Lack of protective effect of autotransplanted splenic tissue to pneumococcal challenge

Schwartz, Allen D.; Goldthorn, JF; Winkelstein, JA; Swift, AJ

doi:10.1182/blood.v51.3.475.bloodjournal513475

Cited by 9 publications

(14 citation statements)

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“…Loss of the spleen carries a risk of overwhelming postsplenectomy infection by encapsulated bacteria (Llende, 1986). Splenules in the rat either failed to pro- tect (Schwartz et al, 1978;Scher et al, 19851, or only partially protected (Livingston et al, 1983a;Moxon and Schwartz, 19801, against bacterial infection. The quantity of splenic tissue necessary to attain normal splenic function has been studied in animals with splenules.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopic study of subcutaneous and intraperitoneal splenules in the mouse

Johnson

Weiss

1989

Am. J. Anat.

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The development of splenules derived from slices of freshly removed autologous spleen implanted subcutaneously or intraperitoneally was followed by light and electron microscopy from day 2 to day 70. Within 48 hr after transplantation, a rough space filled with blood, unlined by endothelium, formed just under the surface of the splenic fragment. The tissue central to this vascular space was disrupted and necrotic. In the outer portion of the vascular space, fibroblasts appeared and created locules which developed into a highly vascular, hematopoietic red pulp. From the inner portion, blood percolated into the central necrotic tissue. At 1 week the splenule was divisible into concentric structures. The capsule was outermost. A shell of vascularized, highly hematopoietic red pulp lay within the capsule, having replaced the vascular space. Central to the red pulp lay a band of fibroblasts and macrophages. Next was a layer of fibroblasts in a matrix of degenerating cells, and, at the center, a necrotic core. As fibroblasts and macrophages moved centrad, the red pulp moved with them, expanding and replacing the necrotic tissue. The splenule differed in character from the original spleen. Splenular red pulp, especially near the surface, was unusually hematopoietic. The circumferential reticulum of white pulp was reduced or absent, and the boundary between red and white pulp was sometimes indistinct. Some white pulp was subcapsular, and the capsule and surrounding connective tissue were infiltrated by lymphocytes. The necrotic core of the splenule was typically surrounded by a zone containing large blood vessels, connective tissue, and adipocytes.

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Section: Discussionmentioning

confidence: 99%

Electron microscopic study of subcutaneous and intraperitoneal splenules in the mouse

Johnson

Weiss

1989

Am. J. Anat.

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“…In animal models, splenic tissue implants can restore some immunological activities (Likhite, 1975) and in man inadvertently autotransplanted tissue may function (Mazur, Field, Cahow, Schiffman, Duffy & Forget, 1978) and be responsible for the low incidence of infection after trauma. However, autotransplanted splenic tissue does not protect from pneumococcal challenge in rats (Schwartz, Goldthorn, Winkelstein & Swift, 1978).…”

Section: Possibility Of Autotransplantationmentioning

confidence: 93%

Consequences of Impaired Splenic Function

Bullen

Losowsky

1979

Clinical Science

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“…Experimental studies investigating the immunoprotector effect of autogenous splenic implants are based on animal exposure to several bacterial species, with evaluation of the following factors: bacterial clearance from the bloodstream (Patel et al, 1986;Cooney et al, 1979;Brown et al, 1981;Malangoni et al, 1988), mortality due to the sepsis caused by the microorganisms (Cooney et al, 1979;Schwartz et al, 1978;Livingston et al, 1982;Malangoni et al, 1985), as well as the potential benefits of immunization combined with splenic autotransplantation (Leemans et al, 1999;Cooney et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

Bacterial phagocytosis by macrophage of autogenous splenic implant

2003

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Autogenous splenic implant seems to be the only alternative for preservation of splenic tissue after total splenectomy. This work was carried out to analyze the morphologic regeneration of autotransplanted splenic tissue in Wistar rats and to determine the bacterial phagocytic function of their macrophages. We utilized an experimental model with thirty-two rats, of both sexes, submitted to total splenectomy combined with autotransplantation in greater omentum of slices of the whole spleen mass. The animals were divided into two groups: I--young rats weighing 100 to 150 g; and II--adult rats weighing 250 to 300 g. Sixteen weeks later animals were intravenously inoculated with a suspension of Escherichia coli AB1157. Twenty minutes after inoculation, the animals were sacrificed and the splenic autotransplants were removed for morphological study. There was regeneration of autotransplanted splenic tissue in all animals. A similar morphological aspect among all animals was observed, with splenic tissue showing red and white pulps, lymphoid follicles, and marginal zone, with a moderate architectural disarrangement. Macrophages containing gram-negative bacterial aggregates as well as macrophages with hemosiderin pigments within the cytoplasm were observed. Blood vessels showed preserved walls, with no signs of vasculitis or thrombosis. The present results suggest that autogenous splenic implants in the greater omentum of the rat acquire the macro- and microscopic architecture of a normal spleen, with reduced dimensions, and preserve bacterial phagocyte function.

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Lack of protective effect of autotransplanted splenic tissue to pneumococcal challenge

Cited by 9 publications

References 0 publications

Electron microscopic study of subcutaneous and intraperitoneal splenules in the mouse

Electron microscopic study of subcutaneous and intraperitoneal splenules in the mouse

Consequences of Impaired Splenic Function

Bacterial phagocytosis by macrophage of autogenous splenic implant

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