Lack of detection of the Middle East respiratory syndrome coronavirus (MERS-CoV) nucleic acids in some Hyalomma dromedarii infesting some Camelus dromedary naturally infected with MERS-CoV

Hemida, Maged Gomaa; Alhammadi, Mohammed; Almathen, Faisal; Alnaeem, Abdelmohsen

doi:10.1186/s13104-021-05496-w

Cited by 6 publications

(7 citation statements)

References 42 publications

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“…For instance, they have been involved in the evolution of several human coronaviruses, but their exact role in the transmission of MERS-CoV remains unclear [ 53 ]. Hence, we screened our samples for this virus as well, including the collected ticks, although MERS-CoV infection does not involve a significant viremic period, and it has recently been shown that apparently neither ticks nor rodents play a role in the transmission cycle [ 53 , 54 ]. Concordant with the findings of Hemida et al, but in contrast to our findings with CCHFV [ 7 , 9 ], in this cohort we could not find any indications for the involvement of rodents or rodent-associated ticks in the transmission of CCHFV or MERS-CoV.…”

Section: Discussionmentioning

confidence: 99%

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Weidinger

Kolodziejek

Khafaga³

et al. 2023

Viruses

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The majority of emerging viral infectious diseases in humans originate from wildlife reservoirs, such as rodents and bats. We investigated a possible reservoir, namely wild gerbils and mice trapped in a desert reserve within the emirate of Dubai, United Arab Emirates (UAE). In total, 52 gerbils and 1 jird (Gerbillinae), 10 house mice (Mus musculus), and 1 Arabian spiny mouse (Acomys dimidiatus) were sampled. Oro-pharyngeal swabs, fecal samples, attached ticks, and organ samples (where available) were screened by (RT-q)PCR for the following viruses: Middle East respiratory syndrome-related coronavirus, Crimean-Congo hemorrhagic fever orthonairovirus, Alkhumra hemorrhagic fever virus, hantaviruses, Lymphocytic choriomeningitis mammarenavirus, Rustrela virus, poxviruses, flaviviruses, and herpesviruses. All of the samples were negative for all investigated viruses, except for herpesviruses: 19 gerbils (35.8%) and 7 house mice (70.0%) were positive. The resulting sequences were only partly identical to sequences in GenBank. Phylogenetic analysis revealed three novel betaherpesviruses and four novel gammaherpesviruses. Interestingly, species identification of the positive gerbils resulted in eight individuals clustering in a separate clade, most closely related to Dipodillus campestris, the North African gerbil, indicating either the expansion of the geographic range of this species, or the existence of a closely related, yet undiscovered species in the UAE. In conclusion, we could not find evidence of persistence or shedding of potentially zoonotic viruses in the investigated rodent cohorts of limited sample size.

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Section: Discussionmentioning

confidence: 99%

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Weidinger

Kolodziejek

Khafaga³

et al. 2023

Viruses

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Section: Discussionmentioning

confidence: 99%

“…Searching for some new unknown reservoir/s that may contribute to the sustainability and enhance the continuous transmission of the virus among dromedary camels is still one of the main research priorities in the epidemiology of MERS‐CoV. Some recent studies showed the lack of detection of MERS‐CoV‐RNAs in some species of ticks infesting some MERS‐CoV naturally infected camels (Hemida et al., 2021b). Another recent study reported the lack of detection of MERS‐CoV‐RNAs in samples from some species of rodents that shared their habitat with some index positive camel population (Hemida et al., 2021a).…”

Section: Discussionmentioning

confidence: 99%

“…However, neither the viral particles nor its nucleic acids were detected in the urine of infected camels (Farag et al., 2019). Meanwhile, recent studies reported the absence of any detectable viral nucleic acids of MERS‐CoV in some ticks infesting positive and actively shedding animals (Hemida et al., 2021b). Another recent study suggested that rodents sharing the habitats with an index MERS‐CoV naturally infected dromedary camel herd are unlikely to transmit the virus (Hemida et al., 2021a).…”

Section: Introductionmentioning

confidence: 99%

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Analyzing the roles of some species of arthropods in the transmission of the Middle East respiratory syndrome coronavirus

Hemida

Al‐Sabi

Alhammadi

et al. 2022

Veterinary Medicine & Sci

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Background The Middle East Respiratory Syndrome coronavirus (MERS‐CoV) is still listed on the WHO Research and Development Blueprint of emerging pathogens. Dromedary camels remain the only known animal reservoir of the virus. The animal‐to‐animal as well as the animal‐to‐human transmission in the MERS‐CoV cycles were reported. However, many aspects of these transmission chains are not well studied. One of these directions is the potential roles of various species of arthropods in the transmission of the virus. Objectives The main goal of the current work was to study the roles of several species of arthropods in the transmission of MERS‐CoV. Methodology To achieve this goal, we identified some MERS‐CoV naturally infected dromedary camel populations. We conducted a longitudinal study among these animals for more than 2 months. This was done by repeated testing of nasal swabs biweekly from some selected animals in this population for the presence of MERS‐CoV‐RNAs by real‐time PCR. During the duration of this study, we collected several species of arthropods (Culicoides, Stomoxys, Musca domestica and some Culex species) that shared the habitat and were circulating in this farm during this longitudinal study. Results Our results showing, despite the detection of the viral RNAs in some animals throughout this study, none of the examined species of arthropods tested positive for the viral RNA. Conclusions These results are suggesting that at least the tested species of arthropods may not play roles in the transmission of MERS‐CoV. However, more large‐scale studies are required to explore any potential roles of arthropods in the transmission cycle of MERS‐CoV.

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“…How I. scapularis ticks might become infected with SARS-CoV-2 is unclear. Based on a study of camels infected with Middle East Respiratory Syndrome coronavirus (MERS-CoV), the Hyalomma dromedarii ticks present on these animals did not demonstrate the presence of MERS-CoV [8]. Although multiple other viruses have been found in or on I. scapularis ticks collected before COVID-19 first appeared in the USA, none was a coronavirus [9].…”

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confidence: 99%

SARS-CoV-2 is Emerging in White-Tailed Deer and Can Infect and Spread Among Deer Mice Experimentally: What About Deer Ticks?

Bakshi

Centone²,

Wormser³

2022

The American Journal of Medicine

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Lack of detection of the Middle East respiratory syndrome coronavirus (MERS-CoV) nucleic acids in some Hyalomma dromedarii infesting some Camelus dromedary naturally infected with MERS-CoV

Cited by 6 publications

References 42 publications

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Analyzing the roles of some species of arthropods in the transmission of the Middle East respiratory syndrome coronavirus

SARS-CoV-2 is Emerging in White-Tailed Deer and Can Infect and Spread Among Deer Mice Experimentally: What About Deer Ticks?

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