Lactobacillus fermentum strains from human breast milk with probiotic properties and cholesterol-lowering effects

Asan-Ozusaglam, Meltem; Gunyakti, Ayse

doi:10.1007/s10068-018-0494-y

Cited by 45 publications

(18 citation statements)

References 31 publications

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“…Many of these bacteria may be human commensals or have potential probiotic effects [1]. Lactic acid bacteria, such as Lactobacillus fermentum, L. gasseri, L. rhamnosus, isolated from human breast milk, can be regarded as potential probiotic bacteria [2][3][4]. Previous studies have suggested that commensal coagulase-negative staphylococci and viridans streptococci found in breast milk can reduce the acquisition of undesired pathogens by infants exposed to hospital environments [5].…”

Section: Introductionmentioning

confidence: 99%

Changes in the Bacterial Diversity of Human Milk during Late Lactation Period (Weeks 21 to 48)

Marin-Gómez

Grande

Pulido

et al. 2020

Foods

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Breast milk from a single mother was collected during a 28-week lactation period. Bacterial diversity was studied by amplicon sequencing analysis of the V3-V4 variable region of the 16S rRNA gene. Firmicutes and Proteobacteria were the main phyla detected in the milk samples, followed by Actinobacteria and Bacteroidetes. The proportion of Firmicutes to Proteobacteria changed considerably depending on the sampling week. A total of 411 genera or higher taxons were detected in the set of samples. Genus Streptococcus was detected during the 28-week sampling period, at relative abundances between 2.0% and 68.8%, and it was the most abundant group in 14 of the samples. Carnobacterium and Lactobacillus had low relative abundances. At the genus level, bacterial diversity changed considerably at certain weeks within the studied period. The weeks or periods with lowest relative abundance of Streptococcus had more diverse bacterial compositions including genera belonging to Proteobacteria that were poorly represented in the rest of the samples.

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Section: Introductionmentioning

confidence: 99%

Changes in the Bacterial Diversity of Human Milk during Late Lactation Period (Weeks 21 to 48)

Marin-Gómez

Grande

Pulido

et al. 2020

Foods

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“…(2018) [25] observed 100% survivability for breast milk isolates even after 4h of exposure to one percent bile salt.…”

Section: Probiotic Properties Of the Isolate 321 Acid And Bile Tolerancementioning

confidence: 99%

Probiotic characterization of lactic acid bacteria isolated from human milk

Rappai¹,

Beena²,

James³

et al. 2020

Pharma Innovation

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The objective of this study was to evaluate the probiotic properties of lactic acid bacteria isolated from human milk. Based on biochemical characterization and 16S rRNA sequencing, the isolate was identified as Pediococcus pentosaceus DM101, the sequence of which is deposited in NCBI with accession number MK774704. Probiotic characterization in terms of acid tolerance, bile tolerance, adhesion potential, and safety of was done in vitro. Pediococcus pentosaceus DM101 was found to withstand pH 3.0 and 0.6% (w/v) bile salt for four hours. Auto-aggregation and cell surface hydrophobicity values of 68% and 60% signifies the good adhesion potential of the isolate. Inability to cause hemolysis and liquefy gelatin suggests the possible absence of virulence factors. Antibiogram revealed the isolate to be resistant to Vancomycin, Methicillin, Bacitracin, and Cephalosporin group of antibiotics. Exopolysaccharide producing nature of the isolate was confirmed by Congo red assay. DPPH assay of the cell free supernatant revealed an IC 50 value of 20.78mg/ml, endorsing its radical scavenging potential. The results obtained in this study decipher Pediococcus pentosaceus DM101 as a propitious candidate for designing foods to counteract oxidative stress proactively.

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“…Apart from L. reuteri , Lactobacillus fermentum strains from human milk have been studied as probiotic candidates, and proved to be safe, well tolerated and useful for the prophylaxis against community-acquired infections [ 171 ]. They also showed cholesterol-lowering effects in simulated models of liver and gastrointestinal tract [ 172 ]. Both L. fermentum , and another milk-derived species, Lactobacillus salivarius , enhanced both natural and acquired immune responses, via activation of natural killer and T cell subsets and induction of a broad array of cytokines in peripheral blood mononuclear cells in vitro [ 173 ].…”

Section: A Focused Insight Into Human Milk Microbiotamentioning

confidence: 99%

Human Milk Microbiota and Oligosaccharides: A Glimpse into Benefits, Diversity, and Correlations

Moubareck

2021

Nutrients

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Human milk represents a cornerstone for growth and development of infants, with extensive array of benefits. In addition to exceptionally nutritive and bioactive components, human milk encompasses a complex community of signature bacteria that helps establish infant gut microbiota, contributes to maturation of infant immune system, and competitively interferes with pathogens. Among bioactive constituents of milk, human milk oligosaccharides (HMOs) are particularly significant. These are non-digestible carbohydrates forming the third largest solid component in human milk. Valuable effects of HMOs include shaping intestinal microbiota, imparting antimicrobial effects, developing intestinal barrier, and modulating immune response. Moreover, recent investigations suggest correlations between HMOs and milk microbiota, with complex links possibly existing with environmental factors, genetics, geographical location, and other factors. In this review, and from a physiological and health implications perspective, milk benefits for newborns and mothers are highlighted. From a microbiological perspective, a focused insight into milk microbiota, including origins, diversity, benefits, and effect of maternal diet is presented. From a metabolic perspective, biochemical, physiological, and genetic significance of HMOs, and their probable relations to milk microbiota, are addressed. Ongoing research into mechanistic processes through which the rich biological assets of milk promote development, shaping of microbiota, and immunity is tackled.

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Lactobacillus fermentum strains from human breast milk with probiotic properties and cholesterol-lowering effects

Cited by 45 publications

References 31 publications

Changes in the Bacterial Diversity of Human Milk during Late Lactation Period (Weeks 21 to 48)

Changes in the Bacterial Diversity of Human Milk during Late Lactation Period (Weeks 21 to 48)

Probiotic characterization of lactic acid bacteria isolated from human milk

Human Milk Microbiota and Oligosaccharides: A Glimpse into Benefits, Diversity, and Correlations

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