Lack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models

Boyle, M.A.; O’Donnell, Mary; Russell, R.J.; Coleman, David C.

doi:10.1016/j.jdent.2010.08.005

Cited by 18 publications

(25 citation statements)

References 60 publications

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“…(Boyle et al 2010;Eirheim et al 2004) but not to investigate the genotoxic effects of chemicals. The cells have, in nonconfluent cultures, an oval shape and possess large nuclei, their morphology resembling that of differentiated epithelial cells from the buccal cavity.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells

et al. 2012

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Glyphosate (G) is the largest selling herbicide worldwide; the most common formulations (Roundup, R) contain polyoxyethyleneamine as main surfactant. Recent findings indicate that G exposure may cause DNA damage and cancer in humans. Aim of this investigation was to study the cytotoxic and genotoxic properties of G and R (UltraMax) in a buccal epithelial cell line (TR146), as workers are exposed via inhalation to the herbicide. R induced acute cytotoxic effects at concentrations > 40 mg/l after 20 min, which were due to membrane damage and impairment of mitochondrial functions. With G, increased release of extracellular lactate dehydrogenase indicative for membrane damage was observed at doses > 80 mg/l. Both G and R induced DNA migration in single-cell gel electrophoresis assays at doses > 20 mg/l. Furthermore, an increase of nuclear aberrations that reflect DNA damage was observed. The frequencies of micronuclei and nuclear buds were elevated after 20-min exposure to 10-20 mg/l, while nucleoplasmatic bridges were only enhanced by R at the highest dose (20 mg/l). R was under all conditions more active than its active principle (G). Comparisons with results of earlier studies with lymphocytes and cells from internal organs indicate that epithelial cells are more susceptible to the cytotoxic and DNA-damaging properties of the herbicide and its formulation. Since we found genotoxic effects after short exposure to concentrations that correspond to a 450-fold dilution of spraying used in agriculture, our findings indicate that inhalation may cause DNA damage in exposed individuals.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells

et al. 2012

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“…However, a considerable number of these studies were carried out using model systems with DUWL tubing in the laboratory, few investigated the effectiveness of DUWL treatment agents in actual dental units and even fewer in dental units used for daily dental treatment (Tuttlebee et al ., 2002 ;Walker et al ., 2003 ;McDowell et al ., 2004 ;Spratt et al ., 2004 ;O'Donnell et al ., 2006O'Donnell et al ., , 2007O'Donnell et al ., , 2009O'Donnell et al ., , 2011Schel et al ., 2006 ;Coleman et al ., 2009 ). A minority of studies examined the continuous effectiveness of DUWL treatment agents in working dental units in the long-term (O'Donnell et al ., 2006Boyle et al ., 2010 ). Table 8.2 lists the range of chemical DUWL treatment agents that have been tested in actual dental units.…”

Section: Chemical and Disinfectant Duwl Treatmentsmentioning

confidence: 99%

“…pH range of products 2.0-7.4. pH neutral products are best as they do not show adverse effects on dental unit components (Kohno et al ., 2004 ;Martin and Gallagher, 2005 ;Zhang et al ., 2007 ;O'Donnell et al ., 2009 ;Boyle et al ., 2010 ). The pH neutral electrochemically activated solution Ecasol shown to lack cytotoxicity for human keratinocytes and reconstituted human oral epithelium .…”

Section: Electrochemically Activated Solutions (R)mentioning

confidence: 99%

“…biofi lm and maintaining good quality output water when used as residual treatments (Kohno et al ., 2004 ;Martin and Gallagher, 2005 ;Zhang et al ., 2007 ;O'Donnell et al ., 2009 ;Boyle et al ., 2010 ). Studies have shown that some of the most effective DUWL treatment agents contain hydrogen peroxide, hydrogen peroxide and silver or pH neutral electrochemically activated hypochlorous acid Tuttlebee et al ., 2002 ;Coleman et al ., 2009 ;O'Donnell et al ., 2009 ;Boyle et al ., 2010 ).…”

Section: Sodium Fl Uoride (P)mentioning

confidence: 99%

“…Studies have shown that some of the most effective DUWL treatment agents contain hydrogen peroxide, hydrogen peroxide and silver or pH neutral electrochemically activated hypochlorous acid Tuttlebee et al ., 2002 ;Coleman et al ., 2009 ;O'Donnell et al ., 2009 ;Boyle et al ., 2010 ). In general, residual DUWL treatment agents are more effective than agents used intermittently at eradicating DUWL biofi lm and maintaining good quality output water probably because they prevent the build-up of biofi lm within DUWLs and destroy planktonic organisms on an ongoing basis.…”

Section: Sodium Fl Uoride (P)mentioning

confidence: 99%

See 2 more Smart Citations

Minimising microbial contamination in dental unit water systems and microbial control in dental hospitals

Coleman

O’Donnell

Miller

et al. 2014

Decontamination in Hospitals and Healthcare

Self Cite

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Antimicrobial action, cytotoxicity and erosive potential of hypochlorous acid obtained from an electrolytic device compared with sodium hypochlorite

Souza,

Steier,

Vanin

et al. 2024

Clin Oral Invest

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Objectives: This study aimed to compare the antimicrobial action, cytotoxicity, cleaning ability, and erosion of dentine of hypochlorous acid (HClO) obtained from an electrolytic device at two different concentrations (Dentaqua) and three concentrations of sodium hypochlorite (NaOCl).Methods: Microbiological test -The root canals of sixty single-rooted extracted human teeth were inoculated with Enterococcus faecalis and divided into 6 groups (n=10), according to decontamination protocol: DW (control); 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl; 250 ppm HClO and 500 ppm HClO. The counting of colony-forming units evaluated the decontamination potential of each group, calculating the bacterial percentage reduction. Cytotoxicity test -Cytotoxicity was evaluated after inoculation of the same tested protocols in broblastic cells for 3 min, calculating the cell viability percentages. Speci cal statistical analysis was performed (α = 5%). Cleaning ability and erosion -Fifty-six single-rooted bovine lower incisors were divided into seven groups of 8 roots each, being the test groups 1% NaOCl; 2.5% NaOCl; 5,25% NaOCl; 250 ppm HClO and 500 ppm HClO, and a negative and positive control. Negative control was not contaminated, and the other groups were inoculated with Enterococcus faecalis. SEM images were ranked as from the cleanest to the least clean. Erosion was also assessed, being ranked from the least to the most eroded dentine.Results: The highest bacterial reduction was observed in experimental groups, with no statistical differences between them (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences between them (p < 0.05). 1% NaOCl; 2.5% NaOCl; 5.25% NaOCl and 500 ppm HClO displayed the cleanest areas. All sodium hypochlorite groups displayed erosion with higher ranks with greater concentration, while hypochlorous acid did not display any erosion regardless the concentration.Conclusions: It is possible to conclude that HClO obtained from an electrolytic device presented high antimicrobial activity and low cytotoxicity in both tested concentrations. 500 ppm HClO did not display erosion and showed great cleaning ability.Clinical relevance: The use of 500 ppm hypochlorous acid may reduce unfavorable behavior of sodium hypochlorite whilst maintaining its antimicrobial action.

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Lack of cytotoxicity by Trustwater Ecasol™ used to maintain good quality dental unit waterline output water in keratinocyte monolayer and reconstituted human oral epithelial tissue models

Cited by 18 publications

References 60 publications

Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells

Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells

Minimising microbial contamination in dental unit water systems and microbial control in dental hospitals

Antimicrobial action, cytotoxicity and erosive potential of hypochlorous acid obtained from an electrolytic device compared with sodium hypochlorite

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