Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Abstract

Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gasseri LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.

Lactobacillus gasseri JM1 with potential probiotic characteristics alleviates inflammatory response by activating the PI3K/Akt signaling pathway in vitro

“…Importantly, the genome encoded 3 gene clusters (JM1_GM000623-JM1_GM000640, JM1_GM001955-JM1_GM001967, and JM1_GM002030-JM1_GM002041) for bacteriocin biosynthesis. The results indicated that L. gasseri JM1 possessed the capacity to resist oxidative stress and synthesize bacteriocin, which was in accordance with previous reports (Zorič Peternel et al, 2010;Maldonado-Barragán et al, 2016;Hu et al, 2018). Compared with other L. gasseri strains, the genetic relationship of L. gasseri JM1 with 4M13 and K7 is closer, which may associate to the origin.…”

Lactobacillus frumenti mediates energy production via fatty acid β-oxidation in the liver of early-weaned piglets

“…Growing evidence suggests that the gut microbiota regulate host energy metabolism. Our previous study demonstrated that Lactobacillus gasseri LA39 may act as a potential probiotic and stimulates energy production in early-weaned piglets [21]. The present study provides new insights into the effects of L. frumenti to increase the β-oxidation of fatty acid in the liver of early-weaned piglets.…”

Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde

“…V-type proton ATPase subunit a (protein accession number H0YEL3, FC = 0.00) was markedly damaged by CRA, although it is closely related to energy production promotion and confers health-promoting functions in porcine intestinal epithelial cells [ 28 ]. Upregulation (FC = 1.82) of the mitochondrial inner membrane protein OXA1L (protein accession number E7EVY0) effectively promotes the assembly of multiple respiratory chain complexes [ 29 ], while increasing the expression (FC = 1.88) of glutamate-cysteine ligase modifier (GCLM) (protein accession number P48507), which promotes the expression of GSH.…”