Lack of resistance to macrolides in Mycoplasma genitalium detected in South African pregnant women

Naicker, Meleshni; Singh, Ravesh; Westhuizen, Donald van der; Tinarwo, Partson; Abbai, Nathlee

doi:10.4102/sajid.v36i1.209

Cited by 3 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22 A recent study conducted among pregnant women in KwaZulu-Natal, South Africa reported a prevalence of 5.9% for M. genitalium . 23…”

Section: Discussionmentioning

confidence: 99%

“…22 A recent study conducted among pregnant women in KwaZulu-Natal, South Africa reported a prevalence of 5.9% for M. genitalium. 23 Numerous demographic and behavioral factors have been reported to be associated with M. genitalium infection. 8,13 In women, young age, with a low education level, originating from sub-Saharan Africa, having ≥6 sexual partners in the last 6 months, onset of sexual activity after 20 years of age, HIV positivity, and co-infection with other STIs have been associated with a higher risk of having M. genitalium infection.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Low prevalence of macrolide resistance in Mycoplasma genitalium infections in a cohort of pregnant women living with human immunodeficiency virus

et al. 2022

View full text Add to dashboard Cite

Objective Macrolide resistance in Mycoplasma genitalium ( M. genitalium) is increasing as a result of the widespread use of azithromycin in the treatment of sexually transmitted infections (STIs). To date, there are few published studies on macrolide resistance patterns in South African pregnant women. This study now contributes to the growing body of knowledge. Methods This study included 385 pregnant women living with HIV. Vaginal swabs were collected from consenting pregnant women and used for the detection of M. genitalium using the TaqMan assay . Macrolide resistance-associated mutations in the 23S rRNA gene were determined for all samples that tested positive for M. genitalium using the AllplexTM MG & AziR assay (Seegene) which allows for the simultaneous detection and identification of M. genitalium and six mutations ( A2058C, A2058G, A2058T, A2059C, A2059G and A2059T) responsible for azithromycin resistance. The correlation between the TaqMan assay and AllplexTM MG & AziR assay (Seegene) for the detection of M. genitalium was also performed in a subset of 121 samples. Results Of the 385 samples tested in this study, 14 samples were positive for M. genitalium estimating a prevalence of 3.6%. The same 14 samples also tested positive on the AllplexTM assay indicating a good correlation between the TaqMan Assay and the AllplexTM. Of the 14 positive samples, one sample carried a mutation at position A2059G denoting macrolide resistance in this pathogen. Mutations in the other regions of the 23S rRNA were not detected. All assay controls used in the mutation scanning produced the desired results showing the validity of the assay. Conclusion In this study, macrolide resistance in M. genitalium was detected. Despite the low prevalence of resistance determinants ongoing antimicrobial resistance surveillance is vital considering that azithromycin is used in the syndromic management for the treatment of vaginal discharge syndrome.

show abstract

“…22 A recent study conducted among pregnant women in KwaZulu-Natal, South Africa reported a prevalence of 5.9% for M. genitalium . 23…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Low prevalence of macrolide resistance in Mycoplasma genitalium infections in a cohort of pregnant women living with human immunodeficiency virus

et al. 2022

View full text Add to dashboard Cite

show abstract

Mycoplasma genitalium and Antimicrobial Resistance Among a Cohort of West African Men Who Have Sex With Men Using Preexposure Prophylaxis (CohMSM-PrEP ANRS 12369-Expertise France Study)

Baetselier

Vuylsteke

Cuylaerts

et al. 2022

Open Forum Infectious Diseases

View full text Add to dashboard Cite

Background Antimicrobial resistance to macrolides and fluoroquinolones in Mycoplasma genitalium among men who have sex with men (MSM) is worryingly high in high-resource countries. Data in Africa are lacking. We aimed to assess the burden of M. genitalium including the presence of resistance associated mutations (RAMs) in M. genitalium among MSM using HIV pre-exposure prophylaxis in Burkina Faso, Côte d’Ivoire, Mali and Togo. Methods MSM were included in a prospective cohort study (2017-2021). Molecular detection of M. genitalium in urine, anorectal, and pharyngeal samples was performed at baseline and after 6 and 12 months. Detection of RAMs to macrolides and fluoroquinolones was performed by sequencing the 23S rRNA, the parC and gyrA gene. A sample was found to be possibly resistant to fluoroquinolones if alterations were found in ParC position 83/87. Results Of 598 participants, 173 (28.9%) were positive at least once for M. genitalium and global point-prevalence was 19.4%. Interestingly, 238/250 (95.2%) infections were asymptomatic and 72/138 M. genitalium infections with follow-up data (52.2%) cleared during the study. Only one macrolide RAM was found (0.6%). Prevalence of fluoroquinolones RAMs was 11.3% overall, ranging from 2.4% in Burkina Faso to 17.5% in Mali. Conclusions Although M. genitalium was highly prevalent in these MSM, macrolide resistance was almost non-existent. Nevertheless, more than 10% of the samples was possibly resistant to fluoroquinolones. Heterogeneity in the prevalence of fluoroquinolone RAMs between countries may be explained by different antimicrobial consumption in humans and animals.

show abstract

Macrolide and fluoroquinolone resistance in Mycoplasma genitalium isolated from pregnant women in Russia and Belarus

Avchinnikova,

Edelstein,

Pokusaeva

et al. 2024

CMAC

View full text Add to dashboard Cite

Objective. To study epidemiology of resistance to macrolides and fluoroquinolones in Mycoplasmagenitalium isolated from pregnant women in Russia and Belarus. Materials and Methods. A total of 107 M. genitalium DNA samples isolated from pregnant women in Russia (n = 58) and Belarus (n = 49) was included in the study. In order to detect markers of macrolide or fluoroquinolone resistance, polymerase chain reaction in real time with the effect of quenching fluorescence of the probe by a primer was used. Confirmation of mutation type was performed using Sanger sequencing. Results. The prevalence of M. genitalium resistance mutations to macrolides in Russia (Tula, Smolensk) and Belarus (Vitebsk, Brest, Minsk) was 3.4% (2⁄58) and 6.1% (3⁄49), respectively. Markers of M. genitalium resistance to fluoroquinolones were detected in 5.2% (3⁄58) of samples from Russia and 8.2% (4⁄49) of samples from Belarus. The most common mutations associated with macrolide resistance were A2059G (n = 4), fluoroquinolone resistance – D84N (n = 3) and S80I (n = 3) (numbering according to E. coli). Conclusions. The prevalence of M. genitalium resistance markers isolated in pregnant women highlights a need for regular macrolide and fluoroquinolone resistance surveillance.

show abstract

Lack of resistance to macrolides in Mycoplasma genitalium detected in South African pregnant women

Cited by 3 publications

References 31 publications

Low prevalence of macrolide resistance in Mycoplasma genitalium infections in a cohort of pregnant women living with human immunodeficiency virus

Low prevalence of macrolide resistance in Mycoplasma genitalium infections in a cohort of pregnant women living with human immunodeficiency virus

Mycoplasma genitalium and Antimicrobial Resistance Among a Cohort of West African Men Who Have Sex With Men Using Preexposure Prophylaxis (CohMSM-PrEP ANRS 12369-Expertise France Study)

Macrolide and fluoroquinolone resistance in Mycoplasma genitalium isolated from pregnant women in Russia and Belarus

Contact Info

Product

Resources

About