Lack of <scp>ACTPK</scp>1, an <scp>STY</scp> kinase, enhances ammonium uptake and use, and promotes growth of rice seedlings under sufficient external ammonium

Beier, Marcel Pascal; Obara, Mitsuhiro; Taniai, Akiko; Sawa, Yuki; Ishizawa, Jin; Yoshida, H.; Tomita, Narumi; Yamanaka, T.; Ishizuka, Yawara; Kudo, Syuko; Yoshinari, Akira; Takeuchi, S.; Kojima, Shinichi; Yamaya, Tomoyuki; Hayakawa, Toshihiko

doi:10.1111/tpj.13824

Cited by 34 publications

(31 citation statements)

References 59 publications

(112 reference statements)

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“…10B . ACTPK1 (Os02g0120100) has been demonstrated to phosphorylate and inactivate the ammonium transporter AMT1;2 in rice seedling roots under sufficient ammonium conditions 31 . The Os02g0807000 gene encodes phosphoenolpyruvate carboxylase kinase 1 (PPCK1) that plays an important role in the regulation of phosphoenolpyruvate carboxylase (PEPC) and plant metabolism.…”

Section: Resultsmentioning

confidence: 99%

“…The Arabidopsis NIGT1 homolog is involved in the integration of nitrate and phosphate signals at the root tip 30 . More recently, the Os02g0120100 gene encoding ACT domain-containing protein kinase 1 (ACTPK1), a homolog of Arabidopsis serine/threonine/tyrosine kinase 46 (STY46), was identified by transcriptome analysis of ammonium-responsive genes in rice roots 31 . Further genetic and biochemical studies demonstrated that ACTPK1 can phosphorylate and inactivate AMT1;2, a major ammonium transporter, under ammonium-sufficient conditions 31 .…”

Section: Introductionmentioning

confidence: 99%

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Early molecular events associated with nitrogen deficiency in rice seedling roots

Hsieh

Kan

et al. 2018

Sci Rep

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Nitrogen (N) deficiency is one of the most common problems in rice. The symptoms of N deficiency are well documented, but the underlying molecular mechanisms are largely unknown in rice. Here, we studied the early molecular events associated with N starvation (−N, 1 h), focusing on amino acid analysis and identification of −N-regulated genes in rice roots. Interestingly, levels of glutamine rapidly decreased within 15 min of −N treatment, indicating that part of the N-deficient signals could be mediated by glutamine. Transcriptome analysis revealed that genes involved in metabolism, plant hormone signal transduction (e.g. abscisic acid, auxin, and jasmonate), transporter activity, and oxidative stress responses were rapidly regulated by −N. Some of the −N-regulated genes encode transcription factors, protein kinases and protein phosphatases, which may be involved in the regulation of early −N responses in rice roots. Previously, we used similar approaches to identify glutamine-, glutamate-, and ammonium nitrate-responsive genes. Comparisons of the genes induced by different forms of N with the −N-regulated genes identified here have provided a catalog of potential N regulatory genes for further dissection of the N signaling pathwys in rice.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Early molecular events associated with nitrogen deficiency in rice seedling roots

Hsieh

Kan

et al. 2018

Sci Rep

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“…Members of the STY kinase family are dual-specificity kinases that possess catalytic Ser/Thr and Tyr domains, and they regulate some plant metabolic and developmental processes through phosphorylation of target proteins [24,25]. For example, STY protein kinases appear to have important roles in ammonium transport in rice seedling roots [26], in storage oil accumulation in Arabidopsis siliques [27], and in stress response and seed development in cucumber and peanuts [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response

