Lack of occult hepatitis B virus infection among blood donors with isolated hepatitis B core antibody living in an HBV low prevalence region of Iran

Sofian, Masoomeh; Aghakhani, Arezoo; Izadi, Nabiallah; Banifazl, Mohammad; Kalantar, Enayatollah; Eslamifar, Ali; Ramezani, Amitis

doi:10.1016/j.ijid.2009.05.011

Cited by 39 publications

(34 citation statements)

References 35 publications

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“…reported that 2.1% of blood donors were HBcAb positive. They did not detect any viral DNA in the population under study (20). The authors noted that Arak province is a low prevalence region for HBV infection.…”

Section: Discussionmentioning

confidence: 90%

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

et al. 2013

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BackgroundPresence of occult hepatitis B infection (OBI) renders HBs antigen (HBsAg) undetectable by ELISA. Therefore it is valuable to evaluate the frequency of OBI among healthy blood donors to improve and perhaps change the strategies of blood screening to reduce the risk of HBV transmission.ObjectivesThe aim of this study was to determine the presence of HBcAb and HBV DNA among Iranian HBsAg negative healthy blood donors who donated their blood to the Tehran Blood Transfusion Center during 2011.Patients and Methods1000 serum specimens negative for HBsAg, HCV antibody and HIV antibody were collected from healthy blood donors and tested for HBcAb. Presence of hepatitis B viral DNA was checked in HBcAb positive samples by nested PCR with two sets of primers to amplify part of HBV S gene.ResultsThere were 64 women and 936 men in the population under study. The mean ± SD age of the donors was 38 ± 11 years. 80 out of 1000 samples (8%) were found to be positive for HBcAb. HBV DNA was detected in 50% of HBcAb positive specimens. The mean ± SD age of donors without HBV DNA was 37.7 ± 10.5 years and for donors with HBV DNA was 40.9 ± 11.2 years (P = 0.05).ConclusionsOBI was prevalent among 50% of HBcAb positive healthy blood donors. The frequency of positive HBcAb among healthy HBsAg negative blood donors was comparable to previous studies reported from Iran. On the other hand, the frequency of HBV DNA in HBsAg negative blood donors was higher than previous reports.

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Section: Discussionmentioning

confidence: 90%

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

et al. 2013

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“…Various reports are available on different population types that have been conducted in Iran. Most of them focused on high‐risk populations and showed varied results: 0% in blood donors, 22% among drug users, and 30% among hemodialysis and HIV‐positive people . Corresponding data is inadequate across anti‐HBc positive cases; 2.72% out of 110 patients in Zahedan city, 28.57% out of just 14 anti‐HBc positive cases in Isfahan, and in Tehran HBV‐DNA was detected among 12.2% out of 131 anti‐HBc samples…”

Section: Discussionmentioning

confidence: 99%

Epidemiology, risk factors, and molecular characterization of occult hepatitis B infection among anti‐hepatitis B core antigen alone subjects

Azarkar

Ziaee

Ebrahimzadeh

et al. 2018

Journal of Medical Virology

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Objectives Features of occult hepatitis B virus (HBV) infection among the anti‐hepatitis B core antigen (anti‐HBc) positives have yet to be described in more details. This study aimed to determine the molecular prevalence of occult HBV infection (OBI), and association to risk factors among seropositives for anti‐HBc. Methods This was part of a community‐based screening project that included 5234 cases. All participants completed a questionnaire on demographic and socio‐epidemiological information. Then, the blood samples were collected and tested for anti‐HBc and HBsAg using ELISA method. To identify OBI, nested‐polymerase chain reaction (PCR) assays were performed for HBV‐S and X genes, and viral load was determined using an in‐house real‐time PCR. Sequencing and phylogenetic analysis have been implemented for genotyping. Results Overall, 596 cases, positive only for anti‐HBc were included in the study. OBI was detected among 61 cases (10.2%). The genotype and subgenotype of HBV among all of them was D1, except one that was D4. Most of them had low viral loads ranged from 1.2 × 102 to 1.34 × 10 3 copies/mL; 19.6% had undetectable viral loads. Important mutations in surface protein and reverse transcriptase were sI92T, sQ129H, rtL80I, rtS85F, rtL91I. The prevalence of OBI was related to some risk factors, such as tattooing (P = 0.02), sexual activities (P = 0.009), and diabetes (P = 0.031). Conclusion Our study suggests that OBI should be considered among anti‐HBc seropositive subjects. This form of HBV infection was accompanied with some mutations, risk factors, and diseases. However, further investigations are needed to determine virological importance of documented mutations.

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“…In a study by Salawu et al in Ile Ife, Nigeria, a prevalence of 4.4% was reported for anti-HBc. 32 Ramezani et al 33 and Sofian et al 34 both reported prevalence rates of 2.1%, but without detection of HBV DNA. A serological pattern of anti-HBc as a sole marker is not infrequent.…”

Section: 30mentioning

confidence: 97%

Prevalence of antibody to hepatitis B core antigen among hepatitis B surface antigen-negative blood donors in Ilorin, Nigeria: A cross-sectional study

Ogunfemi¹,

Olawumi²,

Olokoba³

et al. 2017

Mal. Med. J

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BackgroundPost-transfusion hepatitis occurs even with stringent donor selection criteria and screening for hepatitis B surface antigen (HBsAg). The objective of this study was to determine the prevalence of antibody to hepatitis B core antigen (anti-HBc) in HBsAg-negative blood donors. MethodsThis was a cross-sectional study in which 200 HBsAg-negative blood donors were recruited. Screening for viral markers was done using both a rapid test kit and enzyme-linked immunosorbent assay (ELISA) for anti-HBc IgM. Quantitative and qualitative analysis of anti-HBc IgM was done by "capture" enzyme immunoassay using DIA.PRO HBc IgM test kits. The other viral markers were investigated using one step cassette style HBV tests. SPSS version 16 was used for data analysis. A P-value of 0.05 or less was considered significant. ResultsThere were 190 male (95%) and 10 female (5%) blood donors, with a mean age of 31.7 ± 7.9 years. The prevalence of anti-HBc IgM was 4%. The other viral markers (HBeAg, anti-HBeAg, anti-HBs and total anti-HBc) had a prevalence of 1.5%, 23%, 2.5%, and 32.5%, respectively.

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Lack of occult hepatitis B virus infection among blood donors with isolated hepatitis B core antibody living in an HBV low prevalence region of Iran

Abstract: Our study showed that all the blood donors with isolated anti-HBc were negative for HBV-DNA, and occult HBV infection did not occur in the blood donors of this low prevalence region for HBV infection.

Cited by 39 publications

References 35 publications

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

Hepatitis B Viral DNA Among HBs Antigen Negative Healthy Blood Donors

Epidemiology, risk factors, and molecular characterization of occult hepatitis B infection among anti‐hepatitis B core antigen alone subjects

Prevalence of antibody to hepatitis B core antigen among hepatitis B surface antigen-negative blood donors in Ilorin, Nigeria: A cross-sectional study

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