Lactobacillus brevis  T2102 suppresses the growth of colorectal cancer cells by activating  SIRT1

Harada, Gakuro; Pattarawat, Pawat; Ito, Kenji; Mutoh, T.; Hasegawa, Takanori; Katakura, Yoshinori

doi:10.1016/j.jff.2016.01.016

Cited by 13 publications

(14 citation statements)

References 37 publications

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“…Previously, we established a system for screening foods/food ingredients that augment the SIRT1 promoter in Caco-2 cells and identified Lactobacillus brevis T2102 as SIRT1 -activating lactic acid bacterium [26]. Here, we established a similar system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells, where pSIRT3p-EGFP was transduced.…”

Section: Resultsmentioning

confidence: 99%

Pomegranate‐Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3‐Mediated SOD2 Activation

Zhao

Sakaguchi

Fujita

et al. 2016

Oxidative Medicine and Cellular Longevity

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Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A.

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Section: Resultsmentioning

confidence: 99%

Pomegranate‐Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3‐Mediated SOD2 Activation

Zhao

Sakaguchi

Fujita

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…A reporter containing TCF/LEF elements (M50 Super 8 × TOPFlash) (Veeman et al, 2003) (Addgene, Cambridge, MA, United States) was used as the reporter vector (Harada et al, 2016). The luciferase assay was performed using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, United States), as described previously (Yamashita et al, 2014).…”

Section: Luciferase Assaymentioning

confidence: 99%

“…The CMV promoter region of the EGFP expression vector was replaced with the promoter of the gene of interest and introduced into tissue-derived cell lines. These recombinant cells were treated with food components, polyphenols, and compounds, and changes in EGFP fluorescence were monitored using an imaging cytometer (IN Cell Analyzer 1000) (Harada et al, 2016;Zhao et al, 2016;Chong et al, 2019). We successfully identified promoter-activating polyphenols and lactic acid bacteria.…”

Section: Introductionmentioning

confidence: 99%

Fisetin Promotes Hair Growth by Augmenting TERT Expression

Kubo

Ogawa

Uehara

et al. 2020

Front. Cell Dev. Biol.

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Although thinning hair and alopecia are not recognized as severe diseases, hair loss has implications for mental health and quality of life; therefore, a large number of studies have been carried out to develop novel hair growth agents. In the present study, we aimed to examine the potential of telomerase reverse transcriptase (TERT), because TERT overexpression in skin activates resting hair follicle bulge stem cells, which triggers initiation of a new hair follicle growth phase and promotes hair synthesis. To this end, we screened polyphenols that activate TERT expression in keratinocytes, and identified resveratrol and fisetin as strong hTERT-augmenting compounds. These polyphenols also regulated the gene expression of cytokines such as IGF-1 and KGF, which activate the β-catenin pathway, and TGF-β1, which plays an important role in maintaining the niche of hair follicle stem cells, thus are thought to play roles in promoting hair growth. We additionally showed that these polyphenols, especially fisetin, promoted hair growth from the shaved dorsal skin of mice, which suggests that these polyphenols activate the transition from telogen to anagen phase. Histological studies indicated that the dorsal skin of mice treated with these polyphenols contained numerous hair follicles and was thickened compared with that in control mice. Furthermore, on the dorsal skin of mice treated with resveratrol and fisetin, a number of proliferating cells (Ki67 + cells) were observed around the hair papilla. These results suggest that resveratrol and fisetin induce a shift from telogen to anagen in the hair follicle by inducing proliferation of hair follicle bulge stem cells, thus promoting hair growth.

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“…We amplified the human SIRT1 promoter (-1593 to -1) by PCR using human genomic DNA as the template; the amplified fragment was cloned into the promoter-less pEGFP-C3 (Takara, Shiga, Japan) and designated pSIRT1p-EGFP (Harada et al 2016).…”

Section: Screening System For Foods That Activate the Sirt1 Promotermentioning

confidence: 99%

“…Previously, we established a system for screening foods/food ingredients that augment SIRT1 and SIRT3 expression in Caco-2 cells, and identified Lactobacillus brevis T2102 and pomegraniin A as SIRT1-and SIRT3-activating food components (Zhao et al 2016;Harada et al 2016). Here, we established a similar system for screening foods/food ingredients that activate SIRT1 expression using HaCaT cells that express EGFP under the control of the SIRT1 promoter.…”

Section: Identification Of Mushrooms That Augment Sirt1 Transcriptionmentioning

confidence: 99%

Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes

et al. 2018

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Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

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Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1

Cited by 13 publications

References 37 publications

Pomegranate‐Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3‐Mediated SOD2 Activation

Pomegranate‐Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3‐Mediated SOD2 Activation

Fisetin Promotes Hair Growth by Augmenting TERT Expression

Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes

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