRNA Expression assay in 22 surgically resected TSCC tissues and their contralateral normal tonsil tissues. The TERT promoter (TERTp) gene was the only relevant candidate gene associated with differentially expressed miRNAs in TSCC. Hierarchical clustering analysis revealed high expression levels of hsa-miR-1285-5p, hsa-miR-1203, hsa-miR-663a, hsa-miR-1303, hsa-miR-33a-5p, and hsa-miR-3615 coupled with low expression levels of hsa-miR-3182, hsa-miR-219a-2-3p, and hsa-miR-767-3p, which were associated with HPV-positive TSCC (p = 0.009). Functional enrichment analysis revealed that these dysregulated miRNAs tended to be involved in protein binding (molecular function) and cellular components (biological processes). Therefore, hsa-miR-1285-5p and hsa-miR-663a may be associated with HPV-positive TERTp-mutated tumors and may serve as potential treatment targets and biomarkers for early detection.

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Section: Discussionmentioning

confidence: 99%

Dysregulated microRNA Expression Relevant to TERT Promoter Mutations in Tonsil Cancer—A Pilot Study

Kwon,

Park,

Lee

et al. 2023

Life

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“…However, the highest number of miRNAs differentially expressed was detected in cervical HPV + tumors compared to the other SCCs. They also found more downregulated than upregulated miRNAs in HPV + cervical tumors (Nunvar et al, 2021).…”

Section: Hpv and Mirnasmentioning

confidence: 92%

Cervical Cancer, Papillomavirus, and miRNA Dysfunction

Bañuelos-Villegas¹,

Perez-Y-Perez²,

Álvarez-Salas³

2021

Front. Mol. Biosci.

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Cervical cancer is the leading cause of death by cancer in women from developing countries. Persistent infection with high-risk human papillomavirus (HPV) types 16 and 18 is a major risk factor for cervical carcinogenesis. Nevertheless, only a few women with morphologic expression of HPV infection progress into invasive disease suggesting the involvement of other factors in cervical carcinogenesis. MicroRNAs (miRNAs) are conserved small non-coding RNAs that negatively regulate gene expression including genes involved in fundamental biological processes and human cancer. Dysregulation of miRNAs has been widely reported in cervical cancer. This work focuses on reviewing the miRNAs affected during the HPV infection process, as well relevant miRNAs that contribute to the development and maintenance of malignant cervical tumor cells. Finally, we recapitulate on miRNAs that may be used to distinguish between healthy individuals from patients with precancerous lesions or cervical tumors.

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“…MiRNA profiles obtained from TCGA data through NGS could screen differentially expressed miRNAs in HPV-positive and HPV-negative HNSCC tissues. Nunvar et al (35) have reported that 70 and 116 specific miRNAs were differently expressed in HPV-positive and HPV-negative HNSCC, as identified by NGS.…”

Section: Expression and Significance Of Mirnas In Hpv-positive Hnsccmentioning

confidence: 99%

“…Specifically, the expression level of certain miRNAs has been associated with HPV infection. As HPV integrates into the host genome, the viral oncoproteins can modulate the expression of host genes and may also perturb the level of miRNAs ( 23 , 35 ). Some miRNAs can induce abnormal cell cycle, affect cell apoptosis, or even alter genomic stability, thereby affecting the radiotherapy sensitivity ( 36 ).…”

Section: Micrornamentioning

confidence: 99%

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Expression and molecular regulation of non-coding RNAs in HPV-positive head and neck squamous cell carcinoma

Guo

Yang

et al. 2023

Front. Oncol.

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Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent malignancy worldwide. Accumulating evidence suggests that persistent HPV infection is closely related to a subset of HNSCC types, and the incidence of human papillomavirus (HPV)-positive HNSCC has been annually increasing in recent decades. Although the carcinogenesis of HPV-positive HNSCC has not been completely elucidated, it has been well confirmed that E6 and E7, the main viral oncoproteins are responsible for the maintenance of malignant transformation, promotion of cell proliferation, and increase in tumor invasion. Moreover, compared with HPV-negative HNSCC, HPV-positive HNSCC shows some special clinical-pathological features, which are possibly related to HPV infection and their specific regulatory mechanisms. Non-coding RNA (ncRNA) is a class of RNA lacking the protein-coding function and playing a critical regulatory role via multiple complex molecular mechanisms. NcRNA is an important regulatory pattern of epigenetic modification, which can exert significant effects on HPV-induced tumorigenesis and progression by deregulating downstream genes. However, the knowledge of ncRNAs is still limited, hence, a better understanding of ncRNAs could provide some insights for exploring the carcinogenesis mechanism and identifying valuable biomarkers in HPV-positive HNSCC. Therefore, in this review, we mainly focused on the expression profile of ncRNAs (including lncRNA, miRNA, and circRNA) and explored their regulatory role in HPV-positive HNSCC, aiming to clarify the regulatory mechanism of ncRNAs and identify valuable biomarkers for HPV-positive HNSCC.

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Lack of Conserved miRNA Deregulation in HPV-Induced Squamous Cell Carcinomas

Cited by 6 publications

References 119 publications

Dysregulated microRNA Expression Relevant to TERT Promoter Mutations in Tonsil Cancer—A Pilot Study

Dysregulated microRNA Expression Relevant to TERT Promoter Mutations in Tonsil Cancer—A Pilot Study

Cervical Cancer, Papillomavirus, and miRNA Dysfunction

Expression and molecular regulation of non-coding RNAs in HPV-positive head and neck squamous cell carcinoma

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