Lactobacillus-dominance and rapid stabilization of vaginal microbiota in combined oral contraceptive pill users examined through a longitudinal cohort study with frequent vaginal sampling over two years

Tuddenham, Susan; Gajer, Pawel; Burke, Anne E.; Murphy, Catherine C.; Klein, Sabra L.; Stennett, Christina A.; Wilgus, Barbara; Ravel, Jacques; Ghanem, Khalil G.; Brotman, Rebecca M.

doi:10.1016/j.ebiom.2022.104407

Cited by 12 publications

(1 citation statement)

References 70 publications

(109 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An effective treatment to maintain a vaginal microbiota dominated by Lactobacillus, beneficial for in vitro fertilization (IVF) treatments, involves maintaining stable estrogen levels. An association between Haemophilus and endometriosis was observed, along with its potential relationship with estrogen levels and hormonal treatment [66,67]. On the other hand, Haemophilus influenzae has been associated with the etiology of periodontitis [68], a condition frequently found in patients with endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Distinct Gastrointestinal and Reproductive Microbial Patterns in Female Holobiont of Infertility

Marcos,

Rus,

Areal-Quecuty

et al. 2024

Microorganisms

View full text Add to dashboard Cite

The microbiota is in symbiosis with the human body as a holobiont. Infertility conditions affect the female reproductive tract (FRT) and its resident microbiota. However, a disturbance in homeostasis could influence the FRT and other distal body sites, such as the gastrointestinal tract (GIT). We included 21 patients with endometriosis and other infertility-associated diseases with clinical profiles and biological samples from the FRT (endometrium, endometrial fluid, and vagina), and GIT samples (oral and feces). We performed a 16S rRNA analysis of site-specific microbial communities and estimated diversity metrics. The study found body site-specific microbial patterns in the FRT–GIT. In both study groups, Lactobacillus was the most shared Amplicon Sequence Variant (ASV), a precise identifier of microbial sequences, between endometrial and vagina samples. However, shared Gardnerella and Enterobacteriaceae ASVs were linked to other conditions but not endometriosis. Remarkably, Haemophilus was a specific GIT-shared taxon in endometriosis cases. In conclusion, infertility influences distinctly the FRT and GIT microbiomes, with endometriosis showing unique microbial characteristics. We proposed the concept of ‘female holobiont’ as a community that comprises the host and microbes that must maintain overall homeostasis across all body sites to ensure a woman’s health. Insights into these microbial patterns not only advance our understanding of the pathophysiology of infertility but also open new avenues for developing microbe-based therapeutic interventions aimed at restoring microbial balance, thereby enhancing fertility prospects.

show abstract

Section: Discussionmentioning

confidence: 99%

Distinct Gastrointestinal and Reproductive Microbial Patterns in Female Holobiont of Infertility

Marcos,

Rus,

Areal-Quecuty

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Exploring the Clinical Signatures of Cervical Dysplasia Patients and Their Association With Vaginal Microbiota

Cheng,

Yan,

Yang

et al. 2024

Cancer Medicine

View full text Add to dashboard Cite

AimsThe vaginal microbiota plays a crucial role in women's health, and an imbalanced vaginal microbiota is linked to various diseases, including human papillomavirus (HPV) infection. However, most available data comes from Western countries and primarily focuses on HPV infection, with only a few studies considering detailed clinical factors to explore the relationship between vaginal microbiota and the development of cervical cancer, especially in China.Materials and MethodsOur study involved 266 women, including individuals at all stages of cervical dysplasia, and healthy controls with and without HPV infection. We assessed several aspects of the vaginal environment, including vaginal microbiota composition using 16S rRNA gene sequencing, HPV infection status using the standard Roche Cobas method, pH value, age, and H2O2 levels from clinical records, and partner numbers and contraceptive methods obtained through questionnaires. The association of these clinical signatures with cervical dysplasia stages and vaginal microbiota was analyzed.Key FindingsOur findings demonstrate a significant association between vaginal microbiota and cervical dysplasia stages. Patients with cervical dysplasia and cancer showed a substantial increase in HPV 16 infection, a higher prevalence of pH > 5, a lower H2O2 level, and older ages compared to healthy individuals. Additionally, these factors influence the beta diversity of the vaginal microbiota.SignificanceThese results underscore the importance of considering the vaginal microbiota within the cancer microenvironment and highlight the need to integrate all available data to aid in the current diagnosis and understanding of cervical dysplasia and the cervical cancer microenvironment.

show abstract

Vaginal microbiota stability over 18 months in young student women in France

Tamarelle,

Thiébaut,

de Barbeyrac

et al. 2024

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Lactobacillus-dominance and rapid stabilization of vaginal microbiota in combined oral contraceptive pill users examined through a longitudinal cohort study with frequent vaginal sampling over two years

Cited by 12 publications

References 70 publications

Distinct Gastrointestinal and Reproductive Microbial Patterns in Female Holobiont of Infertility

Distinct Gastrointestinal and Reproductive Microbial Patterns in Female Holobiont of Infertility

Exploring the Clinical Signatures of Cervical Dysplasia Patients and Their Association With Vaginal Microbiota

Vaginal microbiota stability over 18 months in young student women in France

Contact Info

Product

Resources

About