Lactobacillus casei loaded Soy Protein-Alginate Microparticles prepared by Spray-Drying

Abstract

SummaryThis article presents a novel formulation for preparation of Lactobacillus casei 01 encapsulated in soy protein isolate and alginate microparticles using spray drying method. A response surface methodology was used to optimise the formulation and the central composite face-centered design was applied to study the effects of critical material attributes and process parameters on viability of the probiotic after microencapsulation and in simulated gastrointestinal conditions. Spherical microparticles were produced in high yield (64 %), narrow size distribution (d 50 =9.7 μm, span=0.47) and favourable mucoadhesive properties, with viability of the probiotic of 11.67, 10.05, 9.47 and 9.20 log CFU/g after microencapsulation, 3 h in simulated gastric and intestinal conditions and four-month cold storage, respectively. Fourier-transform infrared spectroscopy confirmed the probiotic stability after microencapsulation, while differential scanning calorimetry and thermogravimetry pointed to high thermal stability of the soy protein isolate-alginate microparticles with encapsulated probiotic. These favourable properties of the probiotic microparticles make them suitable for incorporation into functional food or pharmaceutical products.

Tablets of soy protein-alginate microparticles with Lactobacillus casei 01: Physicochemical and biopharmaceutical properties

“…The same result was observed with the actual L. casei 01 loaded in SPI-ALG MPs. The survival rate in the MPs was maintained nearly 80% after four-month cold storage, decreasing by 2 log units [20]. However, when the tablets of the probiotic SPI-ALG MPs were stored at 25 °C/60% RH, the survival rate of L. casei 01 after 42 days was between 63 (series I and IV) and 76% (series II), decreasing by averagely 3 log units, as pointed out.…”

“…Full physicochemical and biological characterization of the prepared probiotic loaded MPs was conducted and the data are presented in Hadzieva et al [20]. Before their incorporation into tablet dosage form, MPs were characterized in term of their flowability and compressibility.…”

“…The probiotic loaded SPI-ALG MPs obtained from the optimal formulation had a spherical shape but a disk shape as well, showing low level of humidity (0.8±0.1%), solubility of approx. 95.0±0.2%, wettability of 100% in 5 min, as well as swelling and mucoadhesion properties, and in vitro release profile suitable for colon delivery [20].…”

“…In this study, direct-compress-tablets of previously optimized Lactobacillus casei 01 loaded soy protein-alginate MPs (SPI-ALG MPs) [20] were prepared. In preparation of probiotic loaded MPs, ALG has been used as a protective polymeric carrier due to its confirmed favorable properties, including biocompatibility, biodegradability, safety, and ease of gelation in the presence of divalent cations.…”

“…SPI served as a cell adhesion ligand to increase the cell loading, enhance mechanical properties of the MPs and thus, to preserve viability of the probiotic at low pH. As added values, biodegradability, low immunogenicity, and similarity with the components of the tissue extracellular matrix of SPI were considered [20]. For tablet preparation, different matrix forming excipients were applied and their effects on resistance of probiotic to simulated gastrointestinal conditions and storage stability were evaluated.…”