Lacticaseibacillus rhamnosus: A Suitable Candidate for the Construction of Novel Bioengineered Probiotic Strains for Targeted Pathogen Control

Mathipa-Mdakane, Moloko G.; Thantsha, Mapitsi Silvester

doi:10.3390/foods11060785

Cited by 36 publications

(16 citation statements)

References 238 publications

(314 reference statements)

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“…In addition, some yeasts like Saccharomyces boulardii and Saccharomyces cerevisiae also have potential probiotic characteristics (Attri et al, 2021; Min et al, 2019). The Lactocaseibacillus rhamnosus strain GG is a potential and well‐known probiotic strain have better resistance to bile acid and survival capacity in the gastrointestinal tract (Mathipa‐Mdakane & Thantsha, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Development of white finger millet probiotic beverage using Lactocaseibacillus rhamnosus (LGG): Process optimization and study of physicochemical and nutritional properties

Buvaneswaran

Sunil

et al. 2022

J Food Process Engineering

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White finger millet (WFM) is a repository of vital nutrients and minerals. This study aims to develop the white finger probiotic beverage, optimize the ingredients level for the formulation, and study the physiochemical and nutritional properties of the formulated vegan WFM probiotic beverage (WFMPB). The optimum condition for the development of WFMPB was obtained by Box–Behnken design. The independent variable like WFM flour (10–14% w/v), microbial inoculum (1–5% v/v), and sugar (5–15% w/v), and their impact on the response variables like total solid content (TSS), pH, microbial count, and viscosity were studied for optimization. Increment in the amounts of WFM flour and inoculum levels had a significant impact on the microbial load and pH. However, sugar levels affected viscosity and TSS. The combination of 14% WFM, 1% microbial inoculum, and 5% sugar showed maximum desirability of 0.975, with the highest sensory score (8.34), TSS of 15.4 °Brix, pH of 4.33, the microbial load of 9.45 log (cfu/ml) and viscosity of 43.32 cP. WFMPB consists of 11.91% carbohydrates, 1.46% protein, 0.89% fat, and 0.632% crude fiber. The rheological studies showed that WFMPB is a shear‐thinning fluid (n ˂ 0.1). The study revealed that WFM is the better carrier matrix for the LGG growth and can be an alternative for the strict vegan and lactose‐intolerant consumers.

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Section: Introductionmentioning

confidence: 99%

“…The Lactocaseibacillus rhamnosus strain GG is a potential and wellknown probiotic strain have better resistance to bile acid and survival capacity in the gastrointestinal tract (Mathipa-Mdakane & Thantsha, 2022).…”

mentioning

confidence: 99%

Development of white finger millet probiotic beverage using Lactocaseibacillus rhamnosus (LGG): Process optimization and study of physicochemical and nutritional properties

Buvaneswaran

Sunil

et al. 2022

J Food Process Engineering

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“…strains tested for adherence to Caco-2 cells, the greatest adhesiveness was observed by L. paragasseri LDD-C1 and L. crispatus LCR-A21. The adhesion efficiency for these strains was greater than the reference strain LGG, which is considered highly adhesive [ 16 , 38 , 39 , 40 ]. The lower adhesive capacity of LGG compared to other commercial and potential probiotic bacteria has been previously reported [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Listeria monocytogenes Growth, Adherence and Invasion in Caco-2 Cells by Potential Probiotic Lactic Acid Bacteria Isolated from Fecal Samples of Healthy Neonates

