Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis

Yuan, Long; Zhu, Chuyang; Gu, Fang; Zhu, Miaonan; Yao, Jiacheng; Zhu, Cuipeng; Li, Shicheng; Wang, Kun; Hu, Ping; Zhang, Yunzeng; Cai, Demin; Liu, Haoyu

doi:10.1016/j.aninu.2023.04.012

Cited by 3 publications

(6 citation statements)

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“…This capability enhances the precise and confident identification of genes associated with probiotic properties and other traits through genome mining, as well as genes linked to evolution, including those related to host adaptation [17,18]. L. johnsonii was suggested to be positively associated with the ability to resist chronic heat stress-induced diarrhea in weaned piglets, as revealed by comparative microbiome profiling analysis in our previous study [19]. Then, a total of 49 L. johnsonii strains were isolated from the feces of these heat-stress-tolerant piglets.…”

Section: Introductionmentioning

confidence: 71%

“…In our previous study, we found that L. johnsonii exhibited a relatively high abundance in the microbiota (>15%) of weaned piglets that were resistant to heat stress-induced diarrhea, while it was almost undetectable in the microbiota of those who had diarrhea [19]. The result suggested L. johnsonii probably exhibited probiotic effects to maintain the health of the host.…”

Section: Probiotic Properties Of L Johnsonii Strains Isolated From He...mentioning

confidence: 90%

“…The LAB were isolated from the feces of weaned piglets that exhibited resistance to chronic heat stress (i.e., no diarrhea symptom) [19] through a dilution and plate coating method. Specifically, 2 g of each swine fecal sample was weighed and then added to a sterilized tube that contained 5 mL of sterilized PBS.…”

Section: Isolation Of Lactic Acid Bacteria From Swine Fecesmentioning

confidence: 99%

“…The results demonstrated that the growth activity of N5 and N7 in the acidic and bile environments gradually declined over time, while still maintaining a relatively high survival rate. Then, strain N5 was selected for in vivo probiotic capacity assessment using a mouse model, and the results demonstrated that oral administration of strain N5 alleviated the clinical and histological signs of colitis of the host through several means, including suppressing the expression of pro-inflammatory cytokines TNF-α and IL-6 within the intestinal tract [19].…”

Section: Probiotic Properties Of L Johnsonii Strains Isolated From He...mentioning

confidence: 99%

“…Then, a total of 49 L. johnsonii strains were isolated from the feces of these heat-stress-tolerant piglets. A representative L. johnsonii strain, N5, was selected for in vivo experiments, and this strain was demonstrated to exhibit protective effects against dextran sulfate sodium (DSS)-induced colitis of the host by strengthening intestinal barrier function and balancing the response of intestinal innate and adaptive immunity [19]. In the current study, we conducted in vitro assessments of the antagonistic activity against pathogenic bacteria and the tolerance to gastric acid and bile of the L. johnsonii strains.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Probiotic Properties and Whole-Genome Analysis of Lactobacillus johnsonii N5 and N7 Isolated from Swine

Wang,

et al. 2024

Microorganisms

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In our previous microbiome profiling analysis, Lactobacillus (L.) johnsonii was suggested to contribute to resistance against chronic heat stress-induced diarrhea in weaned piglets. Forty-nine L. johnsonii strains were isolated from these heat stress-resistant piglets, and their probiotic properties were assessed. Strains N5 and N7 exhibited a high survival rate in acidic and bile environments, along with an antagonistic effect against Salmonella. To identify genes potentially involved in these observed probiotic properties, the complete genome sequences of N5 and N7 were determined using a combination of Illumina and nanopore sequencing. The genomes of strains N5 and N7 were found to be highly conserved, with two N5-specific and four N7-specific genes identified. Multiple genes involved in gastrointestinal environment adaptation and probiotic properties, including acidic and bile stress tolerance, anti-inflammation, CAZymes, and utilization and biosynthesis of carbohydrate compounds, were identified in both genomes. Comparative genome analysis of the two genomes and 17 available complete L. johnsonii genomes revealed 101 genes specifically harbored by strains N5 and N7, several of which were implicated in potential probiotic properties. Overall, this study provides novel insights into the genetic basis of niche adaptation and probiotic properties, as well as the genome diversity of L. johnsonii.

