Lactobacillus from the Altered Schaedler Flora maintain IFNγ homeostasis to promote behavioral stress resilience

Merchak, Andrea R.; Wachamo, Samuel; Brown, Lucille C.; Thakur, Alisha; Moreau, Brett; Brown, Ryan M.; Rivet-Noor, Courtney R.; Raghavan, Tula; Gaultier, Alban

doi:10.1016/j.bbi.2023.11.001

Cited by 9 publications

(4 citation statements)

References 58 publications

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“…To complement this approach, we also examined the impact of stressed microbial signatures on mucin expression in germ-free mice. Our lab has successfully demonstrated that microbial signatures from stressed mice can induce despair and anxiety-like behaviors in germ-free mice ( Merchak et al, 2024 ). Therefore, using intestinal samples collected from germ-free mice which were given fecal pellets from naïve or stress-exposed animals, we again examined changes in mucin expression between groups by qPCR.…”

Section: Resultsmentioning

confidence: 99%

“…Animals were allowed to habituate to the introduced microbiome for 14 days from the first gavage. Germ-free experiments where animals received stress or naïve fecal pellets were described previously ( Merchak et al., 2024 ). Briefly, equal amounts of dirty bedding from naïve or stressed animals was transferred daily to germ-free animal cages for two weeks.…”

Section: Methodsmentioning

confidence: 99%

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Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors

Rivet-Noor,

Merchak,

Render

et al. 2024

Brain, Behavior, and Immunity

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors

Rivet-Noor,

Merchak,

Render

et al. 2024

Brain, Behavior, and Immunity

Self Cite

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“…This is supported by studies indicating that mice subjected to direct stress exhibit reduced levels of gut Lactobacillus. Among metabolites involved, it seems that the IFNγ, an inducer of tryptophan conversion to kynurenine produced in response to Lactobacillus, can be sufficient to provide resilience in response to environmental stressors [53,54]. Furthermore, the largest contributor among metabolites that the gut microbiota uses to signal the human brain is tryptophan and its precursors [55].…”

Section: Discussionmentioning

confidence: 99%

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Santonocito,

Paladino,

Vitale

et al. 2024

Biology

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Background: Dysbiosis, influenced by poor diet or stress, is associated with various systemic diseases. Probiotic supplements are recognized for stabilizing gut microbiota and alleviating gastrointestinal issues, like irritable bowel syndrome (IBS). This study focused on the tryptophan pathways, which are important for the regulation of serotonin levels, and on host physiology and behavior regulation. Methods: Nanovesicles were isolated from the plasma of subjects with chronic diarrhea, both before and after 60 days of consuming a probiotic mix (Acronelle®, Bromatech S.r.l., Milan, Italy). These nanovesicles were assessed for the presence of Tryptophan 2,3-dioxygenase 2 (TDO 2). Furthermore, the probiotics mix, in combination with H2O2, was used to treat HT29 cells to explore its cytoprotective and anti-stress effect. Results: In vivo, levels of TDO 2 in nanovesicles were enhanced in the blood after probiotic treatment, suggesting a role in the gut–brain axis. In the in vitro model, a typical H2O2-induced stress effect occurred, which the probiotics mix was able to recover, showing a cytoprotective effect. The probiotics mix treatment significantly reduced the heat shock protein 60 kDa levels and was able to preserve intestinal integrity and barrier function by restoring the expression and redistribution of tight junction proteins. Moreover, the probiotics mix increased the expression of TDO 2 and serotonin receptors. Conclusions: This study provides evidence for the gut–brain axis mediation by nanovesicles, influencing central nervous system function.

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“…In addition, oral administration of Bifidobacterium lactis ameliorates cognitive dysfunction in Aβ-injected mice through attenuation of ERK and JNK phosphorylation [ 55 ]. As well as it has been reported that Lactobacillus species act as protective from environmental stressors [ 56 ], and Bifidobacterium breve alleviated Aβ-induced cognitive impairment and enhanced synaptic function in AD mice [ 57 ]. We previously demonstrated that orally supplemented B. breve MCC1274 mitigated AD pathologies, as indicated by improved memory impairment, reduced amyloidosis and neuroinflammation, and improved synaptic plasticity, in both young AD model and WT mice [ 10 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Therapeutic Effects of Bifidobacterium breve MCC1274 on Alzheimer’s Disease Pathologies in AppNL-G-F Mice

Abdelhamid,

Jung,

Zhou

et al. 2024

Nutrients

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We previously demonstrated that orally supplemented Bifidobacterium breve MCC1274 (B. breve MCC1274) mitigated Alzheimer’s disease (AD) pathologies in both 7-month-old AppNL-G-F mice and wild-type mice; thus, B. breve MCC1274 supplementation might potentially prevent the progression of AD. However, the possibility of using this probiotic as a treatment for AD remains unclear. Thus, we investigated the potential therapeutic effects of this probiotic on AD using 17-month-old AppNL-G-F mice with memory deficits and amyloid beta saturation in the brain. B. breve MCC1274 supplementation ameliorated memory impairment via an amyloid-cascade-independent pathway. It reduced hippocampal and cortical levels of phosphorylated extracellular signal-regulated kinase and c-Jun N-terminal kinase as well as heat shock protein 90, which might have suppressed tau hyperphosphorylation and chronic stress. Moreover, B. breve MCC1274 supplementation increased hippocampal synaptic protein levels and upregulated neuronal activity. Thus, B. breve MCC1274 supplementation may alleviate cognitive dysfunction by reducing chronic stress and tau hyperphosphorylation, thereby enhancing both synaptic density and neuronal activity in 17-month-old AppNL-G-F mice. Overall, this study suggests that B. breve MCC1274 has anti-AD effects and can be used as a potential treatment for AD.

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Lactobacillus from the Altered Schaedler Flora maintain IFNγ homeostasis to promote behavioral stress resilience

Cited by 9 publications

References 58 publications

Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors

Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Potential Therapeutic Effects of Bifidobacterium breve MCC1274 on Alzheimer’s Disease Pathologies in AppNL-G-F Mice

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