Lactobacillus Dominate in the Intestine of Atlantic Salmon Fed Dietary Probiotics

Gupta, S. R.; Fečkaninová, Adriána; Lokesh, Jep; Koščová, Jana; Sørensen, Mette; Fernandes, Jorge M. O.; Kiron, Viswanath

doi:10.3389/fmicb.2018.03247

Cited by 62 publications

(57 citation statements)

References 89 publications

(97 reference statements)

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“…In accordance with previous studies in Atlantic salmon (20,(33)(34)(35)(36)(37)(38), Aliivibrio, B. andersonii and Mycoplasma were identified as core microbiota in the present study. Aliivibrio is commonly found in the seawater phase Atlantic salmon intestine (35)(36)(37)(39)(40)(41)(42)(43) and has been identified as a core taxon of both wild and captive Atlantic salmon (34,36,37). Provided its common presence in seawater, Aliivibrio may have originated from the surrounding water and colonized the intestinal mucosa as Atlantic salmon constantly drink seawater to prevent dehydration in a hyperosmotic environment.…”

Section: Discussionsupporting

confidence: 93%

“…Our results corroborate previous findings in rainbow trout revealing that mucosa-associated intestinal microbiota was less influenced by dietary inclusion of 30% black soldier fly larvae meal compared to digesta-associated intestinal microbiota (28,29). Results from molecular-based studies on salmonid intestinal microbiota hitherto suggest that diet modulates digesta-and mucosa-associated intestinal microbiota to varying degrees with the latter generally being more resilient to dietary interventions (19,(26)(27)(28)(29)35). As such, current practices of profiling only one of or a mixture of digesta-and mucosa-associated microbiota may obscure the response of intestinal microbiota to dietary changes.…”

Section: Significant Interactions Between Diet and Sample Origin Effectsupporting

confidence: 90%

“…Though Spirochaetes has typically been found in low abundances in the Atlantic salmon intestine (19,23,27,39), two recent studies have identified B. andersonii as a core taxon of both digesta-and mucosa-associated intestinal microbiota in seawater phase Atlantic salmon (35,36). Furthermore, B. andersonii is also a predominant taxon in the digesta and mucosa in one of the studies (36).…”

Section: Discussionmentioning

confidence: 98%

“…This taxon has also been found in the intestine and gill tissue of rainbow trout (Oncorhynchus Mycoplasma is widely distributed in nature and well known for its minute size and lack of cell wall. Like Aliivibrio, Mycoplasma has been frequently identified as a core taxon of both wild and captive Atlantic salmon as well (20,33,(35)(36)(37)(38). It was found to be more abundant in marine adults than in freshwater juvenile Atlantic salmon (37) and sporadically predominate intestinal microbial community in the digesta (20,36,37,41,51) and mucosa (35) reaching as high as > 90% of total read counts in extreme cases.…”

Section: Discussionmentioning

confidence: 99%

“…Like Aliivibrio, Mycoplasma has been frequently identified as a core taxon of both wild and captive Atlantic salmon as well (20,33,(35)(36)(37)(38). It was found to be more abundant in marine adults than in freshwater juvenile Atlantic salmon (37) and sporadically predominate intestinal microbial community in the digesta (20,36,37,41,51) and mucosa (35) reaching as high as > 90% of total read counts in extreme cases. Due to its small compact genome and limited biosynthesis capacities, Mycoplasma typically forms obligate parasitic or commensal relationships with its host to obtain necessary nutrients such as amino acids, fatty acids and sterols (52).…”

Section: Discussionmentioning

confidence: 99%

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Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

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Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. In a 16-week seawater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially-relevant reference diet or an insect meal diet containing 15% black soldier fly (Hermetia illucens) larvae meal. The digesta-and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Regardless of diet, we observed substantial differences between digesta-and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Lastly, multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and unclassified Spirochaetaceae, associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Overall, our data clearly indicate that responses in digesta-and mucosa-associated microbiota to dietary inclusion of insect meal differ, with the latter being more resilient to dietary changes. Microbiota | Gut biogeography | Atlantic salmon | Diet | Black soldier fly Correspondence: yanxianl@nmbu.no Characterizing intestinal microbiota and its associationsLi & Bruni et al. | bioRχiv | May 24, 2020 | 1-8 with host responses is an essential step towards identifying key microbial clades promoting fish health and welfare. Ultimately, a milestone in the fish microbiota research would be knowing how to selectively manipulate the microbiota to improve the growth performance, disease resistance and health status of farmed fish. The main aims of the work presented herein were (i) to compare distal intestinal microbiota of Atlantic salmon fed a commercially relevant diet and an insect meal-based diet, (ii) to further explore the dissimilarity between digesta-and mucosa-associated microbiota and the differences in their response to dietary changes, and (iii) to identify associations between microbial clades and host responses. This work was part of a larger study consisting of a freshwater and seawater feeding trial that aimed to investigate the nutritional value and possible health effects for Atlantic salmon of a protein-rich insect meal produced from black soldier fly (Hermetia illucens) larvae. The present work focuses on the intestinal microbiota in seawater phase Atlantic salmon fed an insect meal diet containing 15% black soldier fly larvae meal for 16 weeks. Results on feed utilization, growth performance, fillet quality, intestinal histomorphology and gene expression have been reported elsewhere (30-32). ResultsHereafter, different sample groups are named based on the combination of die...

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