et al. 2023

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Lactic acid bacteria (LAB) isolated from healthy humans may prove an effective tool against pathogen growth, adherence and invasion in intestinal epithelial cells. This study aimed to evaluate the antilisterial properties of LAB isolated from fecal samples of healthy neonates. Forty-five LAB strains were tested for their antimicrobial activity against ten Listeria monocytogenes strains with spot-on-lawn and agar-well diffusion assays, and ten lactobacilli strains were further assessed for their inhibitory effect against adherence and invasion of Caco-2 cells by L. monocytogenes EGDe. Inhibition was estimated in competition, exclusion or displacement assays, where lactobacilli and L. monocytogenes were added to Caco-2 monolayers simultaneously or 1 h apart from each other. Inhibition of L. monocytogenes growth was only displayed with the spot-on-lawn assay; cell-free supernatants of lactobacilli were not effective against the pathogen. Lactobacillus (L.) paragasseri LDD-C1 and L. crispatus LCR-A21 were able to adhere to Caco-2 cells at significantly higher levels than the reference strain L. rhamnosus GG. The adherence of L. monocytogenes to Caco-2 cells was reduced by 20.8% to 62.1% and invasion by 33.5% to 63.1% during competition, which was more effective compared to the exclusion and displacement assays. These findings demonstrate that lactobacilli isolated from neonatal feces could be considered a good candidate against L. monocytogenes.

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“…One of the shortcomings of wild-type probiotics is their non-specificity. However, a given probiotic could be engineered to exhibit species-specific inhibition of the pathogen and its associated infection [64]. The long-term stability of lactic acid bacteria starters for probiotics production during extended space missions could be problematic because of the impact of radiation [93].…”

Section: Study Of the Metagenome Of The Kmc And The Genomes Of Komaga...mentioning

confidence: 99%

The Conceptual Approach to the Use of Postbiotics Based on Bacterial Membrane Nanovesicles for Prophylaxis of Astronauts’ Health Disorders

ORLOVSKA¹,

PODOLICH²,

KUKHARENKO³

et al. 2022

Kosm. nauka tehnol.

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The functional fermented foods containing live microorganisms and their components are necessary for the normal functioning of the human body as normal gut microbiota needs fuel from external microbial organisms and their nanostructures — membrane vesicles (MVs), excreting outside. The сoncept that MVs may contribute to astronauts’ health probably to the same extent as their parental microbial cells do and be a temporary substitute for living microbial cells until we know more about the behavior of microbes in the space environment. The advantage of MVs is that they are not alive and cannot be changed under unfavorable conditions as microbial organisms may be. As the model, we selected MVs of a robust to environmental factors kombucha multimicrobial culture (KMC), known for its health-promoting characteristics for humans. We exposed KMC on the International Space Station in a hybrid space/Mars-like environment for an initial proof-of-concept stage. In the exposure study, KMC has survived a long-term period in harsh conditions, and the MVs generated by post-flight kombucha community members did not acquire toxicity, despite the changed membrane composition in the environment imitated conditions on the Mars surface. This observation, together with our KMC metagenomic and comparative genomic analyses of the dominant KMC bacterium Komagataeibacter oboediens, showed that the ground reference sample and spaceexposed ones were similar in topology and maintained their stability. In the next stage, we assessed the fitness, safety, and biodistribution of MVs of post-flight K. oboediens and showed that they were altered, but the modifications in membrane structure did not result in toxicity acquisition. Our proof-of-concept strategy is discussed in this review in line with the literature.

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Lacticaseibacillus rhamnosus: A Suitable Candidate for the Construction of Novel Bioengineered Probiotic Strains for Targeted Pathogen Control

Cited by 36 publications

References 238 publications

Development of white finger millet probiotic beverage using Lactocaseibacillus rhamnosus (LGG): Process optimization and study of physicochemical and nutritional properties

Development of white finger millet probiotic beverage using Lactocaseibacillus rhamnosus (LGG): Process optimization and study of physicochemical and nutritional properties

Inhibition of Listeria monocytogenes Growth, Adherence and Invasion in Caco-2 Cells by Potential Probiotic Lactic Acid Bacteria Isolated from Fecal Samples of Healthy Neonates

The Conceptual Approach to the Use of Postbiotics Based on Bacterial Membrane Nanovesicles for Prophylaxis of Astronauts’ Health Disorders

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