Lack of correlation between lymphocyte activating determinants and HLA-DR on acute leukaemias

Taylor, Geoffrey; Ridway, J C; Fergusson, William; Harris, Rodney

doi:10.1038/bjc.1984.76

Cited by 6 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lymphoid cell lines were prepared by immortalising lymphocytes using the B95-8 strain of Epstein-Barr virus.4 Mixed lymphocyte cultures were carried out as previously described. 5 HLA class II alleles were typed by restriction fragment length polymorphism analysis. 6 Typing of HLA-DPB1 and DRB1 alleles was carried out by the reverse polymerase chain reactionsequence specific oligonucleotide technique.7 Analysis of engraftment after transplant was carried out by the single strand conformation polymorphism (SSCP) method of Orita et al8…”

Section: Methodsmentioning

confidence: 99%

Infantile osteopetrosis; bone marrow transplantation from a cousin donor.

Taylor

Dearden

Will

et al. 1995

Archives of Disease in Childhood

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Infantile osteopetrosis; bone marrow transplantation from a cousin donor.

Taylor

Dearden

Will

et al. 1995

Archives of Disease in Childhood

Self Cite

View full text Add to dashboard Cite

show abstract

DR antigens expressed on tumor cells do not contribute to the blastogenetic response of autologous T cells

Vánky

Klein

Willems

1985

Cancer Immunol Immunother

View full text Add to dashboard Cite

Tumor cell suspensions prepared from surgical specimens were characterized for cellular composition and reactivity with monoclonal antibodies detecting T lymphocytes, monocytes, and the monomorphic determinants of DR molecules (antigens encoded by the D region of the major histocompatibility complex in man). About half the adenocarcinoma preparations contained tumor cells which expressed DR antigens. Lymphocytes of certain patients were stimulated in vitro by the autologous tumor cells, and this was independent of the expression of DR antigens on the tumor cells. In addition, pretreatment of the stimulator tumor cells with anti-DR Mab (monoclonal antibody) had only marginal effect on their stimulatory potential. In contrast, when the same tumor cells were used as stimulators of allogeneic lymphocytes, proliferation was more often seen with DR-positive tumors and the reaction was often inhibited by the anti-DR Mab treatment. There were exceptions, however, which suggest that other DR antigens not detected by the reagents used may have been expressed on these cells. The allostimulatory capacity of the tumor cells was usually weak and did not occur with all responder lymphocytes. It is important to note that stimulation of autologous lymphocytes could occur with tumor preparations that did not elicit allogeneic response. Thus, the in vitro stimulation of autologous blood-derived T cells by suspensions of unpropagated cells separated from solid tumors reflects the sensitization state of the patients against their tumor cells.

show abstract

Importance of MHC antigen expression on solid tumors in the in vitro interaction with autologous blood lymphocytes

Vánky

Stuber

Willems

et al. 1988

Cancer Immunol Immunother

View full text Add to dashboard Cite

In 54 patients with lung and 14 with ovarian carcinomas the quantitative variations of the major histocompatibility complex (MHC)-determined class I and class II antigens of the tumor cells were related to their in vitro interaction with blood lymphocytes, to the lymphoid infiltration of the tumors, and to the metastatic state of the disease. The tumor cell-lymphocyte interaction was measured by the proliferative response in mixed lymphocyte tumor cell culture and by the cytotoxic activity of the lymphocytes. The results showed that (1) none of the 23 tumors from patients with disseminated disease were lysed; (2) class I-negative tumors were not damaged; (3) lymphoid infiltration was present in a higher proportion of class II-positive tumors; and (4) both MHC-positive and -negative tumors were found among the disseminated tumors. The requirement of class I expression in the lytic interaction substantiates our earlier conclusion concerning the cytotoxic T lymphocyte nature of lymphocyte-mediated auto-tumor lysis. The lack of auto-tumor lysis in patients with metastases suggests impairment of lymphocyte function in advanced stages of the disease.

show abstract

Lack of correlation between lymphocyte activating determinants and HLA-DR on acute leukaemias

Cited by 6 publications

References 21 publications

Infantile osteopetrosis; bone marrow transplantation from a cousin donor.

Infantile osteopetrosis; bone marrow transplantation from a cousin donor.

DR antigens expressed on tumor cells do not contribute to the blastogenetic response of autologous T cells

Importance of MHC antigen expression on solid tumors in the in vitro interaction with autologous blood lymphocytes

Contact Info

Product

Resources

About