Lack of Detection of the Mouse Mammary Tumor-like Virus (MMTV) Env Gene in Iranian Women Breast Cancer using Real Time PCR

Tabriz, Hedieh Moradi; Zendehdel, Kazem; Shahsiah, Reza; Fereidooni, Forouzandeh; Mehdipour, Baharak; Hosseini, Zahra

doi:10.7314/apjcp.2013.14.5.2945

Cited by 18 publications

(14 citation statements)

References 21 publications

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“…On the other hand, other studies conducted in England, Iran, Germany and Mexico, have never found the MMTV-like sequences in their breast cancer samples [ 30 , 12 – 14 ].…”

Section: Discussionmentioning

confidence: 90%

Detection of MMTV-Like sequences in Moroccan breast cancer cases

Slaoui

Mzibri

Rachid

et al. 2014

Infect Agents Cancer

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IntroductionThe mouse mammary tumor virus (MMTV) like sequences have been reported to be present in some human breast cancers, but their association with breast cancer development is still controversial.MethodsIn this retrospective study, we investigated the status of MMTV-like in 42 tumor biopsies and 18 paired normal tissues from Moroccan patients with breast cancer. MMTV-like env sequences were identified by PCR and confirmed by direct DNA sequencing.ResultsSpecific MMTV-like env sequences were found in 24 (57.14%) cases of breast carcinomas, and 6 (33.3%) cases of matched normal breast tissues. Comparison to sociologic and clinicopathological parameters showed no significant association between the presence of MMTV-like sequences and age, menopausal status, histological subtype, histological grade, tumor size and the expression of hormone receptors (estrogen ER and/or progesterone PgR) and Her 2. However, a significant correlation was found between MMTV-like presence and parity (p = 0.024).ConclusionsThis present study confirms the presence of MMTV-like env sequences in breast cancer in Moroccan women, prompting further evaluation, on large sampling, to elucidate the probable causal roles of MMTV-like in breast cancer development.

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“…On the other hand, other studies conducted in England, Iran, Germany and Mexico, have never found the MMTV-like sequences in their breast cancer samples [ 30 , 12 – 14 ].…”

Section: Discussionmentioning

confidence: 90%

Detection of MMTV-Like sequences in Moroccan breast cancer cases

Slaoui

Mzibri

Rachid

et al. 2014

Infect Agents Cancer

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“…The positivity ratio of MMTV-like virus detection in BC samples was varied population-wise from 0% (Oskouee et al 2014;Bindra et al 2007;Morales-Sanchez et al 2013;Motamedifar et al 2012;Park et al 2011;Tabriz et al 2013;Witt et al 2003) to 78.9% (Axel et al 1972) in all the 40 identified studies, while the positivity ratio of MMTV-like virus detection in normal and adjacent/ benign controls was varied from 0% (Oskouee et al 2014;Motamedifar et al 2012;Al Dossary et al 2018;Etkind et al 2000;Ford et al 2003;Lawson et al 2010;Mazzanti et al 2011;Naushad et al 2014;Reza et al 2015) to 32% (Glenn et al 2012) and 0% (Bindra et al 2007;Levine et al 1984;Melana et al 2002) to 33.3% (Slaoui et al 2014), respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Since then, numerous studies have been carried out worldwide for detecting MMTV-like virus in BC and their outcomes were contradictory because MMTV-like virus was detected in varying detection positivity ratios population-wide, i.e., from 0% (Oskouee et al (2014); Bindra et al 2007;Morales-Sanchez et al 2013;Motamedifar et al 2012;Park et al 2011;Tabriz et al 2013;Witt et al 2003) to 100% 78.9% (Axel et al 1972).…”

Section: Introductionmentioning

confidence: 99%

Does mouse mammary tumor-like virus cause human breast cancer? Applying Bradford Hill criteria postulates

2020

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Background The role of mouse mammary tumor-like virus (MMTV-like virus) in human breast cancer (BC) has already been widely investigated worldwide with conflicting results. Although the researchers tried to establish the link between MMTV-like virus and BC through the statistical meta-analysis of the previous studies associating MMTV-like virus with BC, they failed to establish a more reliable link due to the shortcomings of the statistical meta-analysis. In the present study, we identified population-wide studies relating MMTV-like virus with BC through the PubMed search engine. Then, we examined the available data of MMTV-like virus prevalence in BC, normal/benign samples, and applied the postulates of Bradford Hill criteria on the available evidence to investigate the association between MMTV-like virus and BC. In addition, to further enhance the reliability of our outcomes, we have also evaluated the methodologies of the previous studies to address the possibility of false-negative and false-positive results. Results After a careful evaluation of the extracted data against the postulates of Bradford Hill criteria, it was observed that none of the studies fulfill all the major postulates of Bradford Hill criteria for causation including temporality, consistency, biological gradient, experiment, coherence, specificity, and analogy. Hence, no causal relationship has been suggested between MMTV-like virus and BC patients of the any included population. Conclusion The results failed to prove the causal relationship between MMTV-like virus and BC rather suggested it as a co-participant in the pathogenesis of BC.

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“…The lack of MMTV-like sequences in our samples indicates that the concentration of these sequences is nil or very low in breast cancer tissue. Also, two other studies from Iran, in Tehran and Shiraz, have not found MMTV-like sequences in breast cancer specimens [16,17]. …”

Section: Discussionmentioning

confidence: 99%

No Evidence of Mammary Tumor Virus <b><i>env </i></b>Gene-Like Sequences among Iranian Women with Breast Cancer

Oskouee

Shahmahmoodi²,

Jalilvand³

et al. 2014

Intervirology

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Objective: The mouse mammary tumor virus (MMTV) is the well-established etiological agent of mammary tumors in mice. A series of studies have implicated that a human murine mammary tumor virus-like virus occurs in human breast cancer, but it is unclear whether it has any causal role. Methods: The aim of the present study was to investigate the presence of MMTV env gene-like sequences in a group of Iranian women with or without breast cancer. A total of 65 breast cancer and 65 noncancerous breast specimens from the Department of Pathology of Tabriz University in East Azerbaijan, Iran, were analyzed by nested PCR. Results: All breast cancer and benign breast samples were negative for MMTV env gene-like DNA. Conclusion: These results indicate that the MMTV env gene-like virus may not play a significant role in the etiology of breast cancer among Iranian women.

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Lack of Detection of the Mouse Mammary Tumor-like Virus (MMTV) Env Gene in Iranian Women Breast Cancer using Real Time PCR

Cited by 18 publications

References 21 publications

Detection of MMTV-Like sequences in Moroccan breast cancer cases

Detection of MMTV-Like sequences in Moroccan breast cancer cases

Does mouse mammary tumor-like virus cause human breast cancer? Applying Bradford Hill criteria postulates

No Evidence of Mammary Tumor Virus <b><i>env </i></b>Gene-Like Sequences among Iranian Women with Breast Cancer

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