Lactobacillus plantarum CQPC11 Isolated from Sichuan Pickled Cabbages Antagonizes d-galactose-Induced Oxidation and Aging in Mice

Abstract

Chinese pickled cabbage is a traditional fermented food that contains abundant microbes produced during the process of fermentation. In this work, an in vivo animal study was conducted to investigate the effects of a newly isolated lactic acid bacterium (Lactobacillus plantarum CQPC11, LP-CQPC11) on d-galactose-induced oxidation and aging in mice. Analysis of the serum and tissue samples of these mice using molecular biology approaches showed that LP-CQPC11 suppressed the decrease in thymus, brain, heart, liver, spleen, and kidney indices caused by oxidation and aging. Furthermore, LP-CQPC11 increased the levels of SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), and GSH (glutathione), whereas it reduced the levels of NO (nitric oxide) and MDA (malondialdehyde) in the serum, liver, and spleen of oxidation and aging mouse models. Pathological observation indicated that LP-CQPC11 alleviated the damage caused by oxidation and aging on the liver and spleen of mice. qPCR analysis indicated that LP-CQPC11 effectively upregulated the expression of nNOS (neuronal nitric oxide synthase), eNOS (endothelial nitric oxide synthase), Cu/Zn-SOD (cuprozinc-superoxide dismutase), Mn-SOD (manganese superoxide dismutase), CAT (catalase), HO-1 (heme oxygenase-1), Nrf2 (nuclear factor-erythroid 2 related factor 2), γ-GCS (γ-glutamylcysteine synthetase), and NQO1 (NAD(P)H dehydrogenase [quinone] 1), but downregulated the expression of iNOS (inducible nitric oxide synthase) in the mouse liver and spleen. Western blot analysis showed that LP-CQPC11 effectively upregulated SOD1 (Cu/Zn-SOD), SOD2 (Mn-SOD), CAT, GSH1 (c-glutamylcysteine synthetase), and GSH2 (glutathione synthetase) protein expression in mouse liver and spleen tissues. These findings suggest that LP-CQPC11 can effectively prevent d-galactose-induced oxidation and aging in mice, and the effect is even better than that of the commonly used Lactobacillus delbruechii subsp. bulgaricus (LDSB) and vitamin C in the industry. Thus, LP-CQPC11 may be potentially employed as a probiotic strain.

Lactobacillus plantarum KSFY06 on d‐galactose‐induced oxidation and aging in Kunming mice

“…From the mouse liver tissue measurement indicators GSH-Px and CAT (Table 5), vitamin C was as good as KSFY06-H in the antioxidant effect. Qian et al (2018) found that MDA activity in the serum and liver of antioxidized mice was decreased though L. plantarum CQPC-11 was administered. Wa et al (2019) found that probiotics significantly improved the antioxidant capacity of hyperlipidemic mice and increased the activity of antioxidant enzymes including SOD, GSH-PX, GPT, and GOT.…”

“…Studies have shown that the liver index of aging mice is significantly lower than that of normal nonaging mice (Sang et al, 2017). Therefore, changes in the structure and function of mouse organs can be ob- Skin, spleen, and liver can reflect some of the characteristic changes that occur in the body due to aging (Qian et al, 2018). The spleen is closely related to the cellular immune response of animals, but the immune ability of aging mice is relatively weak.…”

Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice

“…Gastrointestinal hormones, such as MTL and gas, exert direct influences on constipation, and the measurement of these indices can evaluate the degree of constipation [25,26]. MTL can improve stomach peristalsis, leading to the secretion of hydrochloric acid, pancreatic juice, and bile, which reflects gastrointestinal motility to a certain degree.…”

Preventive effect of insect tea primary leaf (Malus sieboldii (Regal) Rehd.) extract on D‐galactose‐induced oxidative damage in mice

“…An additional 5 L of distilled water was added to the final product residue, and it was re‐extracted at 80°C for 2 hr. The two filtrates were combined, the methanol was removed with a rotary evaporator, and the extracts were collected (Qian et al., 2018).…”

“…The mice were divided into five groups. The experiment was conducted for 10 weeks (Figure 1), after which all of the mice were killed following a 24‐hr fast (Qian et al., 2018). Serum samples were obtained from the heart, and samples of the internal organs were excised for the experiment.…”