Lack of Enhancement of Experimental Photocarcinogenesis by Retinoic Acid

Kligman, Lorraine H.; Kligman, A.M.

doi:10.1007/978-3-642-68023-6_61

Cited by 8 publications

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“…We have selected the SKH-1 mouse for these studies as it is a well accepted model system for studying skin barrier function, UVR induced skin cancer, and other diseases due to its similar follicular density and dermal to epidermal differentiation 38,39 . In previous studies, the biological effect of UVR irradiation has been examined at a variety of acute and repeated doses 40,41 .…”

Section: Introductionmentioning

confidence: 99%

“…We have selected the SKH-1 mouse for these studies as it is a well-accepted model system for studying skin barrier function, UVR induced skin cancer, and other diseases due to its similar follicular density and dermal to epidermal differentiation. , In previous studies, the biological effect of UVR irradiation has been examined at a variety of acute and repeated doses. , Here, we are interested primarily in acute UVR exposure, as it is the most applicable to consumer use and has been shown to have a significant effect on skin barrier function at a range of doses . To induce a level of UVR exposure that is similar to medium level sunburn in a human, a quantity of UVR (A and B) standardized to the UVB (270 mJ/cm 2 ) component is applied.…”

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confidence: 99%

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In Vivo Skin Penetration of Quantum Dot Nanoparticles in the Murine Model: The Effect of UVR

et al. 2008

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Ultraviolet radiation (UVR) has widespread effects on the biology and integrity of the skin barrier. Research on the mechanisms that drive these changes, as well as their effect on skin barrier function has been ongoing since the 1980s. However, no studies have examined the impact of UVR on nanoparticle skin penetration. Nanoparticles (NP) are commonly used in sunscreens and other cosmetics, and since consumer use of sunscreen is often applied to sun damaged skin, the effect of UVR on NP skin penetration is a concern due to potential toxicity. In this study we investigate nanoparticle skin penetration by employing an in vivo semiconductor quantum dot nanoparticle (QD) model system. This model system improves NP imaging capabilities and provides additional primary interest due to widespread and expanding use of QD in research applications and manufacturing. In our experiments, carboxylated QD were applied to the skin of SKH-1 mice in a glycerol vehicle with and without UVR exposure. The skin collection and penetration patterns were evaluated 8 and 24 hours after QD application using tissue histology, confocal microscopy, and transmission electron microscopy (TEM) and EDAX analysis. Low levels of penetration were seen in both the non-UVR exposed mice and the UVR exposed mice. Qualitatively higher levels of penetration were observable in the UVR exposed mice. These results are the first for in vivo QD skin penetration, and provide important insight into the ability of QD to penetrate intact and UVR compromised skin barrier. Our findings raise concern that NP of similar size and surface chemistry, such as metal oxide NP found in sunscreens, may also penetrate UV damaged skin.

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