Lactic Acid Bacteria (LAB) Bind to Human B- or H-Antigens Expressed on Intestinal Mucosa

Uchida, Hideaki; Kawai, Yasushi; Kinoshita, Hideki; Kitazawa, Haruki; Miura, Koh; Shiiba, Kenichi; Horii, Akira; Kimura, Katsunori; Taketomo, Naoki; Oda, Munehiro; Yajima, Takaji; Saito, Tadao

doi:10.1271/bbb.60407

Cited by 30 publications

(26 citation statements)

References 7 publications

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“…This is also supported by the fact that several lactobacilli specifically bound to different HBGAs [26]–[27]. We investigated how the presence of probiotic bacteria influences the binding of NoVs to target cells by using the P-particles and cultured intestinal epithelial cells as a model.…”

Section: Resultsmentioning

confidence: 99%

“…Enhancement of innate or adaptive immunity has emerged as a mechanism by which probiotics can counteract gastrointestinal viral infections [16], [21]–[25], but it is also postulated that they can interfere with viral infection by competing with binding to viral receptors on the surface of host cells or by direct binding to viral particles, promoting their clearance from the intestine. Certain Lactobacillus brevis , Lactobacillus gasseri , Lactobacillus salivarius and Lactobacillus acidophillus strains isolated from human feces are able to bind type-A, -B and -H HBGAs through the lectin-like activity of their surface layer proteins [26]–[27] for which they might compete with NoVs for the attachment sites at the intestinal mucosa. Recently, an Enterobacter sp.…”

Section: Introductionmentioning

confidence: 99%

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Noroviral P-Particles as an In Vitro Model to Assess the Interactions of Noroviruses with Probiotics

et al. 2014

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Noroviruses (NoVs) are the main etiologic agents of acute epidemic gastroenteritis and probiotic bacteria have been reported to exert a positive effect on viral diarrhea. The protruding (P) domain from NoVs VP1 capsid protein has the ability to assemble into the so-called P-particles, which retain the binding ability to host receptors. We purified the P-domains from NoVs genotypes GI.1 and GII.4 as 6X(His)-tagged proteins and determined that, similar to native domains, they were structured into P-particles that were functional in the recognition of the specific glycoconjugated receptors, as established by surface plasmon resonance experiments. We showed that several lactic acid bacteria (probiotic and non-probiotic) and a Gram-negative probiotic strain have the ability to bind P-particles on their surfaces irrespective of their probiotic status. The binding of P-particles (GI.1) to HT-29 cells in the presence of selected strains showed that bacteria can inhibit P-particle attachment in competitive exclusion experiments. However, pre-treatment of cells with bacteria or adding bacteria to cells with already attached P-particles enhanced the retention of the particles. Although direct viral binding and blocking of viral receptors have been postulated as mechanisms of protection against viral infection by probiotic bacteria, these results highlight the need for a careful evaluation of this hypothesis. The work presented here investigates for the first time the probiotic-NoVs-host interactions and points up the NoVs P-particles as useful tools to overcome the absence of in vitro cellular models to propagate these viruses.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Noroviral P-Particles as an In Vitro Model to Assess the Interactions of Noroviruses with Probiotics

et al. 2014

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“…Those genes included a hypothetical membrane protein with possible transport function (BAD 1206), a capsular polysaccharide biosynthesis protein Cps4H (BAD 1388), and a protein possibly involved in ATP-driven polysaccharide export (BAD 1500). The cell surface molecules can play a role in host-bacterium or interbacterial interaction, such as Lactobacilli binding to human blood type antigens expressed in the intestinal mucosa, 4) or the specific binding of L. brevis S-layer protein (SlpA) to human blood type A-antigen. 5) It is possible that these deleted genes are key to establishing specificity between B. adolescentis and its host.…”

mentioning

confidence: 99%

Intra-Species Diversity between SevenBifidobacterium adolescentisStrains Identified by Genome-Wide Tiling Array Analysis

Yasui

Tabata

Yamada

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…This difference in stability may be a product of mutual relationships between bacteria essential to liberate or use fucosylated ABH blood-group antigens from the mucosal surfaces (Rakoff-Nahoum et al, 2016), especially when we consider that several of the structurally important bacteria can be influenced by fucosylated glycans, e.g. , Barnesiella (Weiss et al, 2014), Lactobacillus (Uchida et al, 2006), Odoribacter (Hardham et al, 2008; Nagai et al, 2010), Helicobacter (Magalhaes et al, 2009), or Robinsoniella (Cotta et al, 2009; Shen et al, 2010). Robustness differences associated to genotype or breeding direction may have consequences for host fitness when considering the potential for dysbiosis after environmental disturbances.…”

Section: Discussionmentioning

confidence: 99%

Multigenerational Influences of the Fut2 Gene on the Dynamics of the Gut Microbiota in Mice

et al. 2017

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The FUT2 gene encodes an α-1,2-fucosyltransferase responsible for the expression of ABO histo-blood-group antigens on mucosal surfaces and bodily secretions. Individuals who carry at least one functional allele are known as “secretors,” whereas those homozygous for loss-of-function mutations are known as “non-secretors.” Non-secretor individuals are more susceptible to chronic inflammatory disorders such as Crohn’s Disease, which may be mediated by alterations in the microbiota. Here, we investigated the dynamics of microbial community assembly with respect to genotype using a Fut2-deficient mouse model, taking the genotype of the maternal lineage over two generations into account. We found strong differences in community assembly of microbial communities over time, depending on the Fut2 genotype of the host and that of their progenitors. By applying network analyses, we further identified patterns of specialization and stabilization over time, which are influenced by the host and parental genotype during the process of community development. We also show genotype- and breeding-dependent patterns of community susceptibility to disturbance in a novel in silico approach integrating ecological- and network analysis. Our results indicate that it may be important to investigate the influence of Fut2 genotype in a familial context in order to fully understand its role in the etiology of chronic inflammatory disorders.

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Lactic Acid Bacteria (LAB) Bind to Human B- or H-Antigens Expressed on Intestinal Mucosa

Cited by 30 publications

References 7 publications

Noroviral P-Particles as an In Vitro Model to Assess the Interactions of Noroviruses with Probiotics

Noroviral P-Particles as an In Vitro Model to Assess the Interactions of Noroviruses with Probiotics

Intra-Species Diversity between SevenBifidobacterium adolescentisStrains Identified by Genome-Wide Tiling Array Analysis

Multigenerational Influences of the Fut2 Gene on the Dynamics of the Gut Microbiota in Mice

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