Lack of Detection of Retroviral Particles (HIAP-1) in the H9 T Cell Line Co-Cultured with Thyrocytes of Graves' Disease

Fierabracci, Alessandra; Upton, Carol; Hajibagheri, Nasser; Bottazzo, Gian Franco

doi:10.1006/jaut.2001.0508

Cited by 17 publications

(5 citation statements)

References 39 publications

(47 reference statements)

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“…During this time, the few residual differentiated cells (e.g. thyrocytes, endothelial cells) remained attached to the bottom of the culture dish; thyrocytes were progressively de-differentiating into fibroblast-like cells, confirming a phenomenon known to occur between 7 and 10 days from the start of primary thyrocyte cultures (Fierabracci et al 2001). Meanwhile, small primary aggregates of cells appeared floating in the supernatant (Fig.…”

Section: Egf-and Bfgf-responsive Neurosphere-like Elements Derived Frsupporting

confidence: 59%

“…The blocks from all the thyroids were enzymatically digested in collagenase type IV (Gibco; 2 mg/ml in Roswell Park Memorial Institute Medium (RPMI); Fierabracci et al 2001); supernatants, containing the great majority of differentiated cells (e.g. thyrocytes, endothelial cells), were discarded, while thyroid fragments, residual to digestion, were collected, plated and cultured in the 'spheroid medium' in 100 mm polystyrene Petri culture dishes (BD Falcon, Becton Dickinson (BD), Franklin Lakes, NJ, USA).…”

Section: Primary Secondary and Higher-passaged Spheroid Culturesmentioning

confidence: 99%

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Identification of an adult stem/progenitor cell-like population in the human thyroid

Fierabracci¹,

Puglisi²,

Giuliani³

et al. 2008

Journal of Endocrinology

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There is evidence that tissue-specific stem cells reside in certain adult tissues. Their specific properties remain elusive, because they are rare and heterogeneous in parent tissues; furthermore, technical difficulties have been encountered in the identification and characterization of their progeny. The aim of this study was to isolate stem/progenitor cells from the human thyroid. We devised a method based on the enzymatic digestion of fresh surgical thyroid specimens, followed by culture of cells in the presence of epidermal growth factor and basic fibroblast growth factor. We also used markers that identify and characterize these cells. Spheroids with selfreplicative potential were obtained from all thyroid specimens. The isolated population contained a subset of CD34C CD45K cells and it was able, in differentiation conditions, to generate follicles with thyroid hormonal production. In support of the plasticity concept, we obtained evidence that, when most freshly isolated spheroids were co-cultured with a neuroblastoma cell line, they produced progeny expressing the neuronal marker b-tubulin III. Spheroids were also able to undergo adipogenic differentiation when cultured in adipogenic medium. We conclude that a predominant functional type of stem/progenitor cell exists within the thyroid, with an intrinsic ability to generate thyroidal cells and the potential to produce non-thyroidal cells.

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Section: Egf-and Bfgf-responsive Neurosphere-like Elements Derived Frsupporting

confidence: 59%

Section: Primary Secondary and Higher-passaged Spheroid Culturesmentioning

confidence: 99%

Identification of an adult stem/progenitor cell-like population in the human thyroid

Fierabracci¹,

Puglisi²,

Giuliani³

et al. 2008

Journal of Endocrinology

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“…However, no HIAP-1 particles are detected by electron microscopy in the H9 cells co-cultured with thyroid preparations of GD. These data call into question the involvement of HIAP-1 in the etiopathogenesis of Graves' disease [ 79 ].…”

Section: Introductionmentioning

confidence: 99%

Viruses and thyroiditis: an update

Desailloud

Hober

2009

Virol J

390

326

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Viral infections are frequently cited as a major environmental factor involved in subacute thyroiditis and autoimmune thyroid diseases This review examines the data related to the role of viruses in the development of thyroiditis.Our research has been focused on human data. We have reviewed virological data for each type of thyroiditis at different levels of evidence; epidemiological data, serological data or research on circulating viruses, direct evidence of thyroid tissue infection. Interpretation of epidemiological and serological data must be cautious as they don't prove that this pathogen is responsible for the disease. However, direct evidence of the presence of viruses or their components in the organ are available for retroviruses (HFV) and mumps in subacute thyroiditis, for retroviruses (HTLV-1, HFV, HIV and SV40) in Graves's disease and for HTLV-1, enterovirus, rubella, mumps virus, HSV, EBV and parvovirus in Hashimoto's thyroiditis. However, it remains to determine whether they are responsible for thyroid diseases or whether they are just innocent bystanders. Further studies are needed to clarify the relationship between viruses and thyroid diseases, in order to develop new strategies for prevention and/or treatment.

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“…While the genetic components have been well dissected, for example in T1D [29], we are still far from the identification of the environmental agents, including viruses, which may be responsible for triggering the disease process, or precipitating it [30]. Patients affected by autoimmune disorders are characterised by the production of several organ-and non-organ-specific autoantibodies.…”

Section: Discussionmentioning

confidence: 99%

Importin beta: A novel autoantigen in human autoimmunity identified by screening random peptide libraries on phage

Ola

Biro

Hawa

et al. 2006

Journal of Autoimmunity

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Lack of Detection of Retroviral Particles (HIAP-1) in the H9 T Cell Line Co-Cultured with Thyrocytes of Graves' Disease

Cited by 17 publications

References 39 publications

Identification of an adult stem/progenitor cell-like population in the human thyroid

Identification of an adult stem/progenitor cell-like population in the human thyroid

Viruses and thyroiditis: an update

Importin beta: A novel autoantigen in human autoimmunity identified by screening random peptide libraries on phage

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