Lack of papillomavirus (HPV) in pterygia of a Brazilian sample

Schellini, Silvana Artioli; Hoyama, Érika; Shiratori, Cláudia Akemi; Sakamoto, Regina Hitomi; Candeias, J. A. N.

doi:10.1590/s0004-27492006000400012

Cited by 13 publications

(12 citation statements)

References 10 publications

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“…Similar results were reported by Dushku et al. (1999), who examined populations in the USA, and Schellini et al. (2006), who conducted their study in Brazil.…”

Section: Discussionsupporting

confidence: 87%

“…Chen et al (2003), in their study conducted in Taiwan, did not identify HPV DNA in pterygium patients by PCR. Similar results were reported by Dushku et al (1999), who examined populations in the USA, and Schellini et al (2006), who conducted their study in Brazil. The differences between these observations and our findings may result from geographical differences in the incidence of HPV.…”

Section: Discussionsupporting

confidence: 87%

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Occurrence of human papillomavirus in pterygia

Piecyk-Sidor

Polz‐Dacewicz

Zagórski

et al. 2009

Acta Ophthalmologica

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ABSTRACT.Purpose: The aim of the study was to assess the occurrence of human papillomavirus (HPV) DNA in pterygium. Methods: The study involved 89 patients undergoing surgical procedures at the Department of Ophthalmology, Medical University of Lublin, Poland. Group 1 included 58 patients with clinically diagnosed pterygium. Group 2 consisted of 31 individuals with normal conjunctiva. The material was collected during elective surgical procedures. The presence of HPV genome was determined using polymerase chain reaction (PCR). Once the presence of HPV DNA was confirmed, 28 HPV genotypes were determined using reverse hybridization. Results: The determinations confirmed the presence of HPV DNA in pterygium. In the material collected from 58 cases of pterygium (group 1), HPV DNA was identified in 16 patients (27.6%). In the material from 31 diagnostic specimens of normal conjunctiva (group 2), the presence of HPV was demonstrated in three cases (9.7%). A statistically significant difference was found in the presence of HPV DNA between the patients from groups 1 and 2 (p = 0.041). HPV type 16 was most common and was demonstrated in 56% of HPV-positive cases of pterygium. HPV 16 and HPV 6 co-infections were found in 19% of cases, while HPV 18 and HPV 6 co-infections were observed in 13%. In group 2, all three patients with HPV showed HPV 18. Conclusion: It seems that HPV is not necessary to induce pterygium; however, it might play a synergistic role in the multi-stage process of its development.

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“…Similar results were reported by Dushku et al. (1999), who examined populations in the USA, and Schellini et al. (2006), who conducted their study in Brazil.…”

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 87%

Occurrence of human papillomavirus in pterygia

Piecyk-Sidor

Polz‐Dacewicz

Zagórski

et al. 2009

Acta Ophthalmologica

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“…Although in a study conducted in Brazil, none of the 36 samples tested by the authors was positive for HPV, the authors had not outlined clearly the HPV detection method that was employed (Schellini et al. ). In another report on HPV in pterygia, the researchers found a very low prevalence of 4.4%, all of which were of the low‐risk HPV type 6 (Sjö et al.…”

Section: Discussionmentioning

confidence: 97%

Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi‐ethnic patients in the Malay Peninsula

Chong

Tung

Rahman

et al. 2014

Acta Ophthalmologica

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ABSTRACT.Purpose: The aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula. Methods: DNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 typespecific TaqMan probes was employed to determine the viral DNA copy number. Results: Of 45 pterygia samples with acceptable DNA quality, 29 (64.4%) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4% of positive samples) genotype followed by type 16 (27.6%). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 3 10 2 and 3 3 10 4 copies per lg, whereas the viral load of HPV16 specimen was 4 3 10 1 to 10 2 copies per lg. Conclusion: This report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.

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“…Of note, certain studies (such as those of Sjö et al from Denmark, Takamura et al from Japan or Hsiao et al from Taiwan) have failed to detect HPV or report very low prevalences of HPV in examined pterygia (21–23,25,27). Moreover, Dushku et al detected p53 overexpression in the limbal epithelium of the pterygia studied with all the samples being negative for HPV DNA, suggesting that factors other than HPV infection were responsible for the p53 overexpression (12).…”

Section: Current Status On Hpv Detection In Pterygiummentioning

confidence: 99%

Viral involvement in the pathogenesis and clinical features of ophthalmic pterygium (Review)

Chalkia

Spandidos

Detorakis

2013

International Journal of Molecular Medicine

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Pterygium is a potentially vision-threatening fibrovascular lesion originating from the conjunctiva that often extends on the corneal surface. Although it has been extensively studied, its pathogenesis has yet to be fully elucidated. Recent evidence on molecular genetic abnormalities in pterygium suggested neoplastic changes of limbal stem cells potentially associated with exposure to ultraviolet (UV) light. Human papillomavirus (HPV) is an oncogenic virus, associated with squamo-proliferative lesions of the anogenital region, skin and oropharynx. Several studies have shown HPV involvement in the pathogenesis of conjunctival neoplastic lesions, including papilloma and squamous cell carcinoma. The involvement of HPV as a co-factor in the pathogenesis of pterygium, although suggested by several studies using PCR and immunohistochemical techniques, remains controversial. Moreover, a marked variation in the prevalence of HPV in ophthalmic pterygium has been reported by different studies. Ethnic susceptibility and methodological differences in the detection of HPV may account for this variation. Surgical excision, often using sophisticated techniques, is the standard current method of therapy for pterygium. However, recurrences are frequent and recurrent lesions tend to be more aggressive. If indeed HPV is involved in pterygium pathogenesis or recurrence, anti-viral medications or vaccination may be new options in pterygium therapy.

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Lack of papillomavirus (HPV) in pterygia of a Brazilian sample

Cited by 13 publications

References 10 publications

Occurrence of human papillomavirus in pterygia

Occurrence of human papillomavirus in pterygia

Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi‐ethnic patients in the Malay Peninsula

Viral involvement in the pathogenesis and clinical features of ophthalmic pterygium (Review)

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