Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate

Faddah, L. M.; Abdel-Hamid, N.; Abul-Naga, Yaser; Ibrahim, Sally; Mahmoud, Ahmed

doi:10.32725/jab.2007.010

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…These results are in accordance with previous studies (Karagoz et al, 2015,Wahid et al, 2016 and the increased levels of these en-zymes in serum of the CCl4 treated rats indicated liver damage which is always associated with hepatonecrosis (Naik and Panda, 2008). However, LDH enzyme is an oxidoreductase enzyme that catalyzes the conversion of pyruvate and lactate in the liver in addition to a number of other tissues in which its level was a b c d e f increased following liver damage (Faddah et al, 2007). When the different cytosolic liver enzymes were leaked out from the swollen and necrotic hepatocytes into the blood, their levels were elevated and related to the centrilobular necrosis, degradation and cellular infiltration of the liver (Khan et al, 2013).…”

Section: Discussionsupporting

confidence: 92%

Effect of aqueous extract of olive leaves on some biochemical changes induced by carbon tetrachloride in male rats

Ashour

Mohamed

2019

J. Exper. Appl. Anim. Sci.

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Olive tree leaves have been widely used in traditional remedies in European and Mediterranean countries and in the human diet as extracts, herbal teas, and powder, which contain several potential bioactive compounds that may have antihypertensive, antiatherogenic, anti-inflammatory, hypoglycemic, hypocholesterolemic, antidiabetic and antioxidant activities. The present study was carried out to evaluate the role of olive leaves extracts (OLE) in ameliorating the metabolic changes in rats treated with carbon tetrachloride (CCl4). Forty-eight male rats were classified into six groups; the first group was the control which received 1 ml distilled water, the second group was treated daily with oral dose of cold OLE (1 ml/kg) for one month, the third group was treated daily with oral dose of boiled OLE (1 ml/kg) for one month, the fourth group was treated daily with oral dose of CCl4 (1 ml/kg) for one month, the fifth group was treated with both cold OLE and CCl4 (1 ml/kg for each) for one month and the sixth group was treated with both boiled OLE and CCl4 (1 ml/kg for each) for one month. Serum levels of thiobarbituric acid reactive substance (TBARS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), urea, creatinine, insulin and glutathione (GSH), catalase (CAT), albumin, total protein, free triiodothyronine (FT3) , free thyroxin (FT4) were measured in serum. Histological examination of hepatic tissue was examined. The results showed that administration of CCl4 to rats induced significant increase (P<0.05) in serum levels of thiobarbituric acid reactive substance (TBARS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma- glutamyl transferase (GGT), lactate dehydrogenase (LDH), urea, creatinine, insulin and significant decrease (P<0.05) in glutathione (GSH), catalase (CAT), albumin, total protein, free triiodothyronine (FT3) and free thyroxin (FT4). The histological examination of hepatic tissue showed some degenerative changes while significant improvement in biochemical and histological changes was observed either in CCl4 + cold OLE or CCl4 + boiled OLE groups. It could be concluded that the protective effect of olive leaves extract either cold or boiling may be attributed to its antioxidant properties. The obtained results indicated that both cold and boiled olive leaves extract has potent effect to restore the antioxidant status, hepato-renal function, insulin and thyroid hormones near to the control levels.

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Section: Discussionsupporting

confidence: 92%

Effect of aqueous extract of olive leaves on some biochemical changes induced by carbon tetrachloride in male rats

Ashour

Mohamed

2019

J. Exper. Appl. Anim. Sci.

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“…Analysis also revealed LDH to be a suitable biomarker for cell damage. Faddah et al (2007) using carbon tetrachloride (CCl 4 ), an active ingredient of chlorinated pesticides, revealed a significant increase in the activity of liver total LDH activity in CCl 4 -intoxicated rats. The same effect was observed in rats using a chlorocamphene; a chloroorganic pesticide (Kuz'minskaya and Alekhina, 1976).…”

