Lack of renal effects of DOCA, ACTH, spironolactone, and angiotensin II in <i>Squalus acanthias</i>

Churchill, Paul C.; Malvin, Richard L.; Churchill, Monique C.

doi:10.1002/jez.1402340104

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…McCormick et al [30] investigated the effects of spironolactone on mineralocorticoid functions in Atlantic salmon. Churchill et al [31] investigated its effects on renal function in spiny dogfish shark (Squalus acanthias). However, none of the studies to date have reported masculinization effects in response to SPL treatment in fish.…”

Section: Effects Of Spironolactone On Female Mosquitofishmentioning

confidence: 99%

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Endocrine-disrupting effects of spironolactone in female western mosquitofish, Gambusia affinis

Raut

Howell

Angus

2011

Environmental Toxicology and Chemistry

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The discovery of pharmaceuticals in effluent from wastewater treatment plants and drug manufacturing facilities and in receiving waters has raised environmental concern. Because these compounds are ending up in the environment, it is important to investigate the effects of these compounds on wildlife as well as humans. The present study used a fish model to investigate the endocrine-disrupting effects of spironolactone (SPL), an aldosterone antagonist used as a diuretic, but which also exhibits antiandrogenic effects in humans. A dose-response study measured the effects of SPL on anal fin ray elongation, an androgen-dependent secondary sex trait, and expression of the vitellogenin gene, an estrogen-dependent trait, in female western mosquitofish, Gambusia affinis. Fish were exposed to SPL in the water for 35 d at four nominal concentrations: 10, 100, 250, and 500 nM (4.2, 41.7, 104.1, and 208.3 µg/L, respectively) via the static renewal method. Masculinization of females, as evidenced by development of an elongated and modified anal fin, was observed in the fish exposed to the three highest concentrations. Anal fin elongation was observed in the group exposed to the lowest SPL concentration, but without the development of a tip apparatus. These results confirm the results of a preliminary study that, in contrast to antiandrogenic effects seen in humans, SPL has androgenic and/or antiestrogenic activity in a fish.

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Section: Effects Of Spironolactone On Female Mosquitofishmentioning

confidence: 99%

“…Alarmingly, treatment of wastewater from pharmaceutical manufacturing plants does not always appear to effectively remove their products from the effluent. Pharmaceuticals have been measured in wastewater effluent from pharmaceutical manufacturing plants in concentrations ranging from less than 1 ng/L to as high as 31,000 mg/L [5].…”

Section: Introductionmentioning

confidence: 99%

Endocrine-disrupting effects of spironolactone in female western mosquitofish, Gambusia affinis

Raut

Howell

Angus

2011

Environmental Toxicology and Chemistry

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“…In addition, fish kidneys are very different from those of mammals; they have a higher filtration rate and different filtration, secretion, and reabsorption selectivity [ 36 ]. These differences can influence the elimination rates of the drugs such as meloxicam, which is partly eliminated in urine [ 37 ]. Previous studies also revealed the following kidney differences between freshwater and marine fish: marine species have generally smaller and fewer glomeruli, together with lower filtration rates to those of freshwater fish species [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of the Anti-Inflammatory Drug Meloxicam after Single 1.5 mg/kg Intramuscular Administration to Undulate Skates (Raja undulata)

Morón-Elorza

Cañizares-Cooz

Rojo-Solís³

et al. 2022

Veterinary Sciences

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The therapy database currently used in elasmobranchs is still mostly based on empirical data, and there are few efficacy and safety studies supporting clinical practice. In this study, meloxicam pharmacokinetics (PK) were evaluated after a single 1.5 mg/kg IM administration to a group of seven clinically healthy adult undulate skates (Raja undulata Lacepède, 1802). Blood samples were collected before administration and at 15, 30, 60 and 90 min and 2, 4, 8, 12, 24 and 48 h after the IM injection. The meloxicam concentrations in plasma were determined using high-performance liquid chromatography, and PK parameters were calculated using a non-compartmental model approach. The mean ± SEM values of the main PK values were 1.84 ± 0.31 μg/mL for peak plasma concentration, 1.5 ± 0.24 h for time to maximum plasma concentration, 11.43 ± 2.04 h·µg/mL for area under the plasma concentration vs. time curve, 3.55 ± 0.65 h for elimination half-life, and 5.37 ± 0.94 h for mean residency time. No adverse reactions were detected. The relatively high plasma concentration and short time to maximum plasma concentration suggest that meloxicam could turn into an efficient analgesic and anti-inflammatory candidate drug to be used in skates. Further efficacy, pharmacodynamic, and multiple-dose studies with meloxicam are needed in elasmobranchs.

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Stage‐related production of 21‐hydroxylated progestins by the dogfish (Squalus acanthias) testis

Barry

Thomas²,

Callard

1993

J. Exp. Zool.

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Using shark (Squalus acanthias) testicular microsomes and [3H]progesterone ([3H]P) and [3H]17 alpha-hydroxyprogesterone ([3H]17 alpha-P) as substrates, two major products of 21-hydroxylase action were identified; respectively, 21-hydroxy-4-pregnen-3-one (11-deoxycorticosterone, DOC) and 17 alpha,21-dihydroxy-4-pregnen-3-one (11-deoxycortisol,S). Additional products of 17 alpha-hydroxylase/C-17,20-lyase action were detected: 17 alpha-hydroxyprogesterone (17 alpha P), testosterone (T), and androstenedione (AE). When microsomes derived from tissues in premeiotic (PrM), meiotic (M), and postmeiotic (PoM) stages of spermatogenesis were compared, maturation-related increases were observed with both 21-hydroxylase (3- to 20-fold) and 17 alpha-hydroxylase (2- to 6-fold). With [3H]P as tracer, the half-maximal substrate concentrations (Km = 0.2-1.0 microM) and maximal reaction velocities (Vmax = 9-25 pmol/mg protein/min) were similar for both enzymes when assayed in the same preparation, suggesting they compete for available substrate. Also, the presence of 1- or 10-fold molar excess radioinert DOC reduced conversion of [3H]P to 17 alpha-hydroxylated products. [3H]DOC itself was a substrate of 17 alpha-hydroxylation but not C-17,20-lyase action. Expression of 21-hydroxylase and 17 alpha-hydroxylase activities in cultured spermatocysts (intact germ cell/Sertoli cell units) was confirmed by detection of immunoreactive 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S),S,17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-P), DOC, P and T in spent media. 20 beta-S and S secretion increased and 17 alpha,20 beta-P decreased progressively with stage of maturation, but DOC was similar in all stages. P secretion was maximal and T lowest in M-stage spermatocysts, but when DOC (0.1 microM) was added to PrM or PoM spermatocysts, T output decreased. Taken together, the data suggest that 21-hydroxylating pathways, via direct and indirect mechanisms, affect accumulation of bioactive steroids (P and T) differentially by stage of spermatogenic development. Whether 21-hydroxylated progestins produced in shark testis have paracrine or endocrine actions in their own right remains to be investigated.

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Lack of renal effects of DOCA, ACTH, spironolactone, and angiotensin II in Squalus acanthias

Cited by 5 publications

References 21 publications

Endocrine-disrupting effects of spironolactone in female western mosquitofish, Gambusia affinis

Endocrine-disrupting effects of spironolactone in female western mosquitofish, Gambusia affinis

Pharmacokinetics of the Anti-Inflammatory Drug Meloxicam after Single 1.5 mg/kg Intramuscular Administration to Undulate Skates (Raja undulata)

Stage‐related production of 21‐hydroxylated progestins by the dogfish (Squalus acanthias) testis

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