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Section: Introductionmentioning

confidence: 71%

Section: Probiotic Properties Of L Johnsonii Strains Isolated From He...mentioning

confidence: 90%

Section: Isolation Of Lactic Acid Bacteria From Swine Fecesmentioning

confidence: 99%

Section: Probiotic Properties Of L Johnsonii Strains Isolated From He...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Probiotic Properties and Whole-Genome Analysis of Lactobacillus johnsonii N5 and N7 Isolated from Swine

Wang,

et al. 2024

Microorganisms

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The influence of Lactobacillus johnsonii on tumor growth and lymph node metastasis in papillary thyroid carcinoma

Min,

Xie,

Yang

et al. 2024

Preprint

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The presence of lymph node metastasis (LNM) is one of the most crucial factors in the prognosis of papillary thyroid carcinoma (PTC). The aim of this study was to investigate the influence of intratumoral bacteria on the LNM in PTC. The intrathyroidal microbiome was analyzed in 55 PTC patients by 16S rRNA gene sequencing. The CCK8 and Transwell assays were conducted to determine the impact of bacteria on the proliferation and migration abilities of PTC cells. Xenograft tumor and bacterial colonization experiments were carried out using nude mice. We showed that Lactobacillus was significantly decreased in PTC lesions from patients with LNM. Lactobacillus johnsonii (L. johnsonii) could suppress the proliferation and migration capability of PTC cells in vitro and in vivo. Bacterial gut colonization of L. johnsonii increased its abundance in tumors and inhibited PTC growth and LNM. These findings suggested that L. johnsonii could be harnessed for the development of innovative therapeutic strategies for PTC.

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Proton pump inhibitor alters Th17/Treg balance and induces gut dysbiosis suppressing contact hypersensitivity reaction in mice

Strzępa,

Marcińska,

Kiecka

et al. 2024

Front. Immunol.

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Proton pump inhibitors (PPIs), such as omeprazole, are the most commonly prescribed drugs. Treatment with PPIs alters gut microbiota composition and reduces the production of reactive oxygen (ROS) and proinflammatory IL-1β, IL-6, and TNF-α cytokines. Here, using the T cell-dependent contact hypersensitivity (CHS) response, an animal model of allergic contact dermatitis (ACD) that affects up to 30% of the population, we demonstrated that a two-week omeprazole treatment suppresses the development of CHS. Omeprazole treatment before CHS induction, reduced inflammatory response in ears measured by ear swelling, ear biopsy weight, MPO activity, and proinflammatory cytokine production. These changes were associated with reduced frequency of TCRαβ+ CD4+ IL-17A+ and TCRαβ+ CD8+ IL-17A+ T cells and increased frequency of TCRαβ+ CD4+ CD25+ FoxP3+ Treg, and TCRαβ+ CD4+ IL-10+ Tr1 cells in peripheral lymphoid organs. Omeprazole treatment decreased the production of ROS, TNF-α, and IL-6, which supported Th17 cell induction, and increased the frequency of Clostridium cluster XIVab and Lactobacillus, implicated in Treg cell induction. The fecal microbiota transplantation (FMT) experiment confirmed the role of omeprazole-induced changes in gut microbiota profile in CHS suppression. Our data suggests that omeprazole ameliorates inflammatory response mediated by T-cells.

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Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis

Cited by 3 publications

References 59 publications

Characterization of Probiotic Properties and Whole-Genome Analysis of Lactobacillus johnsonii N5 and N7 Isolated from Swine

Characterization of Probiotic Properties and Whole-Genome Analysis of Lactobacillus johnsonii N5 and N7 Isolated from Swine

The influence of Lactobacillus johnsonii on tumor growth and lymph node metastasis in papillary thyroid carcinoma

Proton pump inhibitor alters Th17/Treg balance and induces gut dysbiosis suppressing contact hypersensitivity reaction in mice

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