Lactate and Pyruvate Metabolism in Dog Renal Outer Medulla. Effects of Oleate and Ketone Bodies

Baverel, Gabriel; Forissier, M; Pellet, M

doi:10.1016/b978-0-08-025517-0.50037-0

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…The glomeruli were retained and further purified by successive washings. Thick ascending limbs were obtained directly from digestion of the red medulla (33). Distal collecting ducts were derived from the white medulla.…”

Section: Introductionmentioning

confidence: 99%

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Willenbrock

Tremblay²,

García³

et al. 1989

J. Clin. Invest.

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The hypotensive, natriuretic, and diuretic actions of human atrial natriuretic factor499-126) (hANF) Met-O-ANF doses, which led to a significant increase in diuresis, were neither natriuretic nor accompanied by an increase of urinary cGMP. We were thus able to dissociate the diuretic and natriuretic effects of ANF. High doses of the oxidized analogue were required to elevate cGMP levels in plasma and urine. In isolated kidney fractions, Met-O-ANF's action on cGMP was significantly lower in glomeruli (fivefold less), virtually absent in the collecting duct, yet only slightly different (20% less) in thick ascending limb. Our results indicate that the diuretic and natriuretic effects are exerted at distinct sites, with only the natriuresis being related to an increase of extracellular cGMP. The variability of differential potency of biological and biochemical effects from tissue to tissue of these two forms of human ANF support the notion of the heterogeneity of the ANF effector system.

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Section: Introductionmentioning

confidence: 99%

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Willenbrock

Tremblay²,

García³

et al. 1989

J. Clin. Invest.

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“…In vivo, however, the dog kidney [6] ox idizes glucose at the rate of ~0.06pm ol/ (g • min). This lower rate may be due to a spe cies difference, or to the influence of the avail ability of alternative (preferred) fuels [2,3,25]. Indeed, with the exception of the group with the lowest [glucose]p, the Qox values for the early observation period (20-50min) were lower than those of the subsequent observa tion interval (50-160min) ( fig.…”

Section: Glucose Decarboxylationmentioning

confidence: 87%

Kinetics of Glucose Decarboxylation in the Substrate-Limited Isolated Perfused Kidney

Anaizi¹,

Cohen²

1991

Kidney Blood Press Res

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Rat kidneys were perfused with a cell-free perfusate containing substrate-free albumin, different glucose concentrations (0.20–5.0 mmol/l), and uniformly labeled ¹⁴C-glucose. The rate of glucose decarboxylation (Q_ox), as a function of [glucose]_p, displayed saturation kinetics [V_max = 0.35 µmol/(g · min); K_m = 0.87mmol/l]; saturation occurred at [glucose]_p = 1.0–2.0mmol/l. Although the presence of as low as 0.2mmol/l of glucose significantly increased fractional sodium reabsorption (%T_Na), there was no correlation between [glucose]_p or Q_ox and %T_Na. However, free water clearance (C_H2O or C_H2O/GFR) was directly proportional to [glucose]_pand independent of Q_ox. We conclude that (1) in the absence of other substrates, renal glucose Q_oxsaturates at hypoglycemic levels of glucose and (2) glucose plays an important role in the generation of solute-free water, a role that is unrelated to glucose Q_ox.

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“…However, this number reflects the weight of extracellular osmolcs that are not present in the suspension in vitro. This tubule preparation was previously used by us |5] and similar preparations were used by others [6].…”

Section: Preparation O F Suspension O F Tal From the Dog Kidneymentioning

confidence: 99%

“…Ohio), filled with freshly gassed KHS. The fall in PO: was recorded on paper or directly memorized and analyzed by a desk computer (Apple II) as described [6]. The tubules were left in the ox ymeter for 5-30 min.…”

Section: Measurement O F Respirationmentioning

confidence: 99%

Metabolism of Lactate by a Suspension of Dog Thick Ascending Limbs: Relations with Transport

Tejedor

Noël²,

Sénécal³

et al. 1991

Kidney Blood Press Res

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The addition of substrate in the form of lactate (L), but not glucose (G), increases the respiration of canine thick ascending limb (TAL) segments in a saturable (above 2 mM) fashion. More than 60% of this stimulation is ouabain-sensitive (1 mM ouabain) even if L and G transport are both sodium-insensitive processes in TAL. Thus L, but not G, specifically stimulates Na⁺ entry in TAL cells and its subsequent transport by the Na⁺, K⁺-ATPase. If chloride is substituted for by gluconate, no significant substrate-induced stimulation of ouabain-sensitive respiration is observed. SITS (4-acetamino-4’-isothiocyanostilbene-2,2’-disulfonic acid) also interferes with the L-induced stimulation of respiration. Thus L entry in TAL appears to be directly or indirectly coupled to the transepithelial flux of Cl^-. Furosemide (F) but not amiloride, also inhibits this stimulation suggesting that the accelerated Na⁺ entry triggered by the application of L occurs through the F-sensitive carrier or that lactate transport is F-sensitive in TAL cells. In accord, F specifically impairs the metabolism of L (as compared to G). These data suggest that in intact TAL tubules both lactate uptake and oxidation are directly or indirectly influenced by the transcellular flux of NaCl. This organization may participate to maintain a stoichiometry between the transport of NaCl and the availability of L to support the energetic needs of TAL cells.

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Lactate and Pyruvate Metabolism in Dog Renal Outer Medulla. Effects of Oleate and Ketone Bodies

Cited by 4 publications

References 29 publications

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Dissociation of natriuresis and diuresis and heterogeneity of the effector system of atrial natriuretic factor in rats.

Kinetics of Glucose Decarboxylation in the Substrate-Limited Isolated Perfused Kidney

Metabolism of Lactate by a Suspension of Dog Thick Ascending Limbs: Relations with Transport

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