Lactate dehydrogenase-catalyzed regeneration of NAD from NADH for use in enzyme-catalyzed synthesis

Study of NADH Stability Using Ultraviolet–Visible Spectrophotometric Analysis and Factorial Design

“…Another important aspect involving NADH stability under different conditions of preparation is the convenience of the coenzyme regeneration associated with organic synthesis and enzymatic reactions (40,41). In many cases, when the NADH is employed as coenzyme in dehydrogenase-catalyzed reactions (42), it is possible to perform the molecule regeneration by utilization of coupled enzyme systems, turning these methods to be more economical for use in enzymatic analysis.…”

Mathematical modelling of the dehydrogenase catalyzed hexanol oxidation with coenzyme regeneration by NADH oxidase

“…(4) and Eqs. (10)- (12). The model includes a balance equation for the hexanoic acid production (Eq.…”

“…On the other hand, the various enzymes such as alcohol dehydrogenase, lactate dehydrogenase and glutamate dehydrogenase [3,[11][12][13][14][15] are used for the regeneration of NAD + . NADH oxidases [16][17][18][19] catalyze the reaction of NADH oxidation with oxygen, where the products are NAD + , water or hydrogen peroxide (depending on the enzyme source).…”

Engineering an enzymatic regeneration system for NAD(P)H oxidation

“…As suggested by Chenault and Whitesides [8] an ideal enzymatic regeneration system should meet the following criteria: (i) the enzymes should be inexpensive and stable, (ii) the enzymes should have high specific activity, (iii) simple and inexpensive reagents that do not interfere with the isolation of the product of interest or with enzyme stability should be employed, (iv) high turnover numbers should be obtained, (v) the total turnover number of the coenzyme should be at least between 10 2 and 10 4 , and (vi) an overall equilibrium for the coupled enzyme system favorable to product formation should be reached. These criteria have been already partially met for NAD + -reducing enzymes such as alcohol dehydrogenase, lactate dehydrogenase and glutamate dehydrogenase [7,9,10]. However, the enzymatic oxidation of NAD(P)H is not satisfactorily developed to date.…”