Dong

Zhang

Beckles

2020

Plants

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Starch provides plants with carbon and energy during stressful periods; however, relatively few regulators of starch metabolism under stress-induced carbon starvation have been discovered. We studied a protein kinase Ser/Thr/Tyr (STY) 46, identified by gene co-expression network analysis as a potential regulator of the starch starvation response in Arabidopsis thaliana. We showed that STY46 was induced by (1) abscisic acid and prolonged darkness, (2) by abiotic stressors, including salinity and osmotic stress, and (3) by conditions associated with carbon starvation. Characterization of STY46 T-DNA knockout mutants indicated that there was functional redundancy among the STY gene family, as these genotypes did not show strong phenotypes. However, Arabidopsis with high levels of STY46 transcripts (OE-25) grew faster at the early seedling stage, had higher photosynthetic rates, and more carbon was stored as protein in the seeds under control conditions. Further, OE-25 source leaf accumulated more sugars under 100 mM NaCl stress, and salinity also accelerated root growth, which is consistent with an adaptive response. Salt-stressed OE-25 partitioned 14C towards sugars and amino acids, and away from starch and protein in source leaves. Together, these findings suggested that STY46 may be part of the salinity stress response pathway that utilizes starch during early plant growth.

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“…Hsieh et al [60] found that the N-regulated genes, Os02g0120100, Os02g0807000 and Os06g0692600 etc., which encode transcription factors, protein kinases and protein phosphatases and may be involved in the regulation of early −N responses in rice roots. The serine/threonine/tyrosine (STY) protein kinase, ACTPK1, enhances ammonium uptake and use, and promotes growth of rice seedlings under sufficient ammonium [61] . A calcium-dependent protein kinase gene, esl4, which may function upstream of nitrogen-metabolism genes [62] .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of the peptide transporter family in rice and analysis of the PTR expression modulation in two near-isogenic lines with different nitrogen use efficiency

Xiao-ling

Xia

Zeng

et al. 2020

Preprint

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Background: Nitrogen (N) is a major nutrient element for crop growth. In plants, the members of the peptide transporter (PTR) gene family are involved in nitrate uptake and transport. Here, we identied PTR gene family in rice and analyzed their expression level in near-isogenic lines. Results: We identified 96, 85 and 78 PTR genes in Nipponbare, R498and Oryza glaberrima , and the phylogenetic were similar in Asian cultivated rice and African cultivated rice. The number of PTR genes was higher in peanuts (125) and soybeans (127). The 521 PTR genes in rice, maize, sorghum, peanut, soybean and Arabidopsis could be classified into 4 groups, and their distribution was different between monocots and dicots. In Nipponbare genome, the 25 PTR genes were distributed in 5 segmental duplication regions on chromosome 1, 2, 3, 4, 5, 7, 8, 9, and 10. The PTR genes in rice have 0-11 introns and 1-12 exons , and 16 of them have the NPF (NRT1/PTR family) domain. The results of RNA-Seq showed that the number of differentially expressed genes (DEGs) between NIL15 and NIL19 at three stages were 928, 1467, and 1586, respectively. Under low N conditions, the number of differentially expressed PTR genes increased significantly. The RNA-Seq data was analyzed using WGCNA to predict the potential interaction between genes. We classified the genes with similar expression pattern into one module, and obtained 25 target modules. Among these modules, three modules may be involved in rice nitrogen uptake and utilization, especially the brown module, in which hub genes were annotated as protein kinase that may regulate rice N metabolism. Conclusions: In this study, we comprehensively analyzed the PTR gene family in rice. 96 PTR genes were identified in Nippobare genome and 25 of them were located on five large segmental duplication regions. The Ka/Ks ratio indicated that many PTR genes had undergone positive selection. The RNA-seq results showed that many PTR genes were involved in riceNUE, and protein kinases might play an important role in this process. These results provide a fundamental basis to improve the rice NUE via molecular breeding.

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Lack of ACTPK1, an STY kinase, enhances ammonium uptake and use, and promotes growth of rice seedlings under sufficient external ammonium

Cited by 34 publications

References 59 publications

Early molecular events associated with nitrogen deficiency in rice seedling roots

Early molecular events associated with nitrogen deficiency in rice seedling roots

A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response

Genome-wide identification of the peptide transporter family in rice and analysis of the PTR expression modulation in two near-isogenic lines with different nitrogen use efficiency

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