Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds

Abstract

Two strains, KBL13T and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13T and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA–DNA hybridization, DNA G+C content and biochemical characterization indicated that these two strains, KBL13T and GBL13, belong to the same species. In the representative strain, KBL13T, the DNA G+C content was 34.3 mol%. Lactobacillus salivarius JCM 1231T (=ATCC 11741T; AF089108) is the type strain most closely related to the strain KBL13T as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0 % (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA–DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13T represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13T (=JCM 14209T=DSM 18933T).

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

“…Of these, only two bands were found to be common in all five horses. According to BLAST of near full-length 16S rRNA gene sequences (41455 nt) the clones that formed these bands had the highest identity with Lactobacillus equi (AB048833: 99.4%) and Lactobacillus hayakitensis (AB267406: 99.7%), which are considered to be commensals of the equine gastrointestinal tract (Morotomi et al, 2002;Morita et al, 2007). Intensification of the lactobacilli bands was not as marked as for other species, such as the EHSS and E. coli 3, and appeared to be secondary to EHSS proliferation (Figure 1).…”

Equine Stomachs Harbor an Abundant and Diverse Mucosal Microbiota

“…The most abundant read type in the squamous region of horse H3 was a Streptococcus species; however, these reads did not cluster with a specific type strain. The most dominant read type in the ulcerated region of horse H3 was found in a well-supported (BS of 87) cluster with another horse-associated species, Lactobacillus equigenerosi (22,42). Although Helicobacter spp.…”

Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse

“…The G+C content of strain DI70 T was determined in three separate trials by using previously described methods (Morita et al, 2007b (Table 1). However, the DNA G+C content of DI70…”

“…The diversity of lactobacilli in the contents of the equine gastrointestinal tract and faeces has been investigated using culturing and molecular profiling methods, respectively (Al Jassim et al, 2005;Endo et al, 2007). The species Lactobacillus equi, L. hayakitensis and L. equigenerosi were first isolated from the equine gastrointestinal tract/faeces (Morotomi et al, 2002;Morita et al, 2007b;Endo et al, 2008). L. equi and L. hayakitensis are placed within the Lactobacillus salivarius phylogenetic group and L. equigenerosi within the Lactobacillus reuteri phylogenetic group.…”

“…The 16S rRNA gene sequence (1486 bp) of strain DI70 T was determined by using previously described methods (Morita et al, 2007b). The closest known relatives of the isolate were determined by performing database searches, and the sequences of closely related species were retrieved from public databases.…”