Lack of mutagenicity of TiO2 nanoparticles in vitro despite cellular and nuclear uptake

Yamani, Naouale El; Rubio, Laura; García‐Rodríguez, Alba; Kažimı́rová, Alena; Rundén-Pran, Elise; Barančoková, Magdaléna; Marcos, Ricard; Dušinská, Maria

doi:10.1016/j.mrgentox.2022.503545

Cited by 5 publications

(4 citation statements)

References 50 publications

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“…In fact, E171 could be found inside the enterocytes as has been previously reported by other authors, 23 being able to reach the cellular nucleus (ref. 62 and Fig. 8I-J).…”

Section: Ultrastructure Of the Drosophila Intestine After Extended De...mentioning

confidence: 88%

“…39 Furthermore, nanomaterial internalization can reach the nucleus, where it can compromise DNA integrity. 39,60 We explored the possibility of subcellular alterations caused by E171 or E551 ingestion using TEM in intestines taken from larvae growing in 100 μg mL −1 E171 or E551 in the food media during 7 and 21 d. At the first time point of extended development control larvae show the characteristic epithelial ultrastructure described for the wild type Drosophila intestine 31,61 (Fig. 8D-F).…”

Section: Ultrastructure Of the Drosophila Intestine After Extended De...mentioning

confidence: 99%

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Complementary in vitro and in vivo strategies to assess the biological effects of the nano enabled food additives E171 and E551

Peropadre

Vega-Cuesta

Freire

et al. 2023

Environ. Sci.: Nano

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“…In fact, E171 could be found inside the enterocytes as has been previously reported by other authors, 23 being able to reach the cellular nucleus (ref. 62 and Fig. 8I-J).…”

Section: Ultrastructure Of the Drosophila Intestine After Extended De...mentioning

confidence: 88%

Section: Ultrastructure Of the Drosophila Intestine After Extended De...mentioning

confidence: 99%

Complementary in vitro and in vivo strategies to assess the biological effects of the nano enabled food additives E171 and E551

Peropadre

Vega-Cuesta

Freire

et al. 2023

Environ. Sci.: Nano

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“…The study employing transmission electron microscopy yielded evidence suggesting that the internalization of TiO 2 NPs by cells is observable within cytoplasmic vesicles and close to and inside the nucleus. Notably, larger agglomerates of TiO 2 NPs were believed to possess the capacity to disrupt or damage chromosomal structures, potentially leading to chromosome aberrations [62]. This meta-analysis incorporated the most recent studies of in vivo and in vitro genotoxicity and underwent rigorous quality assessments to enable quantitative analysis.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity Evaluation of Titanium Dioxide Nanoparticles In Vivo and In Vitro: A Meta-Analysis

Cao,

Chen,

Bian

et al. 2023

Toxics

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Background: Recent studies have raised concerns about genotoxic effects associated with titanium dioxide nanoparticles (TiO2 NPs), which are commonly used. This meta-analysis aims to investigate the potential genotoxicity of TiO2 NPs and explore influencing factors. Methods: This study systematically searched Chinese and English literature. The literature underwent quality evaluation, including reliability evaluation using the toxicological data reliability assessment method and relevance evaluation using routine evaluation forms. Meta-analysis and subgroup analyses were performed using R software, with the standardized mean difference (SMD) as the combined effect value. Results: A total of 26 studies met the inclusion criteria and passed the quality assessment. Meta-analysis results indicated that the SMD for each genotoxic endpoint was greater than 0. This finding implies a significant association between TiO2 NP treatment and DNA damage and chromosome damage both in vivo and in vitro and gene mutation in vitro. Subgroup analysis revealed that short-term exposure to TiO2 NPs increased DNA damage. Rats and cancer cells exhibited heightened susceptibility to DNA damage triggered by TiO2 NPs (p < 0.05). Conclusions: TiO2 NPs could induce genotoxicity, including DNA damage, chromosomal damage, and in vitro gene mutations. The mechanism of DNA damage response plays a key role in the genotoxicity induced by TiO2 NPs.

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“…Agglomerated nanoparticles may have different cellular interactions compared to individual nanoparticles. The concentration-dependent behavior could be related to changes in nanoparticle agglomeration and subsequent cellular uptake [70,71]. (4) Cell-specific responses: Different cell lines may exhibit varying sensitivities to TiO 2 -NPs due to differences in their inherent biological characteristics and signaling pathways [72,73].…”

Section: In Vitro Cytotoxicity Of Tio 2 -Npsmentioning

confidence: 99%

An Eco-Friendly Approach Utilizing Green Synthesized Titanium Dioxide Nanoparticles for Leather Conservation against a Fungal Strain, Penicillium expansum AL1, Involved in the Biodeterioration of a Historical Manuscript

Fouda

Abdel-Nasser²,

Eid

et al. 2023

Biology

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The main hypothesis of the present research is investigating the efficacy of titanium oxide nanoparticles (TiO2-NPs) to prevent the growth of fungal strains when applied on leather under an experimental study. Therefore, fifteen fungal strains were isolated from a deteriorated historical manuscript (papers and leathers) and identified by traditional methods and ITS sequence analysis, including Aspergillus chevalieri (one isolate), A. nidulans (two strains), A. flavus (four strains), A. cristatus (one strain), A. niger (one strain), Paecilomyces fulvus (two strains), Penicillium expansum (two strains), and P. citrinum (two strains). The enzymes cellulase, amylase, pectinase, and gelatinase, which play a crucial role in biodegradation, were highly active in these fungal strains. TiO2-NPs were formed using the cell-free filtrate of the probiotic bacterial strain, Lactobacillus plantarum, and characterized. Data showed that the TiO2-NPs were successfully formed with a spherical shape and anatase phase with sizes of 2–8 nm. Moreover, the EDX analysis revealed that the Ti and O ions occupied the main component with weight percentages of 41.66 and 31.76%, respectively. The in vitro cytotoxicity of TiO2-NPs toward two normal cell lines, WI38 and HFB4, showed a low toxicity effect against normal cells (IC50 = 114.1 ± 8.1µg mL–1 for Wi38, and 237.5 ± 3.5µg mL–1 for HFB4). Therefore, concentrations of 100 μg mL–1 were used to load on prepared leather samples before inoculation with fungal strain P. expansum AL1. The experimental study revealed that the loaded TiO2-NPs have the efficacy to inhibit fungal growth with percentages of 73.2 ± 2.5%, 84.2 ± 1.8%, and 88.8 ± 0.6% after 7, 14, and 21 days, respectively. Also, the analyses including SEM, FTIR-ART, color change, and mechanical properties for leather inoculated with fungal strain AL1 in the absence of NPs showed high damage aspects compared to those inoculated with fungal strains in the presence of TiO2-NPs.

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Lack of mutagenicity of TiO2 nanoparticles in vitro despite cellular and nuclear uptake

Cited by 5 publications

References 50 publications

Complementary in vitro and in vivo strategies to assess the biological effects of the nano enabled food additives E171 and E551

Complementary in vitro and in vivo strategies to assess the biological effects of the nano enabled food additives E171 and E551

Genotoxicity Evaluation of Titanium Dioxide Nanoparticles In Vivo and In Vitro: A Meta-Analysis

An Eco-Friendly Approach Utilizing Green Synthesized Titanium Dioxide Nanoparticles for Leather Conservation against a Fungal Strain, Penicillium expansum AL1, Involved in the Biodeterioration of a Historical Manuscript

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