Lactic acid bacteria in the inhibition of Fusarium graminearum and deoxynivalenol detoxification

Abstract

Aims: Considering the agronomic and industrial damage that is caused by the fungus Fusarium graminearum, as well as the serious health risks it poses to humans and animals exposed to F. graminearum‐produced mycotoxin deoxynivalenol (DON), this study evaluated the ability of different lactic acid bacteria (LAB) strains to inhibit fungal development and remove DON in vitro. Methods and Results: The antagonistic effects of strains and commercial cultures of LAB were evaluated against F. graminearum IAPAR 2218 by the agar diffusion method. Additionally, the influence of the culture media, pH and the presence of lactic and acetic acid on these effects was tested. The capacity to remove DON by viable cells and heat‐inactivated cells was analysed in liquid media and quantified by high performance liquid chromatography (HPLC). All isolated strains and commercial cultures inhibited the fungus and removed DON. The pH and culture media concentration did not influence these abilities, but heat inactivation had a strong effect on the ability of bacteria to remove mycotoxin. Conclusions: The isolated bacteria are able to inhibit F. graminearum growth and remove DON in vitro. Significance and Impact of the Study: This study suggests potential application of the isolated LAB strains in the inhibition of F. graminearum IAPAR 2218 and DON removal in vitro.

The Effect of Experimental Fusarium Mycotoxicosis on Microbiota Diversity in Porcine Ascending Colon Contents

“…LAB play a beneficial role for the host, producing substances such as short-chain fatty acids (acetic, butyric, propionic), amino acids, and B vitamins, as well as other antimicrobial metabolites, such as bacteriocins, which prevent colonization of the digestive tract by pathogens and stimulate the immune system of the host [21,39,40,41]. Furthermore, LAB can bind mycotoxins, including Fusarium toxins, from their environment [42,43]. The capacity to remove of above 60% of DON initial concentration (1.5 µg/mL) from model liquid media by viable and heat-inactivated cells of Lactobacillus species was reported [43].…”

“…Furthermore, LAB can bind mycotoxins, including Fusarium toxins, from their environment [42,43]. The capacity to remove of above 60% of DON initial concentration (1.5 µg/mL) from model liquid media by viable and heat-inactivated cells of Lactobacillus species was reported [43]. According to El-Nezami and co-workers [44] this phenomenon occurs as a result of mycotoxin adsorption to bacterial cell wall.…”

Transcriptomic profiling to identify genes involved in Fusarium mycotoxin Deoxynivalenol and Zearalenone tolerance in the mycoparasitic fungus Clonostachys rosea

“…Several bacteria have also been found to be capable of detoxifying DON. These included a Bacillus sp., Lactobacillus pentosus , L. paracasei and L. plantarum [18]. Thus, de-epoxidation, epimerization and mineralization are reviewed as three important DON detoxifying mechanisms in microorganisms [19-21].…”

Selection of lactic acid bacteria to promote an efficient silage fermentation capable of inhibiting the activity of Aspergillus parasiticus and Fusarium gramineraum and mycotoxin production

“…; Franco et al . ). In the LAB strains ‐ A. parasiticus interactions, LAB strains were less effective as antagonistic agents, reducing fungal growth rate only at certain interacting conditions, mainly at the higher water activity tested.…”

“…Fusarium graminearum is globally recognized as one of the most important fungal pathogens of grains and cereals, causing great agricultural losses (Franco et al . ). Zearalenone is a Fusarium‐ produced mycotoxin that has a chemical structure similar to oestrogen and can produce an oestrogenic response in animals.…”