Lactobacilli Supplemented with Larch Arabinogalactan and Colostrum Stimulates an Immune Response towards Peripheral NK Activation and Gut Tolerance

Velikova, Tsvetelina; Tumangelova-Yuzeir, Kalina; Georgieva, Ralitsa; Ivanova-Todorova, Ekaterina; Karaivanova, E.; Nakov, Ventsislav; Nakov, Radislav; Kyurkchiev, Dobroslav

doi:10.3390/nu12061706

Cited by 12 publications

(2 citation statements)

References 35 publications

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“…More detailed studies are required to determine which ones play a dominant role in IBS. We also discovered that plasma cell infiltration levels, CD8 + T cells, memory B cells, M0 macrophages, and activated natural killer (NK) cells reduced significantly in IBS patients, which is partly consistent with existing findings 32 , 39 , 40 . These may be due to the different sample sizes and different segments of intestinal biopsy in the various studies.…”

Section: Discussionsupporting

confidence: 91%

Downregulated APOD and FCGR2A correlates with immune infiltration and lipid-induced symptoms of irritable bowel syndrome

Ran,

Wu,

et al. 2023

Sci Rep

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Fat intake is among the most significant triggers for symptom development in patients with irritable bowel syndrome (IBS). Nevertheless, long-term restriction in fatty foods ingestion may lead to nutritional inadequacies. This study aimed to identify the crucial genes involved in lipid-induced gastrointestinal symptoms, contributing to helping IBS patients regulate fat. The clinical characteristics of the subjects were collected by questionnaire investigation and analyzed using multivariate logistic regression. Differentially expressed genes (DEG) and signaling pathways were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. ImmuInfiltration and CIBERSORT packages evaluated small intestine immune cell infiltration. Random forest and SVM-RFE algorithms were used to select hub genes. A receiver operating characteristic curve was used to access the diagnostic significance of each hub gene. Gene Set Enrichment Analysis (GSEA) was performed to identify hub genes’ molecular processes in IBS development after lipid infusion. IBS patients’ risk, severity, and quality of life increased with fat intake. In total, 116 robust DEGs were identified in IBS patients after lipid infusion using the GSE166869 dataset and were mainly clustered in the immune and inflammatory pathways. IBS patients had greater Neutrophils, CD4+ T cells, and M1 Macrophages than healthy controls. Furthermore, infiltration levels of Neutrophils and resting memory CD4+ T cells were inversely related to the expression of hub genes (IGKV1D-43, IGKV1-12, APOD, FCGR2A and IGKV2-29). After lipid infusion, GSEA results of each hub gene indicated the relevance of proinflammatory pathways in IBS pathogenesis. After verification, only APOD and FCGR2A were stably downregulated in small intestinal mucosa and plasma of IBS patients. The area under the curve of APOD combined with FCGR2A expression was 0.9. APOD and FCGR2A may be promising biomarkers for IBS diagnosis and lipid-sensitive IBS patients. Their potential roles in the immune microenvironment of the small intestinal mucosa may provide a vital clue to IBS precision therapy.

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Section: Discussionsupporting

confidence: 91%

Downregulated APOD and FCGR2A correlates with immune infiltration and lipid-induced symptoms of irritable bowel syndrome

Ran,

Wu,

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…More detailed studies are required to determine which ones play a dominant role in IBS. We also found that in ltration levels of plasma cells, CD8 + T cells, memory B cells, M0 macrophages, and activated natural killer (NK) decreased markedly in patients with IBS, which is partly consistent with published studies (27,34,35). These may be due to the different sample sizes and different segments of intestinal biopsy in the various studies.…”

Section: Discussionsupporting

confidence: 89%

Identification of biomarkers functioning in the lipid-induced IBS symptoms

Ran

et al. 2023

Preprint

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Background: Fat intake is one of the most important triggers for symptom development in patients with irritable bowel syndrome (IBS). But long-term restriction in fatty foods ingestion may lead to nutritional inadequacies. In this study, we aim to identify the vital genes that function in lipid-induced gastrointestinal symptoms, contributing to precise management of fat control for IBS patients. Methods: The clinical characteristics of the subjects were collected by questionnaire investigation and analyzed using multivariate logistic regression. Differentially expressed genes (DEG) and signaling pathways were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The infiltrated immune cells in the small intestinal mucosa were analyzed using ImmuInfiltration and CIBERSORT packages. Random forest and SVM-RFE algorithms were used to select hub genes. A receiver operating characteristic curve (ROC) was performed to evaluate the diagnostic significance of each hub gene. Gene Set Enrichment Analysis (GSEA) was conducted to clarify the possible molecular mechanisms of hub genes in the pathogenesis of IBS after lipid infusion. Results: Fat intake was positively associated with the risk, severity, and quality of life (QOL) of IBS patients. A total of 116 robust DEGs were identified in patients with IBS after lipid infusion using the GSE166869 dataset and were mainly clustered in the immune and inflammatory pathways. The infiltration levels of Neutrophils, CD4+T cells and M1 Macrophages were significantly higher in IBS patients compared to the healthy controls. Furthermore, infiltration levels of Neutrophils and resting memory CD4+ T cells were inversely related to the expression of hub genes (IGKV1D-43, IGKV1-12, APOD, FCGR2A and IGKV2-29). GSEA results of each hub gene suggested the importance of pro-inflammatory pathways in IBS pathogenesis after lipid infusion. After verification, only APOD and FCGR2A were stably down-regulated in both small intestinal mucosa and plasma of IBS patients. The area under the curve of APOD combined with FCGR2A expression was 0.9. Conclusions: APOD and FCGR2A may act as promising biomarkers for discriminating lipid-sensitive IBS patients and IBS diagnosis. Their potential roles in the immune microenvironment of the small intestinal mucosa may provide a vital clue to IBS precision therapy.

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