Lack of molecular evidence of fecal-borne viruses in capybaras from São Paulo state, Brazil, 2018–2020: a minor public health issue

Azevedo, Laís Sampaio de; França, Yasmin; Viana, Ellen; Medeiros, Roberta Salzone; Morillo, Simone Guadagnucci; Guiducci, Raquel; Ribeiro, Cibele Daniel; Vieira, Heloisa Rosa; Barrio-Nuevo, Karolina Morales; Cunha, Mariana Sequetin; Guerra, Juliana Mariotti; Silva, Dulcilena de Matos Castro e; Filho, Valter Batista Duo; Araújo, Emerson Luiz Lima; Ferreira, Sérgio Roberto Santos; Batista, Camila Freitas; Silva, Gislaine Celestino Dutra da; Nogueira, Maurício Lacerda; Ahagon, Cintia Mayumi; Moreira, Regina Célia; Cunha, Lia; Morais, Vanessa Santos; Costa, Antônio Charlys da; Luchs, Adriana

doi:10.1007/s42770-022-00859-2

Cited by 1 publication

(2 citation statements)

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“…Continuous monitoring of sylvatic animals is essential to prevent and control the emergence or re-emergence of newly discovered viruses as well as viruses with known zoonotic potential, especially in anthropogenic environments. Capybaras are extremely tolerant to environmental changes and exhibit high vagility along waterways, thus having potential to trigger emerging zoonosis [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…Controls for RT-qPCR inhibitors or for RNA extraction efficiency are not available for capybara origin specimens. Therefore, viability of capybaras stools screened in the present study was checked by MALDI-TOF MS and spiked with experimental specimens with rotavirus and adenovirus reference strains (representing enteric RNA and DNA viruses, respectively) as described elsewhere [ 52 ]. The WHO international standard for HEV NAT assays (Paul- Ehrlich-Institut code 6329/10, Paul-Ehrlich-Institut, Langen, Germany) was included in order to evaluate the analytical specificity of the RT-qPCR assay as well as serving as a positive control reaction.…”

Section: Methodsmentioning

confidence: 99%

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Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil

Cunha

Luchs

Azevedo

et al. 2023

Viruses

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Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in São Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7–93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%