Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.

Abstract

Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterized Lactobacillus species, including L. acidophilus (ATCC 4356), L. brevis (ATCC 367), L. delbrueckii ssp. delbrueckii (ATCC 9645), L. fermentum (ATCC 23271), L. paracasei (ATCC 335), L. plantarum (ATCC 8014), and L. rhamnosus (ATCC 9595), against Candida albicans (ATCC 18804), Neisseria gonorrhoeae (ATCC 9826), and Streptococcus agalactiae (ATCC 13813). The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv) inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Tested lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. With the exception of L. acidophilus and L. paracasei, they adhered to HeLa cells. However, only L. fermentum produced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that all Lactobacillus strains inhibit C. albicans binding to mucin (p < 0.001), likely due to the production of substances with antimicrobial activity. Clinical isolates belonging to the most common Candida species associated to vaginal candidiasis were inhibited by L. fermentum. Collectively, our data suggest that L. fermentum ATCC 23271 is a potential probiotic candidate, particularly to complement candidiasis treatment, since presented with the best probiotic profile in comparison with the other tested lactobacilli strains.

Characterization of Lactic Acid Bacteria Isolated From the Gastrointestinal Tract of a Wild Boar as Potential Probiotics

“…Autoaggregation and coaggregation of probiotics play important roles in preventing pathogens from surface colonization (21). Autoaggregation can enable microorganisms which belong to the same species to join self-forming groups, and this phenomenon usually associated with microorganisms binding to intestinal mucosa (51).…”

“…LAB have widely been applied in animal feed. At present, screening for new probiotics, especially from some undeveloped species has become an ongoing practice (21).…”

Biological properties and production of bacteriocins‐like‐inhibitory substances by Lactobacillus sp. strains from human vagina

“…; Carmo et al . ). After aerobic incubation for 18–24 h at 37°C, the absorbance at 600 nm (OD600) (BioRad Microplate Reader Model 680, BioRad, Hercules, CA) was recorded and the culture broth was removed.…”

“…The assay for in vitro biofilm formation was performed in polystyrene 96-wells microplates, making 1Á5 9 10 5 CFU per ml inoculum of overnight broth culture (Roccasalva et al 2008;Carmo et al 2016). After aerobic incubation for 18-24 h at 37°C, the absorbance at 600 nm (OD600) (BioRad Microplate Reader Model 680, BioRad, Hercules, CA) was recorded and the culture broth was removed.…”

Antimicrobial Compounds Produced by Vaginal Lactobacillus crispatus Are Able to Strongly Inhibit Candida albicans Growth, Hyphal Formation and Regulate Virulence-related Gene Expressions

“…The modified agar overlay method was used to determine the antimicrobial activity of lactobacilli against C. albicans (do Carmo et al, 2016). Ten microliter of Lactobacillus spp.…”