Lactobacillus paragasseri as a novel causative pathogen of cavernosal abscess

Toyoshima, Hirokazu; Shibahara, Takuji; Tanigawa, Motoaki; Masuda, Naoto; Ishiguro, Chiaki; Tanaka, Hiroyuki; Nakanishi, Yuki; Sakabe, Shigetoshi

doi:10.1016/j.idcr.2021.e01320

Cited by 3 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lactobacilli were isolated from blood [ 15 , 16 , 17 , 18 , 20 , 21 , 22 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 49 , 52 , 55 , 56 , 57 ], diseased valve tissue [ 23 ], abscess fluids [ 48 , 51 , 52 , 54 , 57 , 58 ], cerebrospinal fluid (CSF) [ 46 ], marrow [ 30 ], arthrocentesis aspiration fluid [ 53 ] in anaerobiosis and, in some cases, also in aerobiosis [ 15 , 34 ]. Use of automated microbial detection system, i.e., BACT/ALERT 3D or the BACTEC systems (BioMérieux) with incubation at 35 °C were mentioned in some reports [ 23 , 31 , 34 ].…”

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

“…Sequencing of the 16S rRNA gene was carried out in six instances and whole genome sequencing in two ( Table S1 ). In one case there was discordance in the identification with MALDI TOF mass spectrometry and 16S rRNA gene sequencing between the strictly related species L. gasseri and L. paragasseri [ 58 ].…”

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

“…Susceptibility to β-lactams, including amoxicillin, amoxicillin/clavulanic acid, ampicillin, ampicillin-sulbactam, benzylpenicillin, penicillin G, piperacillin/tazobactam, and meropenem IV was reported in most cases. These antibiotics were used singly or in association with gentamicin, clindamycin, and clarithromycin [ 16 , 17 , 20 , 21 , 22 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 35 , 36 , 37 , 38 , 39 , 40 , 43 , 44 , 45 , 46 , 48 , 49 , 51 , 53 , 54 , 55 , 56 , 57 , 58 ].…”

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

See 2 more Smart Citations

Lactobacilli Infection Case Reports in the Last Three Years and Safety Implications

et al. 2022

View full text Add to dashboard Cite

Lactobacilli constitute the dominant microbiota in many fermented foods and comprise widely used probiotics. However, these bacteria cause rare infections mostly in diabetic and immunocompromised subjects in presence of risk factors such as prosthetic hearth valves and dental procedures or caries. The scope of this survey was re-assessing the pathogenic potential of lactobacilli based on the infection case reports published in the last three years. In 2019, 2020, and 2021, total of 17, 15, and 16 cases, respectively, including endocarditis, bacteremia, and other infections, were reported. These annual numbers are higher than those observed previously. Lacticaseibacillus rhamnosus (13 cases), comprising strain GG (ATCC 53103) with established applications in healthcare, L. paracasei (7 cases), Lactobacillus acidophilus (5 cases), L. jensenii (5 cases), Lactiplantibacillus plantarum (3 cases), L. paraplantarum, L. delbrueckii subsp. delbrueckii, L. gasseri, L. paragasseri, Limosilactobacillus fermentum, and L. reuteri (1 case each) were involved. Virulence characterization of two strains that caused infections, a derivative of L. rhamnosus GG and L. paracasei LP10266, indicated that increased biofilm-forming capacity favors pathogenicity and it is determined by variable genetic traits. This survey highlights that the strains of lactobacilli that cause infections are little characterized genetically. Instead, to avoid that these bacteria become a hazard, genetic stability should be periodically re-evaluated by whole genome sequencing (WGS) to ensure that only non-pathogenic variants are administered to vulnerable individuals.

show abstract

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

Section: Cases Of Lactobacilli Infections Reported In Years 2019 2020...mentioning

confidence: 99%

See 1 more Smart Citation

Lactobacilli Infection Case Reports in the Last Three Years and Safety Implications

et al. 2022

View full text Add to dashboard Cite

show abstract

Ultrasound-guided puncture and drainage for penile abscess: Case report and review of the literature

Yuan

Wang

et al. 2023

Radiology Case Reports

View full text Add to dashboard Cite

Genomic insights into Lactobacillus gasseri and Lactobacillus paragasseri

Ene

Stegman

Wolfe

et al. 2022

PeerJ

View full text Add to dashboard Cite

Background Antimicrobial and antifungal species are essential members of the healthy human microbiota. Several different species of lactobacilli that naturally inhabit the human body have been explored for their probiotic capabilities including strains of the species Lactobacillus gasseri. However, L. gasseri (identified by 16S rRNA gene sequencing) has been associated with urogenital symptoms. Recently a new sister taxon of L. gasseri was described: L. paragasseri. L. paragasseri is also posited to have probiotic qualities. Methods Here, we present a genomic investigation of all (n = 79) publicly available genome assemblies for both species. These strains include isolates from the vaginal tract, gastrointestinal tract, urinary tract, oral cavity, wounds, and lungs. Results The two species cannot be distinguished from short-read sequencing of the 16S rRNA as the full-length gene sequences differ only by two nucleotides. Based upon average nucleotide identity (ANI), we identified 20 strains deposited as L. gasseri that are in fact representatives of L. paragasseri. Investigation of the genic content of the strains of these two species suggests recent divergence and/or frequent gene exchange between the two species. The genomes frequently harbored intact prophage sequences, including prophages identified in strains of both species. To further explore the antimicrobial potential associated with both species, genome assemblies were examined for biosynthetic gene clusters. Gassericin T and S were identified in 46 of the genome assemblies, with all L. paragasseri strains including one or both bacteriocins. This suggests that the properties once ascribed to L. gasseri may better represent the L. paragasseri species.

show abstract

Lactobacillus paragasseri as a novel causative pathogen of cavernosal abscess

Cited by 3 publications

References 7 publications

Lactobacilli Infection Case Reports in the Last Three Years and Safety Implications

Lactobacilli Infection Case Reports in the Last Three Years and Safety Implications

Ultrasound-guided puncture and drainage for penile abscess: Case report and review of the literature

Genomic insights into Lactobacillus gasseri and Lactobacillus paragasseri

Contact Info

Product

Resources

About