Lack of neuropeptide FF signalling in mice leads to reduced repetitive behavior, altered drinking behavior, and fuel type selection

Abstract

layer of the superior colliculus; uCT, micro-computed tomography; VCO 2 , carbon dioxide production; VDB, nucleus of the vertical limb of the diagonal band; VO 2 , oxygen consumption; WT, wildtype.

Central NPFF signalling is critical in the regulation of glucose homeostasis

“…Both, NPFF and NPAF act preferentially on the NPFFR2 receptor [ [3] , [4] , [5] ]. Originally identified as modulators of morphine-induced analgesia [ 6 ], NPFF group peptides and their receptor have since been shown to take part in other physiological functions, such as fluid homeostasis, energy metabolism and anxiety-like behaviours [ [7] , [8] , [9] , [10] , [11] , [12] ]. The expression of Npff is restricted to the caudal brainstem and spinal cord in the mouse central nervous system [ 2 , 11 ].…”

“…Originally identified as modulators of morphine-induced analgesia [ 6 ], NPFF group peptides and their receptor have since been shown to take part in other physiological functions, such as fluid homeostasis, energy metabolism and anxiety-like behaviours [ [7] , [8] , [9] , [10] , [11] , [12] ]. The expression of Npff is restricted to the caudal brainstem and spinal cord in the mouse central nervous system [ 2 , 11 ]. In the caudal brainstem, Npff is predominantly expressed in the subpostrema area (SubP), – the part of the nucleus of tractus solitarius (NTS) immediately ventral to the area postrema (AP) [ 2 , 7 , 11 ].…”

“…The expression of Npff is restricted to the caudal brainstem and spinal cord in the mouse central nervous system [ 2 , 11 ]. In the caudal brainstem, Npff is predominantly expressed in the subpostrema area (SubP), – the part of the nucleus of tractus solitarius (NTS) immediately ventral to the area postrema (AP) [ 2 , 7 , 11 ]. The Npff mRNA levels are influenced by energy status with negative energy balance induced by fasting leading to a strong increase in expression, while positive energy balance induced by high fat feeding has the opposite effect [ 7 ], implicating NPFF peptides in energy homeostasis control.…”

“…The Npff mRNA levels are influenced by energy status with negative energy balance induced by fasting leading to a strong increase in expression, while positive energy balance induced by high fat feeding has the opposite effect [ 7 ], implicating NPFF peptides in energy homeostasis control. Recently, it was shown that lack of NPFF signalling in mice leads to an altered oxidative fuel type selection during a fasting-refeeding challenge, while having little impact on feeding [ 11 ]. In comparison to NPFF, its high affinity receptor NPFFR2, shows a far wider expression throughout the brain; however also has a prominent expression in the dorsal motor nucleus of the vagus (DMV) [ 11 , 13 ].…”

“…Recently, it was shown that lack of NPFF signalling in mice leads to an altered oxidative fuel type selection during a fasting-refeeding challenge, while having little impact on feeding [ 11 ]. In comparison to NPFF, its high affinity receptor NPFFR2, shows a far wider expression throughout the brain; however also has a prominent expression in the dorsal motor nucleus of the vagus (DMV) [ 11 , 13 ]. The AP, NTS and DMV form the dorsal vagal complex (DVC) – an autonomic regulatory centre containing preganglionic parasympathetic neurons that control the subdiaphragmatic viscera [ 14 , 15 ].…”