Section: Discussionmentioning

confidence: 99%

Impact of dieldrin on liver morphological and biochemical parameters of rats

et al. 2010

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The current study deals with the effect of the organochlorine insecticide on the liver of Wistar rats. The dieldrin effect on rats was tested after a single intraperitoneal (i.p.) injection of two doses: 3 and 6 mg/kg and observations were made 4 days later. Animals showed a significant dose-dependent increase in relative liver weight. Elevations of transaminases (aspartate aminotransferase [AST], alanine aminotransferase [ALT]), bilirubin and total activity of lactate dehydrogenase (LDH) were recorded in the sera of treated rats. Serum LDH-5 isoenzyme activity increases in a dose-dependent manner. In contrast, LDH-1 activity does not show any significant variations with respect to controls. Histological examination of the liver of dieldrin-treated animals revealed cytoplasmic vacuolation, focal necrosis and nuclear enlargement of hepatocytes. This study suggests that biochemical assessment (transaminases, LDH and bilirubin activity) and LDH (LDH-1 & LDH-5) isoenzyme profiles can be very helpful in defining the border of the liver injury, dieldrin damaged liver would be a valuable addition to histological analysis in evaluating histopathological liver changes.

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“…A slight modification of the reported method (Faddah et al ., ) was used to homogenize the tissue samples. Briefly, tissues (50 mg ml −1 ) were homogenized by a homogenizer in a buffer containing 5 m m Tris–Cl (pH 7.4) and 150 m m NaCl.…”

Section: Methodsmentioning

confidence: 99%

Acute toxicity and tissue distribution of CdSe/CdS‐MPA quantum dots after repeated intraperitoneal injection to mice

Haque

Seo

et al. 2012

J of Applied Toxicology

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Quantum dots (QDs) are novel tools with multiple biological and medical applications because of their superior photoemission and photostability characteristics. However, leaching of toxic metals from QDs is of great concern. Therefore, for the successful application of QDs in bioscience, it is essential to understand their biological fate and toxicity. We investigated toxicological effects and tissue distribution of mercaptopropionic acid‐conjugated cadmium selenide/cadmium sulfide (CdSe/CdS‐MPA) QDs after repeated intraperitoneal injection into BALB/c mice. The mice were injected every 3 days with various doses of QDs (0, 5, 10 and 25 mg kg−1). The subsequent effects of QDs on plasma levels of various biomarkers were evaluated at different time points (at 0, 1, 4, 7, 10, 13 and 15 days). Various tissue samples (spleen, liver, lung, kidneys, brain, heart and thymus) were collected for toxicity analysis, distribution testing, histopathological examination and inflammation assessment. No abnormal clinical signs or behaviors were recorded but the body weight of mice treated with 25 mg kg−1 QDs was significantly decreased from day 7 compared with control mice. QDs were observed in the liver, spleen, lung and kidneys, but not in brain or heart. Significantly higher levels of lactate dehydrogenase and nicotinamide adenine dinucleotide phosphate oxidase were found in the plasma, liver and spleen. Histopathological examination did not show any tissue toxicity but the levels of interleukin‐6, a pro‐inflammatory marker, were increased in the plasma, liver and spleen. All of these findings provide insight into the observed toxicological effect levels and tissue‐specific distribution of CdSe/CdS‐MPA QDs. Copyright © 2012 John Wiley & Sons, Ltd.

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Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate

Cited by 9 publications

References 13 publications

Effect of aqueous extract of olive leaves on some biochemical changes induced by carbon tetrachloride in male rats

Effect of aqueous extract of olive leaves on some biochemical changes induced by carbon tetrachloride in male rats

Impact of dieldrin on liver morphological and biochemical parameters of rats

Acute toxicity and tissue distribution of CdSe/CdS‐MPA quantum dots after repeated intraperitoneal injection to mice

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