Lactate Dehydrogenase Output of the Excised Kidney as an Index of Acute Ischaemic Renal Damage

Kohn, Mary; Ross, Harry

doi:10.1097/00007890-197105000-00005

Cited by 24 publications

(4 citation statements)

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“…Since Belzer's study in 1968 the determina tion o f LDH in perfusate has been well known (4). Its applicability for experi mental and clinical purposes was tested by several authors (21,22). In our earlier investigations in dogs we were able to observe that the increase o f LDH during the pulsatile perfusion is a good indicator for viability of the organ (12,13).…”

Section: Discussionmentioning

confidence: 99%

Clinical Experiences in the Gambro-Preservation Unit: Analysis of 101 Human Cadaver Kidneys

Horpácsy¹,

Scholz²,

Althaus³

et al. 1979

Eur Surg Res

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101 human cadaver kidneys were preserved by means of hypofhermic pulsatile perfusion in the Gambro perfusion unit with human albumin as perfusate. Perfusate flow and some biochemical parameters were assessed during perfusion and correlated with renal function after transplantation. There is a good correlation between perfusate flow and recovery of physiological renal function. LDH released to the perfusate as viability test is of limited value: a direct correlation was only found between LDH activity and perfusion time, and LDH activity and ischemia time. Determination of pH and lactic acid level 1 h after beginning of perfusion is without practical value according to our experience. The quality of albumin in perfusion solution influences the immediate graft function.

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Section: Discussionmentioning

confidence: 99%

Clinical Experiences in the Gambro-Preservation Unit: Analysis of 101 Human Cadaver Kidneys

Horpácsy¹,

Scholz²,

Althaus³

et al. 1979

Eur Surg Res

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“…There is a vast increase in levels of acid phosphatase and fl-glueuronidase in the blood of the renal vein, a result that is not found in any other group studied. The liberation of lysosomal enzymes under the influence of isehaemia has previously been demonstrated in the intestine [3,35], but does not appear to have been studied in the renal vein blood after renal ischaemia: Other workers have studied various non-lysosomal enzymes, and have concluded that their increase in the renal vein was due to cellular destruction [14,19,20].…”

Section: Renal Ischaemiamentioning

confidence: 99%

The response of the dog kidney to transplant rejection or ischaemia: An experimental and comparative study with functional, microcirculatory and enzymological investigations

1972

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Abstract. 1. The effects of transplant rejection or of total ischaemia on the dog kidney were compared by studying the following parameters: urinary clearances, microcirculation, histological aspect, blood levels of lysosoma] enzymes, active transport of cortical slices, and levels of cortical ATPases.2. At the point of rejection, allotransplanted kidneys without immunosuppressire therapy maintained almost normal active transport of glycine and fl-methylglucoside by the proximal tubular epithelium, despite greatly reduced microcirculation and abolition of glomerular filtration.3. 1 or 2 hours' ischaemia provoked partial tubular necrosis with an abrupt liberation of lysosomal enzymes into the circulation and a decrease in active transport. The mierocirculation was less damaged. 2 days later, in spite of the partial recovery of the active transport, and discrete zones of cellular regeneration, the clearances were still non-existent.4. The results suggest that there is little correlation between the effects of allotransp]ant rejection and ischaemia. The primary event in the process of rejection involves the interruption of the glomerular circulation, which leads to a rapid cessation of urine formation, without too much damage to the transport function of the tubular epithelium.

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“…Furthermore, MP offers the opportunity of viability assessment. Intrarenal vascular resistance (IRR) [I21 and release of the enzymes lactate dehydrogenase (LDH) [15] and a-glutathione S-transferase (aGST) [9,141 into the perfusate can be analysed for viability testing. Besides these tests, donor data and macroscopic and microscopic appearance of the kidney remain important criteria for accepting or discarding an organ for transplantation.…”

Section: Introductionmentioning

confidence: 99%

The value of dextran 12,000 in ischemically damaged canine kidneys during machine perfusion

Hermens¹,

Kievit²,

Nederstigt³

et al. 1999

Transplant International

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The use of non-heartbeating (NHB) donor kidneys has led to the search for new methods of viability-testing. We investigated, in a canine model, the relationship between the filtration of dextran 12, 000 into urine and a certain period of warm ischemic time (WIT) during machine perfusion. Twenty-four canine kidneys were divided into three groups, sustaining 0 min, 30 min or 60 min of WIT. After cooling and flushing, the kidneys were perfused on a perfusion machine for 8 h. Three hundred milligrams of dextran 12,000 was added to the perfusate. In the perfusate, dextran and lactate dehydrogenase (LDH) concentrations were measured. Dextran concentrations were also analysed in urine. Intrarenal vascular resistance (IRR) was calculated from pressure and flow characteristics. The 30WIT group showed a higher dextran excretion rate than the other two groups. IRR and LDH measurements showed lower levels in the ischemic groups compared with the control group. Dextran 12,000 is not suitable as a viability test but does show interesting results regarding the low LDH and IRR levels in the ischemic groups.

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Lactate Dehydrogenase Output of the Excised Kidney as an Index of Acute Ischaemic Renal Damage

Cited by 24 publications

References 0 publications

Clinical Experiences in the Gambro-Preservation Unit: Analysis of 101 Human Cadaver Kidneys

Clinical Experiences in the Gambro-Preservation Unit: Analysis of 101 Human Cadaver Kidneys

The response of the dog kidney to transplant rejection or ischaemia: An experimental and comparative study with functional, microcirculatory and enzymological investigations

The value of dextran 12,000 in ischemically damaged canine kidneys during machine perfusion

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