Introduction: In this research, the issue of the role of nursing in breastfeeding in hospitalized neonates is addressed. Breastfeeding plays a fundamental role in the health and development of hospitalized neonates, and adequate support from nursing staff is essential to promote and facilitate this practice. Objective: To explore the experiences and perceptions of mothers, fathers and nursing staff in relation to breastfeeding in hospitalized neonates, as well as to evaluate the correlation between a good latch of the neonate and the production of breast milk. Methodology: Through a mixed methodological approach that combines qualitative and quantitative methods, to obtain a complete and holistic understanding of this important issue to improve neonatal care. Through in-depth interviews, focus groups, and structured questionnaires, the experiences and perceptions of mothers, fathers, and nursing staff were explored, as well as the correlation between newborn latch-on and breast milk production. Data analysis revealed that the instruments used in this study showed good reliability, with Cronbach's coefficients higher than 0.8. Results: They showed common challenges in breastfeeding, such as the lack of information and emotional support. A significant correlation was found between a good latch of the newborn and a higher production of breast milk. Conclusion: The training of nursing staff and the implementation of support strategies, such as the use of lactation consultants, were identified as key elements to improve breastfeeding care in the hospital setting. These findings support the importance of the role of nursing in the promotion and support of breastfeeding in hospitalized neonates.