Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

Abstract

Ulcerative colitis is an inflammatory disease of the intestine caused by many reasons, and it may even develop into colon cancer. Probiotics are normal bacteria that exist in the human body and have been proven to regulate the balance of intestinal flora and alleviate inflammation. The current study aimed to study the effect of Lactobacillus fermentum ZS40 (ZS40) on dextran sulfate sodium (DSS)-induced ulcerative colitis mice. The length and weight of the colon were measured, and the histopathological morphological changes of colon tissue were observed to evaluate the effects of ZS40 on colitis. Biochemical kits, ELISA kits, real-time quantitative PCR (RT-qPCR), and western blot were also used to detect the effects of ZS40 on serum and colon tissue related oxidative indicators and pro-inflammatory and anti-inflammatory cytokines. We found that ZS40 could reduce colonic inflammatory cell infiltration and goblet cell necrosis, increase total superoxide dismutase and catalase in mouse serum, and reduce myeloperoxidase and malondialdehyde levels. ZS40 could down-regulate the level of proinflammatory cytokines and up-regulate the level of anti-inflammatory cytokines. More importantly, ZS40 down-regulated the relative expression of nuclear factor-κB p65 (NF-κBp65), IL-6, and TNF-α mRNA and protein, up-regulated the relative expression of inhibitor kapa B alpha (IκB-α). By regulating the NF-κB and MAPK pathways to down-regulated the relative expression of p38 and JNK1/2 mRNA and p38, p-p38, JNK1/2, and p-JNK1/2 proteins. Our study suggested that ZS40 may serve as a potential therapeutical strategy for ulcerative colitis.

Prevention of DSS-induced colitis in mice with water kefir microbiotaviaanti-inflammatory and microbiota-balancing activity

“…57 The administration of water kefir microbiota effectively decreased the expression of TLR4, MyD88, and NF-κB, which was similar to previous studies which have shown that Lactobacillus, Saccharomyces, and Bifidobacterium can relieve colitis by decreasing the expression of TLR4, MyD88, and NF-κB. [58][59][60] These findings…”

Two novel lactic acid bacteria,Limosilactobacillus fermentumMN-LF23 andLactobacillus gasseriMN-LG80, inhibitedHelicobacter pyloriinfection in C57BL/6 mice

“…39 In addition, L. fermentum can down-regulate the level of inflammatory cytokines and up-regulate the level of anti-inflammatory cytokines in mice challenged with dextran sulfate sodium. 40 In this study, we found that both LF23 and LG80 significantly reduced the levels of inflammatory cytokine TNF-α, IL-1β and IL-6.…”

Probiotic-Based Intervention in the Treatment of Ulcerative Colitis: Conventional and New Approaches

“…Probiotics are able to attenuate inflammatory processes in UC by inhibiting the expression and production of pro-inflammatory transcription factors and/or molecules, and/or by inducing immunoregulatory mechanisms such as the differentiation of Th regulatory lymphocytes in the gut mucosa [ 69 ]. Several strains of Limosilactobacillus (formerly Lactobacillus ) fermentum have been shown to mitigate inflammatory processes by inhibiting the expression of pro-inflammatory transcription factors (nuclear factor-κB p65, mitogen-activated protein kinases p38 and JNK1/2) and downregulating the level of pro-inflammatory molecules (inducible nitric oxide synthase, cyclooxygenase 2) and cytokines (IL-6, TNF-α, IL-12) [ 69 , 70 , 71 ]. Other probiotic strains, such as Lactiplantibacillus (formerly Lactobacillus ) plantarum and Bifidobacterium longum , have been documented to alleviate chemically induced colitis by restoring the Th17/Treg balance in the lamina propria of the colon [ 72 ].…”