Lactobacilli as live vaccine delivery vectors: progress and prospects

Seegers, Jos F. M. L.

doi:10.1016/s0167-7799(02)02075-9

Cited by 212 publications

(162 citation statements)

References 62 publications

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“…Gilliland, 1990;Goldin, 1998;Vaughan et al, 1999;Gorbach, 2000;Rolfe, 2000;Mercenier et al, 2003). Also, in recent years, selected Lactobacillus species have been used as vaccine delivery systems, which can be administered orally and can effectively induce protective immune responses (Mercenier et al, 1996;Wells et al, 1996;Pouwels et al, 2001;Seegers, 2002;Scheppler et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons

2005

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The combination of PFGE and hybridization approaches was used to study the genome of Lactobacillus acidophilus neotype strain ATCC 4356. PFGE analysis of chromosomal DNA after digestion with each of the rare-cutting restriction enzymes I-CeuI, NotI, CspI, SmaI, ApaI and SgrAI allowed the size of the circular chromosome of L. acidophilus to be estimated at 2?061 Mbp. The physical map contained 86 restriction sites for the six enzymes employed, with intervals between the sites varying from 1 to 88 kbp (~0?05-4?3 % of the chromosome). Based on the physical map, a genetic map was constructed via Southern blot analyses of L. acidophilus DNA using specific gene probes. A total of 73 probes representing key genes, including 12 rRNA (rrn) genes, were positioned on the latter map. Mapping analysis also indicated the presence of four rrn operons (rrnA-D) on the chromosome, each containing a single copy of each of the three rrn genes 16S (rrl ), 23S (rrs) and 5S (rrf ). Operon rrnD was inverted in orientation with respect to the others and contained a long 16S-23S intergenic spacer region with tRNA Ile and tRNA Ala genes, whereas the other operons contained a short spacer lacking any tRNA genes. The high-resolution physical/genetic map constructed in this study provides a platform for genomic and genetic studies of Lactobacillus species and for improving industrial and probiotic strains.

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Section: Introductionmentioning

confidence: 99%

Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons

2005

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“…As lactobacilli are commensals of the gut and are generally regarded as safe (GRAS-status), lactobacilli are good candidates for use as production and delivery hosts of heterologous proteins in food but also health care products. Genetically engineered lactobacilli expressing bacterial or viral proteins on the cell surface can potentially be used as oral vaccines [1,2]. On the other hand, Lactobacillus recombinants secreting tolerogenic autoantigens can be used to induce systemic peripheral T-cell tolerance by oral administration for treatment of autoimmune disease [1].…”

Section: Introductionmentioning

confidence: 99%

Growth phase of orally administered Lactobacillus strains differentially affects IgG1/IgG2a ratio for soluble antigens: implications for vaccine development

Maassen

Boersma²,

Holten-Neelen³

et al. 2003

Vaccine

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Lactobacillus strains with probiotic activity are major constituents of numerous common food products. Due to their 'generally regarded as safe'-status (GRAS-status), Lactobacillus strains can also be genetically engineered for use in oral immunotherapeutic applications, such as vaccination and T lymphocyte tolerance induction in autoimmune disease.In the current study, we demonstrate that the growth phase of orally administered individual Lactobacillus strains can differentially affect antigen-specific antibody subclasses IgG1 and IgG2a, which might reflect skewing of systemic activity of T helper cell type 2 (Th2) and T helper cell type 1 (Th1) pathways, respectively. Mice were orally fed different wild type Lactobacillus strains in log phase or stationary phase and immunized intraperitoneally with a T-cell dependent protein antigen. Sera were evaluated for the ratio of antigen-specific IgG1 and IgG2a antibodies. Stationary Lactobacillus murines and Lactobacillus casei cultures, but not two other Lactobacillus strains, evoked significantly higher IgG1/IgG2a ratios than log phase cultures, possibly relating to increased activity of the Th2-pathway. Despite normal variation in antibody responses against TNP-CGG among individual mice, a high correlation was found between the IgG1 and IgG2a responses of mice within experimental groups. This differential antibody response is likely due to growth phase-dependent differences in bacterial cell composition.Since Lactobacillus growth phase dependent skewing of antibody responses possibly reflecting T-cell pathways can inadvertently affect allergic and (auto)-immune responses, the current findings strongly caution against unidimensional views on the oral administration of individual Lactobacillus strains for probiotic or immunotherapeutic purposes, but also suggest additional possibilities for immune modulation.

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“…In this sense, some scientists are considering the possibility of combining these benefits using genetic engineering (Steidler, 2003;Abbot, 2004). There are evidences that some genetically-modified bacteria may be efficient vehicles for drugs, vitamins, or vaccines, such as genetically-modified Lactococcus lactis that may secrete medicines to fight intestinal disorders (Seegers, 2002;Steidler et al, 2000;Steidler, 2003;Charng et al, 2006).…”

Section: Substrates and Bacterial Metabolitesmentioning

confidence: 99%

Probiosis: concepts and prospects

Barbosa

Figueiredo-Lima

Givisiez

et al. 2010

Rev. Bras. Cienc. Avic.

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The use of probiotics in animal and human feeding has been a subject of increasing interest both for the academia and the industry, mostly due to their potential positive effects on health and profitability. However, the knowledge on the composition of bacterial gastrointestinal communities in humans and animals, as well as its respective nutritional requirements, is far from being elucidated. Due to the ban of the use of antibiotic growth promoters in animal feeds, there has been an increasing interest on the utilization of probiotics to improve animal intestinal health under commercial settings. However, the possibility of horizontal transference of antibiotic-resistant genes between probiotic bacteria and pathogenic species has become a concern of poultry farmers and consumers around the world. Innovative ideas have emerged, such as the addition of essential oils, spices, and other plant extracts to feeds of monogastric animals to promote intestinal health. These natural compounds are considered ecologically adequate and safe for feeding purposes. This new reality will probably change the direction of research and of the use of additives in poultry production.

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Lactobacilli as live vaccine delivery vectors: progress and prospects

Cited by 212 publications

References 62 publications

Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons

Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons

Growth phase of orally administered Lactobacillus strains differentially affects IgG1/IgG2a ratio for soluble antigens: implications for vaccine development

Probiosis: concepts and prospects

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