2013
DOI: 10.1099/vir.0.057182-0
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Lack of the host membrane protease FtsH hinders release of the Lactococcus lactis bacteriophage TP712

Abstract: The temperate bacteriophage TP712 was unable to plaque on Lactococcus lactis DftsH lacking the membrane protease FtsH and complementation in trans restored the WT phenotype. Absence of ftsH did not hinder phage adsorption, phage DNA delivery or activation of the lytic cycle. Thin sections revealed that TP712 virions appeared to be correctly assembled inside the DftsH host, but were not released. These virions were infective, demonstrating that a functional host FtsH is required by TP712 to proceed effectively … Show more

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Cited by 12 publications
(23 citation statements)
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“…3). Interestingly, they are all related to the temperate phage ɸTP712 found in the widely used plasmid-free laboratory strain L. lactis MG1363 and derived from the dairy L. lactis strain NCDO 712 32, 33 . Phage ɸTP712 is also related to the sequenced temperate genome, PLgT-1, isolated from a marine environment (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…3). Interestingly, they are all related to the temperate phage ɸTP712 found in the widely used plasmid-free laboratory strain L. lactis MG1363 and derived from the dairy L. lactis strain NCDO 712 32, 33 . Phage ɸTP712 is also related to the sequenced temperate genome, PLgT-1, isolated from a marine environment (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…AbiF is a protein involved in phage abortive infection [see review by Labrie et al (2010)]. Homologs of this gene are not present in any of the other C. difficile phages and while Abi genes are typically encoded by their bacterial hosts, they have been found on a prophage element in Lactococcus lactis (Ventura et al, 2007) and in the genome of L. lactis phage TP712 (Roces et al, 2013). Although the mechanism by which AbiF performs its function is not fully known (Garvey et al, 1995), the unusual presence of this gene in the genome of phage φC2 suggests it could inhibit secondary phage infection or modulate φC2 replication and may contribute to the low frequencies reported for isolating free phages.…”
Section: Genomic Features Of the C Difficile Phagesmentioning
confidence: 99%
“…Causing ablation of this promoter was aimed at preventing t712 prophage induction and virion production. Induction of prophage t712 was identified as a potential problem as; (i) t712 particle production would interfere with TP901-1 phage particle study, (ii) t712 induction would increase the metabolic burden on the host when only TP901-1 induction was desired, and (iii) it was deemed possible that, due to the conserved nature of Siphoviridae proteins, t712 may supply TP901-1 with structural proteins being analysed during this study in trans [ 59 ]. This expectation was indeed verified, as mutagenesis of the deduced promoter sequence for t712 Cro protein caused an approximate 100-fold increase in the number of produced infective TP901-1 erm phage particles obtained following mitomycin C induction from the NZ9000-Cro t712 background as compared to that obtained when strain NZ9000 was used (data not shown; indicator strain used for TP901-1 erm phage titre determination was L .…”
Section: Resultsmentioning
confidence: 99%
“…Inducible prophage t712 of NZ9000 [ 58 ], called TP712 by Roces et al . (2013) [ 59 ], was identified using the PHAge Search Tool (PHAST) [ 60 ]. BLAST, Pfam and HHpred analyses were used for functional annotations of proteins [ 61 64 ].…”
Section: Methodsmentioning
confidence: 99%