Lactiplantibacillus plantarum 299v (LP299V®): three decades of research

Abstract

This review aims to provide a comprehensive overview of the in vitro, animal, and clinical studies with the bacterial strain Lactiplantibacillus plantarum 299v (L. plantarum 299v; formerly named Lactobacillus plantarum 299v) published up until June 30, 2020. L. plantarum 299v is the most documented L. plantarum strain in the world, described in over 170 scientific publications out of which more than 60 are human clinical studies. The genome sequence of L. plantarum 299v has been determined and is available in the public domain (GenBank Accession number: NZ_LEAV01000004). The probiotic strain L. plantarum 299v was isolated from healthy human intestinal mucosa three decades ago by scientists at Lund University, Sweden. Thirty years later, a wealth of data coming from in vitro, animal, and clinical studies exist, showing benefits primarily for gastrointestinal health, such as reduced flatulence and abdominal pain in patients with irritable bowel syndrome (IBS). Moreover, several clinical studies have shown positive effects of L. plantarum 299v on iron absorption and more recently also on iron status. L. plantarum 299v is safe for human consumption and does not confer antibiotic resistance. It survives the harsh conditions of the human gastrointestinal tract, adheres to mannose residues on the intestinal epithelial cells and has in some cases been re-isolated more than ten days after administration ceased. Besides studying health benefits, research groups around the globe have investigated L. plantarum 299v in a range of applications and processes. L. plantarum 299v is used in many different food applications as well as in various dietary supplements. In a freeze-dried format, L. plantarum 299v is robust and stable at room temperature, enabling long shelf-lives of consumer healthcare products such as capsules, tablets, or powder sachets. The strain is patent protected for a wide range of indications and applications worldwide as well as trademarked as LP299V®.

Whole-Genome Sequencing, Phylogenetic and Genomic Analysis of Lactiplantibacillus pentosus L33, a Potential Probiotic Strain Isolated From Fermented Sausages

“…A neighbor-joining phylogenetic tree, including 1,000 bootstrap replications, based on orthologous gene clusters, was built to reveal the exact phylogenetic position of L. pentosus L33 within L. pentosus species ( Figure 2 ). Moreover, we have used 26 L. pentosus strains, 2 well documented L. plantarum probiotic strains; L. plantarum WCFS1 ( van den Nieuwboer et al, 2016 ) and L. plantarum 299v ( Nordström et al, 2021 ), as well as Staphylococcus aureus NCTC8325 and Streptococcus pneumoniae NCTC11032, as controls ( Supplementary Figure 1 ). The closest evolutionary relatives of L. pentosus L33 are L. pentosus IG7, which was isolated from the brine of natural Spanish-style green olive fermentation ( Calero-Delgado et al, 2018 ), and L. pentosus BGM48, originated from laboratory scale Sicilian-style green olive fermentation ( Golomb et al, 2013 ; Figure 2 ).…”

Effect of Oral Intake of Lactiplantibacillus plantarum APsulloc 331261 (GTB1TM) on Diarrhea-Predominant Irritable Bowel Syndrome: A Randomized, Double-Blind, Placebo-Controlled Study

“…The present work also revealed a correlation between significant increase in Lactobacillus abundance and improvement in IBS-D symptoms. Both the present and past studies indicated the beneficial effects of Lactobacillus plantarum ingestion in patients with IBS-D [ 70 ]. The results of the study revealed that GTB1 was efficacious as a probiotic for the improvement of symptoms in patients with IBS-D. Based on the results of the present work, the efficacy of GTB1 ingestion on other IBS subtypes will be investigated in future research.…”

Genomic and Phylogenetic Analysis of Lactiplantibacillus plantarum L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells

“…The phylogenetic tree is based on orthologous gene clusters and consists of L. plantarum L125 and 21 other potential probiotic L. plantarum strains. Among them, two well-established L. plantarum probiotic strains, L. plantarum WCFCS1 and L. plantarum 299v, were also included [ 6 , 42 ] ( Figure 2 ). To assure the accurate phylogenetic placement of the newly sequenced strain, Streptococcus pneumoniae Hu17 and Leuconostoc mesenteroides SRCM102733 have been used as outgroups/controls ( Figure S1 ).…”

“…In association with the host, L. plantarum strains have been found to attach to and transiently colonize the gut. Several strains can adhere directly onto the intestinal epithelium or mucins, using adhesins and adhesin-like molecules of their cellular surface, in chicken [ 4 ], murine [ 5 ] and human gut [ 6 ]. Persistence in the gastrointestinal (GI) tract is a prerequisite for these interactions to occur, and thus several in vitro and in vivo studies investigated the stress tolerance of novel strains [ 7 ].…”