Lack of genotoxicity of silver iodide in the SCE assay in vitro, in vivo, and in the Ames/microsome test

Eliopoulos, P.; Mourelatos, D.

doi:10.1002/(sici)1520-6866(1998)18:6<303::aid-tcm4>3.0.co;2-9

Cited by 7 publications

(6 citation statements)

References 7 publications

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“…Overall, silver ions do not indicate mutagenic activity in bacterial assays. An exception is silver iodide, exerting a minor effect in the TA97 Salmonella typhimurium frameshift strain (Eliopoulos and Mourelatos, 1998). Silver nanoparticles did not indicate any mutagenic activity in Salmonella typhimurium frameshift and base-pair substitution strains (Cho et al, 2013;Guo et al, 2016;H.…”

Section: Genotoxicity Studies In Vitromentioning

confidence: 87%

“…In bone marrow cells from mice intraperitoneally injected with silver nanoparticles, there were increased chromosomal aberrations but no increase in DNA strand breaks (Ghosh et al, 2012). Mice dosed by the same route with silver iodide showed no increase in sister chromatic exchanges in P388 lymphocyte leukemia cells (Eliopoulos and Mourelatos, 1998). In rats, inhalation of silver nanoparticles induced DNA…”

Section: Genotoxicity Studies In Vivomentioning

confidence: 99%

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Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: A review

Hadrup

Sharma

Loeschner

2018

Regulatory Toxicology and Pharmacology

255

178

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Silver is used in different applications that result in contact with skin and mucosal surfaces (e.g., jewelry, wound dressings, or eye drops). Intact skin poses an effective barrier against the absorption of silver. Mucosal surfaces are observed to be less effective barriers and compromised skin is often a poor barrier. Silver can deposit as particles in the human body causing a blue-gray discoloration known as argyria. Urine and feces are reported pathways of excretion. Acute human mortality has been observed following an abortion procedure involving the intrauterine administration of 7 g silver nitrate (64 mg silver/kg body weight). Localized argyria has been reported with exposure to silver ions, metallic surfaces, and nanocrystalline silver. Generalized argyria was observed with ionic and nanocrystalline silver in humans at cumulative doses in the range of 70-1500 mg silver/kg body weight. Silver is observed to have a low potential for skin irritation. Eye irritation and some cases of allergic contact dermatitis have been reported. Silver may cause genotoxicity, but additional data are required to assess its carcinogenic potential. Other reported toxicities include hepatic, renal, neurological, and hematological effects.

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Section: Genotoxicity Studies In Vitromentioning

confidence: 87%

Section: Genotoxicity Studies In Vivomentioning

confidence: 99%

Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: A review

Hadrup

Sharma

Loeschner

2018

Regulatory Toxicology and Pharmacology

255

178

View full text Add to dashboard Cite

show abstract

“…It is also worth noting that ionic silver is cytotoxic but not genotoxic in several different genotoxicity assays. Silver iodide was evaluated for mutagenicity in the Ames/ micro-some test and in the Sister Chromatid Exchanges assay, and was found to be negative in both assays even at toxic doses [Eliopoulos and Mourelatos, 1998]. Silver nitrate was also negative in the Drosophila wing somatic mutation assay while Ag-NPs were positive in this assay [Demir et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Silver nanoparticle‐induced mutations and oxidative stress in mouse lymphoma cells

Mei

Zhang

Chen

et al. 2012

Environ and Mol Mutagen

100

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Silver nanoparticles (Ag-NPs) have increasingly been used for coatings on various textiles and certain implants, for the treatment of wounds and burns, as a water disinfectant, and in air-freshener sprays. The wide use of Ag-NPs may have potential human health impacts. In this study, the mutagenicity of 5-nm Ag-NPs was evaluated in the mouse lymphoma assay system, and modes of action were assessed using standard alkaline and enzyme-modified Comet assays and gene expression analysis. Treatments of L5178Y/Tk(+/-) mouse lymphoma cells with 5-nm uncoated Ag-NPs resulted in a significant yield of mutants at doses between 3 and 6 μg/mL; the upper range was limited by toxicity. Loss of heterozygosity analysis of the Tk mutants revealed that treatments with uncoated Ag-NPs induced mainly chromosomal alterations spanning less than 34 megabase pairs on chromosome 11. Although no significant induction of DNA damage in Ag-NP-treated mouse lymphoma cells was observed in the standard Comet assay, the Ag-NP treatments induced a dose-responsive increase in oxidative DNA damage in the enzyme-modified Comet assay in which oxidative lesion-specific endonucleases were added. Gene expression analysis using an oxidative stress and antioxidant defense polymerase chain reaction (PCR) array showed that the expressions of 17 of the 59 genes on the arrays were altered in the cells treated with Ag-NPs. These genes are involved in production of reactive oxygen species, oxidative stress response, antioxidants, oxygen transporters, and DNA repair. These results suggest that 5 nm Ag-NPs are mutagenic in mouse lymphoma cells due to induction of oxidative stress by the Ag-NPs.

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“…Overall, the literature shows that genotoxicity data for Ag+ are limited. Ag+ is proven to be cytotoxic but did not induce genotoxicity in several different assays (Eliopoulos & Mourelatos, 1998;Demir et al, 2011). Ag iodide, for example, was not mutagenic in the Ames test and in the sister chromatid exchange assay (Eliopoulo & Mourelatos, 1998).…”

Section: Treatmentsmentioning

confidence: 99%

Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE)

Gasparoti

Gomes

Aleixo

et al. 2022

RSD

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The aim of the present study was to evaluate the cytotoxic potential of a new complex of silver(I) with furosemide (Ag-FSE) over a panel of tumor and non-tumor human cells, as well as its genotoxicological safety. According to the results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) was the most sensitive to the Ag-FSE complex, and among the non-tumor cells was the non-transforming fibroblasts known as GM07492A, which stimulated the characterization of cytotoxic properties against these cells. In addition to interfering with the cellular metabolic profile (evidenced by the resazurin method), Ag-FSE alters the integrity of cell membranes (results obtained by the Trypan blue assay) regardless of the cell line evaluated. However, interference with clonogenic capacity is dependent on the cell being tested; the cytotoxic activity evaluated by the clonogenic survival assay was evidenced against Caco-2 cells, but not against GM07492A cells. Furthermore, Ag-FSE did not induce an increase in the extent of DNA damage detectable by the comet assay, nor in the frequency of micronuclei compared to the negative control, demonstrating the absence of genotoxicity under the experimental conditions used. The data of the present study provide information on various biological aspects of the Ag-FSE complex and help in the generation of new drug candidates with potential to impact the health area, targeting cancer treatment.

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Lack of genotoxicity of silver iodide in the SCE assay in vitro, in vivo, and in the Ames/microsome test

Cited by 7 publications

References 7 publications

Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: A review

Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: A review

Silver nanoparticle‐induced mutations and oxidative stress in mouse lymphoma cells

Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE)

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